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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 440 results for: %s

Showing: 221 - 240 of 440 results

  1. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Park, K. U., Schwanke, M., Office, E., Schneider, S. R., Abraham, J., Hensley, A., Jones, K. L., & Brzezinski...

  2. Lentivirus ddPCR Titration

    Type
    Protocol
    ...dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...

  3. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...discussing recent publications in a specific field(s). Most anyone who has spent any time in academia has...of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal club ...

  4. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...components, and the accessibility of your target epitope(s). To achieve these goals, researchers commonly use...//doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...

  6. Common Injection Routes in Mice

    Type
    Blog Post
    ... https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. Hedrich...

  9. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):E1152-61 Pubmed PMID 23487772...73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde neuronal tracing...

  10. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...targeted sequence at the locus of interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but...AT-rich loci or genomes. Cas9s from other species, like S. aureus and N. meningitidis require different PAM ...

  11. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance of NHEJ...still being explored. More broadly, Sakuma et al.’s use of MMEJ represents another strategy researchers...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Human Mouse Fly Yeast T. gondii E. coli P. aeruginosa S. pneumoniae M. tuberculosis M. smegmatis Green monkey...136986 Knockout Human Doench 3rd 2 40,964 Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 161769 Inhibition...Pool for Streptococcus pneumoniae 170432 Knockout S. pneumoniae Veening N/A 1 1,499 MusCK Library 174196...

  15. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by optimizing...

  17. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing while reducing...from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...

  20. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright and photostable yellow fluorescent...here! Singh, I., Fernandez-Perez, D., Sanchez, P. S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing...
Showing: 221 - 240 of 440 results