...discussing recent publications in a specific field(s). Most anyone who has spent any time in academia has...of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal club ...

...Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose an empty backbone...Use this to choose the antibiotic resistance gene(s) used for selection in bacteria. Has Viral Service...

... https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. Hedrich...

.... PubMed Central PMCID: PMC4142810. 3. Mitalipov, S. and D. Wolf, Totipotency, pluripotency and nuclear...46-53. PubMed PMID: 17989069. 8. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from...

...components, and the accessibility of your target epitope(s). To achieve these goals, researchers commonly use...//doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...

...TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance of NHEJ...still being explored. More broadly, Sakuma et al.’s use of MMEJ represents another strategy researchers...

...constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371...

...Henrici, R. C., Pecen, T. J., Johnston, J. L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular...

...Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent...

...Vanderperre S, Paul R, Björklund M, Ahr D, Ahmed AW, Spinelli L, Brun C, Basler K, Merabet S (2018) Generation...

...identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing while reducing...from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...

...recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even temperature change... all of the droplets, but you can go as low as 1C/s. Increasing the elongation time to 2 minutes and the...

... to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination...doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of Lentiviral...

...References and Resources References Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences...Sciences Education. 15(4):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in...

...You’ll first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to ...Barnett, Jai A. Pathak, Christopher J. Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation...

...Zebrafish (Danio rerio) have been used since the 1930’s in a range of biological studies, including investigations...data in useful ways. Zebrafish mutants In the 1990’s, two revolutionary large-scale random mutagenesis ...

... adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other Ly6 ... these two references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive gene...

... the genome to introduce the genetic modification(s) contained within it by selecting for Zeocin resistance...19416369. Kulakauskas, Saulius, Arvydas Lubys, and S. Dusko Ehrlich. "DNA restriction-modification systems...

...transgenic plants in the process.     References 1. Emami, S., Yee, M. and Dinneny, J. R. (2013) ‘A robust family...24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method of identifying...

... Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome... this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...