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  3. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...efficiency and any unpaired fluorescent proteins can contribute additional noise to the measurement. If the distance... FRET experiments are expected to continue to contribute to our understanding of diseases and even assist...

  4. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...plays an important role in RNA processing and contributes to variability in RNA half-life, and ultimately... Rho-independent termination is also known as intrinsic termination, and it relies on the formation of...

  5. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...Cardiovascular and diabetes mortality determined by nutrition during parents' and grandparents' slow growth ...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...genes for drug discovery or to understand the intricacies of molecular pathways. These screens have gotten...solutions have been found to get around this restriction. The most obvious workaround is to use a multi-plasmid...

  7. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps... next poster session! There many other tips and tricks for presenting at scientific conferences, so let...

  9. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...Updated Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca... academic lab is closely involved with clinical trials or has ties to industry, the drug discovery process...

  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...standard curves, and normal controls must be run in triplicate.  The nice thing about qPCR is that since it ... of reactions, it does not need to be run in triplicates. ddPCR is an extremely accurate application, ...

  12. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...This post was contributed by guest blogger Iris Lindberg, Professor at the University of Maryland School...Cloning cells using an agarose overlay does have one tricky step. It is necessary to pour the agarose onto ...

  13. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...relative nuclease efficiencies as well as to more stringent targeting rules that result in a decrease in available...

  15. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...are treated with a drug of interest and gRNA distribution is analyzed in the drug-resistant population...6 gRNAs per target gene, and maintaining the distribution of each gRNA within the population is key. Loss...

  16. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to get around...

  17. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...multicistronic vectors work? Scientists have "borrowed" some tricks discovered in positive single-stranded RNA viruses...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

  18. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... short as 100ms (EBFP) or as long as 1 hour (mAmetrine1.2). However, for most FPs it is a few seconds ...

  19. An Introduction to Adenovirus

    Type
    Blog Post
    ...noticing unusual cases of acute hepatitis in the pediatric population. Once ~400 cases had amassed in places...removal of certain adenoviral genes that apparently contribute to virulence (Tollefson et al., 1996) – cell ...

  20. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...’ve got you covered in this blog, with tips and tricks for harnessing the homology-directed repair pathway...are overly confluent or don’t have sufficient nutrients in their media, they will not undergo DNA replication...

  21. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...conditions commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76. https...Qu, G. (2005). Identification of factors that contribute to recombinant AAV2 particle aggregation and ...

  22. Tips for arabidopsis transformation

    Type
    Blog Post
    ...This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic... it pretty much is, but there are some tips and tricks you can use to obtain optimum transformation efficiency...

  24. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...functions differently. Since Cpf1 loci are widely distributed across bacterial species, Zetsche et al. hypothesized...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

  25. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national...professionals is learning to succeed in grants through trial and error, through winning and losing funding, and...

  27. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...an elderly family member, want to train for a triathlon, finally buy that charming fixer-upper house you... every school performance, chaperone every field trip or cheer at every soccer game. Tip: take turns going...

  28. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...the host organism being unable to synthesize a nutrient required for growth. Before delivery of the vector...cells are grown in media that supplies the key nutrient. The vector provides the missing synthesis machinery...

  29. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with... 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed plants." Plant cell...

  31. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims...

  33. How to Be an Excellent Trainee

    Type
    Blog Post
    ...than any other one. Luckily, you can do a lot to contribute to the relationship dynamic and set yourself ...notes on general protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and ...

  34. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ... libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled libraries...cells at a concentrated seeding density while centrifuging. Spinfection transduction efficiency can vary...

  35. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...

  36. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...changes to a gene sequence can cause unexpected detrimental results to the protein. Codon optimization could...ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat...

  37. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...start answering questions. r/Science has pretty strict rules about not being too sales-y in posts which...term “implicit bias” which is apparently a troll trigger phrase these days). Redditors ask serious questions...

  38. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...vial of the desired cell line from a reputable distributor such as the American Type Culture Collection ...cloudy. Mycoplasma compete with host cells for nutrients and can alter expression of receptors, ion channels...

  39. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...doing so, they identified the nucleotides that contribute to the instability of Spinach, and were able ...superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing RNA." Nature methods 10.12...

  40. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to...validated antibodies? Many companies that produce and distribute antibodies validate them in a variety of applications...

  41. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were developed...

  43. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...sulfate proteoglycan (HSPG)3 AAV receptor (not strictly required)2 Human fibroblast growth factor ...to Addgenies Brian O’Neill and Ina Ersing for contributing their expertise! References and resources References...

  44. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... activated tools, can control everything from electrical activity to gene expression, and even CRISPR ...2019). Cortical layer–specific critical dynamics triggering perception. Science, 365(6453), eaaw5202. https...

  45. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection...Park, S., Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible...

  46. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory Board member...target sites occur ~1 in every 8 nucleotides, restricting gRNAs to 5 – 65% of the protein coding region...

  47. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia... your time as they can result in spurious yet intriguing findings that fail to replicate. Plan experiments...

  49. Finding Your Science Policy Path

    Type
    Blog Post
    ...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans... challenging if you are trying to apply for an outright job in a field where you have no specific scientific...

  50. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...all know that in the lab there are often little tricks that are essential for experiments but that nobody... not (Figure 4). 5. In theory you could use densitometric analysis to quantitate the intensity of the ...
Showing: 551 - 600 of 884 results