Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 964 results for: app

Showing: 551 - 600 of 964 results

  1. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...What the Future Holds and the Scientists Making It Happen, is an exercise in restraint. The very first subheading... primarily on fluorescent proteins and their applications, tackles much broader issues in this brief volume...ago I would have nodded my head knowingly and appreciatively throughout this chapter. Now, these observations...

  2. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... or burn yourself out trying to slog through unappealing work in your precious free time.  And don’t be...- will be required to produce an outcome you’re happy with. Know if you’ll need specialized equipment ...then pick whichever is most accessible or most appealing to you. And if you’re not sure what all the possibilities...

  3. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...the most important is letting people know it’s happening. Aim for 3-4 weeks advance notice for the first...the Addgene bioinformatics journal club! What happens during journal club? There are many common ways....  These variations are suited for different applications and should be carefully chosen for each group...

  4. An Introduction to Adenovirus

    Type
    Blog Post
    ...which a vaccine is available. However, here it appears that the culprit for these unusual cases was either... the removal of certain adenoviral genes that apparently contribute to virulence (Tollefson et al., 1996...on the Addgene blog Viral Vectors 101: Viral Applications Adenoviral Vector Production and Troubleshooting...

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    ...Addgene  Choosing an expression system Choosing an appropriate expression vector is the first step in generating...binary gateway vectors and can be used for promoter swapping and generating N and C terminally tagged fusion...phenotypes. For most phenotypic analysis it is appropriate to begin working in the T2 generation. The selection...

  6. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ..., Alexa Fluor 568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color...Mouse IgG2a TP1129 pTP1005 (3x cysteines) Mouse Kappa TP1170 pTP1174 (1x cysteine) pTP1172 (3x cysteines...labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. eLife. PubMed...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... an ideal way to deliver AAV to the PNS. It’s a happy accident that AAV.PHP.S targets the PNS since it...jumble of fluorescent neurons. A simple but flawed approach to multi-color labeling is to inject a mix of ...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments...

  8. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which are summarized...which method you choose, make sure to use the appropriate antibiotic for selection.   Lysing bacteria...to determine their size. Make sure to use an appropriate molecular weight standard for reference and to...

  9. CRISPR Antimicrobials

    Type
    Blog Post
    ...virulent or antibiotic resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria...to combat antibiotic resistance using multiple approaches. Major restaurant chains including Chipotle and...bacterial host range. To further potential clinical applications, Ando et al. recently engineered synthetic phages...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...or growth stage). A prey library can either be applied to the bait in a single population (e.g. transforming...although these can often be accounted for using appropriate replicates and controls (see Tips below), and.... Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, C., Ellerbrok, ...

  11. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ..., Zetsche et al. developed a Cpf1 multiplexing approach using a single crRNA array to express up to 4 ...-target editing in a cell culture model. The application of Cpf1 to genome editing is exciting both in...in terms of basic science and translational applications. This discovery of this type V CRISPR system ...

  12. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... result seems to be a balance of productive and happy employees. The teambuilding culture helps us build...throughout the years. This team building merriment happened naturally when Addgene was a family affair back... sure people know that there is something fun happening! Addgene actually has chimes outside of our break...

  13. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...movement in an open field box or a circular corridor apparatus using a camera or tracking software. Locomotion...and might open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents...measuring the time spent in a particular part of the apparatus, it turned out that the IL-1 beta overexpressing...

  14. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...Paul Blainey’s lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi...for microglia research and broader therapeutic applications.  Find microglia-targeting AAV plasmids here...reconstituting coding sequences for a range of applications, including CRISPR activation by dCas9-VPR, prime...

  15. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...highlights some of the latest breakthrough therapeutic approaches. What is Rett Syndrome? Caused by a mutation ...technical challenges to overcome for both of these approaches, such as delivery of the editing machinery to...Extends the Survival of MeCP2-Null Mice without Apparent Toxicity after Intracisternal Delivery. Molecular...

  16. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...chromatic-opening agents to boost expression to appreciable levels. We wanted to create second-generation...ultrasound imaging of nanoscale acoustic biomolecules. Appl. Phys. Lett. 110, 073704 (2017). Hurt, R. C. et ...

  17. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ... process, and request your sequence with the appropriate flanking restriction sites and overhangs already... help you in your MoClo quest, so good luck and happy cloning!   Resources Additional resources on the...Modular Cloning Plasmids 101: Modular Cloning Applications and Kits Plasmids 101: Golden Gate Cloning ...

  18. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...which helps increase cellular uptake, but this approach requires compatible cell lines that express viral...individual analysis. A shuttle vector designed for this approach in a mammalian cell experiment should still include...terms are typically used in different (though overlapping) ways. Broad host range plasmids are those that...

  19. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...used to generate AAV.  One study showed that approximately 50% of viral capsids were genome-containing ...particles can be identified after negative staining and appear darker than full vector particles. This image shows...experiments? Because (physical) AAV titers are approximations and they depend highly on the experimental ...

  20. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ... its reintroduction, inversion and re-inversion happen at the same probability. Continuous inversion of...the same time from two different plasmids this approach becomes problematic as it reduces the probability... Cell Types for Imaging and Long-Range Circuit Mapping.” Journal of Neuroscience 28(28): 7025–30. PubMed...

  21. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...institutions, or institutions they're thinking about applying to, are doing - for example, your benefits may...get rid of postdocs; that current postdoctoral appointments may shorten in length; that postdoc salaries...trainee workforce has clearly not been a sustainable approach to biomedical research in the U.S. and postdoctoral...

  22. The Challenges of Cell Culture

    Type
    Blog Post
    ...especially with industry. Today we also have the appropriate tools to verify our cells lines. Even if you ...the results are reliable. With this two-pronged approach, the many mistakes of the past can be prevented...including GSK, AstraZeneca-MedImmune, and Infors & Applikon. Due to his high level of experience he now travels...

  23. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled...Scarless Modification of Proteobacterial Genomes." Applied and environmental microbiology (2019): AEM-00990...chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015): 5103-5114. PubMed...

  24. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay is quantitative and...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...

  25. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...enjoy a warm meal on a winter day, you should be happy to learn that many DNA-based reagents are named ...aptamer soup by exploring the development and applications of fluorescent RNA aptamers in Addgene’s collection...fluorescence when bound to DFHBI despite being approximately half the length (Warner et al., 2014); its length...

  26. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... explain how to verify that your cells were appropriately edited for your desired mutation - insertion...frequency is 50%, then you could infer that approximately 25% of cells are likely -/-, 50% are -/+, and...sequencing confirmation. All knock-ins A method applicable to knock-ins both large and small is a software...

  27. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... barrier of the endothelium, but they could be applied to study other actin-dependent processes in cells...) and to introduce unnatural head groups for applications like fluorescent labeling via click chemistry...constructs are great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks...

  28. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...can be engineered to bind specific RNA bases. By swapping out these different modular repeat segments, you...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...

  29. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety... publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy...Ruan K, Wang F, Xu G, Hu R (2016) A CRISPR-based approach for targeted DNA demethylation. Cell Discovery...

  30. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...tools Once you’ve selected the class of tool appropriate for your experiment, you’ll need to find a specific...Yizhar, O., & Deisseroth, K. (2012). Principles for applying optogenetic tools derived from direct comparative...

  31. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi...cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi...expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res...

  32. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A....that they could reprogram the genomic target by swapping the homology arms for new targets. And, importantly... is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger...

  33. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...the coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, ...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

  34. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...cross-team guy” at Addgene, an unofficial title that I happily embrace (and admittedly, I may have started that...identify as one.  But some time around 10th grade, I snapped out of the phase of my life that had me listening...you’re part of a team - it will just make you a happier person. What does a good team need? Besides that...

  35. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...have been increasingly popular in fundamental and applied research since their first use in the early 90’... at transferring the gene of interest to the appropriate cells, it was obvious that its safety was a disadvantage...either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express only the ...

  36. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...pool has been sufficiently selected with the appropriate antibiotic so that every cell in the culture ...stable cells such that the next day they will be approximately 50–60% confluent. For 293T cells this is about...confluent or the medium is acidified (i.e., the medium appears orange/yellow if using a standard phenol red pH...counting the cells. When you ultimately transfer approximately 50–100 cells to the conditioned medium, it will...solution at a concentration of 5 cells/mL. Prepare approximately 10 mL of this cell solution for each 96-well...which you see growth. At this time, the cells will appear as colonies in the well ( Figure 1 ). You will ...

  37. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...contains the expected size insert. By selecting the appropriate enzyme(s), one can either linearize a plasmid...interest (see below for recommended amounts) Appropriate restriction enzyme (see manufacturer's instructions...instructions for proper amount) Approrpriate restriction digest buffer (see manufacturer's instructions) Gel...others. When choosing restriction enzymes for this approach, it is often a good idea to choose two different...products on a gel and those clones with the appropriately sized products should have the correct orientation...

  38. Centrifugation

    Type
    Protocol
    ...container Personal protective equipment (PPE) appropriate for the biosafety level of the lab space Reagents... like the one in the picture above. Wear PPE appropriate for the lab space in which you are working. Even...that the centrifuge is clean and that everything appears to be working smoothly. Place your sample tubes... the centrifuge so that the pellets form in approximately the same place in each sample. Place the centrifuge...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

  39. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...chance that all the edits you want to make will happen.  There are many reasons to multiplex gRNAs, some...adjacent to the sites. When digested with the appropriate Type IIS enzyme, the unique flanking overhang...subsequent “bricks” to be added one by one. This approach is especially useful for combinatorial analyses...

  40. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...huge innovation, there was a limitation to this approach. CBEs and ABEs are base transition editors. They...GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing. Instead of fusing ...abasic site. In this post, it refers to the same approach as a CGBE. BER Base Excision Repair A cellular...

  42. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...than longer ones. Thus, you can determine the approximate length of a DNA fragment by running it on an ... bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock... bands will be differentially intense. If this happens, you can just soak the gel in EtBr solution and...Run the gel at 80-150 V until the dye line is approximately 75-80% of the way down the gel. A typical run...are usually referred to as ‘bands’ due to their appearance on the gel. Pro-Tip If you will be purifying ...

  43. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...microcentrifuge. Remove the gel from the plastic wrapper and rinse with deionized water. Gently remove the...5 µL of the prestained protein ladder to the appropriate well. Pro-Tip When possible, skip one lane before...µL of each recombinant antibody sample to the appropriate well. Pro-Tip Leaving clear lanes between samples... Take a brightfield image of the gel with an appropriate imaging system. Figure 1 Recombinant antibody...Gels . Select Plot Lanes . One graph per lane will appear with peaks representing each protein band. Use ...

  44. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

  45. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    ...wintertime festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the New Year. Will your lab be a lucky winner? Happy holidays from all of us at Addgene! We look forward...

  46. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ... Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the New Year. Will your lab be a lucky winner? Happy holidays from all of us at Addgene! We look forward...

  47. Weighing Reagents Protocol

    Type
    Protocol
    ...the basics of how to weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to ...contain the reagent while on the balance, and an appropriately sized container to put the reagent in. For some...container. Dispose of the excess material into an appropriate waste container if the material is biohazardous...biohazard solid waste container. Conclusion Appropriately weighing reagents is important to ensure the...

  48. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ... when Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast...Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon ...

  49. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...Performing In Vivo CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced the very first...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and...

  50. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...
Showing: 551 - 600 of 964 results