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We narrowed to 431 results for: %s

Showing: 221 - 240 of 431 results

  1. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...species of yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used...organism in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

  3. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...by secondary structure. It is thought that poly(A)s are used to control the cellular concentration of ... end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ. An Internal...

  4. AAV Molecular Tools

    Type
    Collection
    ...Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent...

  5. Lentivirus ddPCR Titration

    Type
    Protocol
    ...dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...

  6. Common Injection Routes in Mice

    Type
    Blog Post
    ... https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. Hedrich...

  8. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...components, and the accessibility of your target epitope(s). To achieve these goals, researchers commonly use...//doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...

  9. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...discussing recent publications in a specific field(s). Most anyone who has spent any time in academia has...of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal club ...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):E1152-61 Pubmed PMID 23487772...73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde neuronal tracing...

  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...targeted sequence at the locus of interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but...AT-rich loci or genomes. Cas9s from other species, like S. aureus and N. meningitidis require different PAM ...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance of NHEJ...still being explored. More broadly, Sakuma et al.’s use of MMEJ represents another strategy researchers...

  17. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...You’ll first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to ...Barnett, Jai A. Pathak, Christopher J. Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation...
Showing: 221 - 240 of 431 results