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Showing: 221 - 240 of 430 results

  1. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...species of yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used...organism in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

  3. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...by secondary structure. It is thought that poly(A)s are used to control the cellular concentration of ... end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ. An Internal...

  4. Lentivirus ddPCR Titration

    Type
    Protocol
    ...dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...discussing recent publications in a specific field(s). Most anyone who has spent any time in academia has...of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal club ...

  6. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...components, and the accessibility of your target epitope(s). To achieve these goals, researchers commonly use...//doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...

  7. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...targeted sequence at the locus of interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but...AT-rich loci or genomes. Cas9s from other species, like S. aureus and N. meningitidis require different PAM ...

  8. Common Injection Routes in Mice

    Type
    Blog Post
    ... https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. Hedrich...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):E1152-61 Pubmed PMID 23487772...73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde neuronal tracing...

  13. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance of NHEJ...still being explored. More broadly, Sakuma et al.’s use of MMEJ represents another strategy researchers...

  16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome... this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...

  18. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...References and Resources References Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences...Sciences Education. 15(4):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in...

  20. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing while reducing...from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...
Showing: 221 - 240 of 430 results