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  1. Summer Fun at Addgene!

    Type
    Blog Post
    ...Series. We’ll be covering the basics of science communication, tips and tricks for sharing your science with...Addgene TikTok at the end of this month! We’ll be sharing our educational resources and tips and tricks for... now! (Bonus: Tim kindly offered to finishing answering all submitted questions via blog post, which we...

  2. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...CRISPR during his postdoc 12:01 - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why...the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had on the podcast... of medical school. 7:05 - 10:55 : Alex's focus during graduate school - DNA damage and longevity. 10:...

  3. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...I can bring to Addgene -- that comfort with saying, “if we do this, then what?” I hope to bring that business...my personality. Another big part of my role is bringing experience and judgement to a wide variety of ...business world from the mindset of a lawyer can bring a new perspective to an organization. I'm used to...

  4. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - Developing Addgene’s ...Future, the research team, and the importance of sharing information/knowledge Outro Many thanks to our...received his Bachelor of Science degree in Computer Engineering from Tufts University and his Master of Arts ...Bioworks. He also holds a SB and a Masters in Engineering from MIT, both in Computer Science.   Special...

  5. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ... grown steadily during 2014. We were excited to have more and more scientists offering to share their ...Will You Be My Mentor? Finding and Asking for Mentoring Support By Joanne Kamens Plasmids 101: Antibiotic...Margo R. Monroe Best wishes for your research during this holiday season and we look forward to seeing...

  6. Antibodies 101: Validation

    Type
    Blog Post
    ...protein can also influence antibody binding by altering the relative concentration of potential antibody...to validate that your antibody is specifically capturing your RNA-binding protein of interest. So you ...antibody’s trustworthiness. You may also consider sharing your validation attempts by other means, such as.... These outlets can be particularly useful for sharing negative data. Negative data often goes overlooked...antibody (perhaps one from Addgene!), consider sharing your results so that others in the community can...

  7. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...are thought to interrogate dsDNA for proper base pairing through minor grove contacts, and thus do not read... do with the slight melting of the 5 ́-terminus during the reaction. This “peeling back” of the 5 ́-phosphorylated...upstream probes and four down-stream probes, each differing only in the base at the ligation junction. Thus...prepared, each with a different complement strand covering all 16 possible NN pairs in the template. The ...The probes were designed such that each possible pairing resulted in a product of a different length, with...

  8. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...on how they've managed to keep themselves happy during their time in grad school. As you'll learn, grad... hard about picking a good mentor, and begin considering career options early. Tune in for great advice... in grad school? As part of this discussion, we bring up a performance Ben and Niroshi organized for Tyler's...Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and Ben for taking...

  9. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...can help with plasmid sharing! Judging by how many prizes were given out during the lunchtime pizza and...see a real working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite... that are offered with a biology major.” After hearing about many Addgenies’ career paths, Michelle Healy...

  10. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...articles appearing in lower-prestige journals has risen steadily from 1995 to 2013. Considering citations...publications alone aren’t enough. Scientists are sharing what they call “raw science” – data sets, code,...researchers to track and share their own libraries and offering another way to consider and quantify the impact...

  11. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that...haven’t already used it yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR...work probing mTOR signaling in cancer cells, deciphering the mechanism of matrix metalloproteinase-9 secretion...Addgene and look forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale...

  12. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...focused on our core mission to promote scientific sharing. Listen to learn more about Darrell, his research...repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary nature of the Center...practices and promotes open science in the lab - sharing reagents, cell lines, data, and knowledge without...Addgene board of directors - increasing open source sharing and helping us stick to our core mission. Chapter...

  13. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...cells using flow cytometry, you are typically measuring the presence or absence of certain markers on ...photon is emitted. This step is called emission. During excitation, some of the photon's energy is lost... red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation spectra around...you can see in Figure 1, emission spectra of neighboring colors can and do overlap. For example, APC overlaps...hypothetical experiment in the lab. Let's say I am culturing HEK 293T cells in vitro. I transduced the cultured...

  14. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...in order to directly show that, for example, the firing of a specific type of neuron in a specific brain...whether you wish to stimulate or inhibit neuronal firing, the cell-type specificity, the kinetics of the... which results in depolarization and subsequent firing of an action potential. Halorhodopsins (HR) are...may more effectively silence neurons than HR. Delivering your opsin  The standard opsin delivery method...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

  15. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an... experiments. Addgene’s dedication to resource sharing has led us to develop a searchable and sortable...can search by any part of the data. For example, entering ‘C. elegans’, or ‘elegans’ will filter the table...CRISPR Technologies Available Through Resource Sharing. 2016. In press. Additional Resources on the Addgene...

  16. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...unmatched customer support, as this is vital to ensuring that our services help accelerate your research...support team that can help you through any search, ordering, technical, or quality control issues. Improving...within the widget, you’ll navigate to articles answering your questions making it easier than ever to get...describe your issue and press send. Doing so will bring your concern directly to our support team’s attention...

  17. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ..., Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating...normally and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing...and relevance for the efficiency of BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva... Mary Gearing...

  18. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...success.” 2. “Enjoy Teamwork.” Be friendly, open, sharing, rather than intensely competitive. Be curious ...society. We need much, much more science and engineering (STEM) expertise in law, business, journalism...connected to scientists and use the skills they learned during their studies. They suggest that you make time ...Connie Cepko, Tom Ellis, and George Church for answering our burning questions about how they manage and...

  19. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...can remain present until cleared by the body. Administering the drug once can result in several hours of... Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to...injection. The main challenge in using ligands is ensuring the agonist is able to cross the blood brain barrier...Bryan Roth lab for feedback on this article and sharing their expertise with us. References Urban DJ, Roth...

  20. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...hydrogen bonds, destabilizing the molecule and favoring the separation of the two helixes. Low pH instead...chromosome. One of the main causes of degradation during plasmid DNA extraction and purification from E....though in their capacity to maintain DNA stability during storage over prolonged periods of time, especially...especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should consider a couple...

  21. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...grow. Auxotrophy and prototrophy come into play during cloning if you’ve designed your experiment using...sucrose. Plasmid cloning: Creativity with naturally occurring genes  At this point in the blog post, you may...negative selection strategies adopt naturally occurring genes for cloning purposes (antibiotic resistance... 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection Resources...

  22. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...CASTs quickly became popular bacterial genome engineering tools, with early reports of 40%–60% efficient... & Sternberg, S. H. (2024). Bacterial genome engineering using CRISPR-associated transposases. Nature ... Berríos, K. N., McElroy, A. N., King, R. T., Stringham, O. G., Gelsinger, D. R., Vo, P. L. H., Chen, ...the Addgene blog INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Degrading DNA with ...

  23. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to stabilize...stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps capture...reducing the read depth necessary when sequencing and lowering the amount of tissue needed per experiment. In...transcriptional regulatory elements is controlled during development using the Drosophila wing as a model...

  24. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...stage you could infer that the gene which GFP was monitoring was being expressed in the same pattern. These...result but will still allow for expression pattern monitoring. Enhancer traps Like promoter traps, enhancer...unambiguously identify the gene that they are monitoring as they are directly integrated into it. Enhancer...e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007...

  25. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ... category already has five blue flame plasmids covering three different epigenetic effectors. The diversity...Bacteria: CRISPR Methods for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing...CRISPR History and Development for Genome Engineering  ...  Mary Gearing...

  26. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...speaker biographies.” During the week of our interview, Hans was also busy preparing materials for an upcoming...took that gamble, and it’s paid off. Work is never boring. “I just keep learning on the job. It keeps it ...mechanism that also looks nice is a great way to bring people in.” Are you interested in careers outside...

  27. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Vandenberghe. We’re excited to grow the team and bring on scientists with a diverse set of experiences....scientist that helps with different aspects of manufacturing and quality, especially with regard to compliance...journey in biochemistry made the foray into genetics during graduate school at MIT. “I read some of the seminal...He’s also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...

  28. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...researcher and ones that happen naturally.  Sample Denaturing  First, let’s think about induced conformation...s configuration in your assay. Did you have a denaturing step? Do you want to capture the protein bound...conditions? It can help to visualize an abstract protein during your sample prep process, imagining how it starts...antibodies that have been validated solely for denaturing assays. Many antibodies will note if they’re ...

  29. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...molecular definition, and potential for further engineering as compared to polyclonal or monoclonal antibodies... in this blog post. Hello, NABOR! Addgene will bring the same open-access benefits to antibodies that...collection. I’m thrilled to be working with Addgene to bring this resource to life,” says Dr. Trimmer. But in...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim Executive...

  30. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... each day brings in new and surprisingly diverse manuscripts. I work with people, nurturing networks, ...regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests, though,...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...

  31. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...academic positions. More recently, I have been hearing exit survey data from postdoctoral programs in ... (every 4-6 years is common), but this can also bring new opportunities—projects end and layoffs occur...change entire culture) Specific requirements for hiring more common Likelier to hire on general talent... paths is an important first step. Developing mentoring relationships, especially with peer mentors, to...

  32. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by ...ordered biochemical events” such as those that occur during development. Read this post to learn more about...research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica Polka,...consider if you’re getting started with them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening...

  33. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C. elegans were released, signaling...revolution!   References Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette....strategies for CRISPR/Cas9 editing in C. elegans: Engineering the Caenorhabditis elegans genome using Cas9-... Mary Gearing...

  34. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Griesbeck is motivated by a particular interest in monitoring the activity and biochemistry of living neurons...allowed the researchers to see tonic action potential firing in neurons and high resolution functional tracking...while in principle adapting a FRET sensor for monitoring calcium versus zinc should require the simple...cells at much lower concentrations than calcium, requiring greater sensitivity. He and his colleagues came...

  35. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...innovations have improved the odds of mismatch repair favoring the edited strand. The first iteration of prime...introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch repair Even in PE3 and PE3b, prime editing...of the unedited strand Favor the edited strand during mismatch repair   ✓   ✓ ~ ~ ~ ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,...recombinases and prime editing. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-024-01227-1 Yan...

  36. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Old Dominion University, who is majoring in marine biology and minoring in studio arts photography.  References...specific procedural instructions, including timing, preparing the smear, and mixing the stains and solutions.... & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland...

  37. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...limitations: they were not optimal for experiments requiring high signal to noise ratios, imaging of high frequency...Create yellow (v82) and lime green (v857) variants differing in kinetics, affinity, and ∆F/F0. Introduce the...and localization through different C-terminal anchoring domains The original iGluSnFR in the pMinDisplay...researchers introduce iGluSnFR3 in two additional anchoring domains – GPI and SGZ. Fig: 4 Sequence...

  38. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ... review, and more lab research could be used to bring scientists and the public together to build consensus... it is followed. He’s PCSK9 paper, though not featuring a clinical pregnancy, was rejected by at least...Download the CRISPR 101 eBook Read our genome engineering blog posts Resources on Addgene.org Read our...CRISPR plasmids by function Check out our genome engineering guide ...

  39. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary feature... traits. The “biotech yeast” You’re probably wondering if the “biotech yeast” is S. pombe or S. cerevisiae...small, membrane-bound organelles essential for sequestering certain enzymes and performing specific oxidative...

  40. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution stock...sequencing. Maintain representation of the library during transformation First, use enough DNA and competent... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...making sure few/no plasmids have dropped out, and comparing pre- and post-amplification sequencing results...
Showing: 441 - 480 of 1006 results