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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 503 results for: Ank

Showing: 221 - 240 of 503 results

  2. Typing CRISPR Systems

    Type
    Blog Post
    ...overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3. Cas3 functions...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...

  3. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...essential to test the expression of your opsin. Thankfully the Deisseroth constructs coexpress a fluorescent...your optogenetics experiments. Stay tuned!  Many thanks to our guest blogger Derek Simon! Derek P. Simon...

  4. Adenovirus Guide

    Type
    Guide
    ...and contain multiple heavily-spliced transcripts flanked by two inverted terminal repeats (ITRs). Adenoviral...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293 or HER911...genome packaging. In helper viruses, this signal is flanked by loxP sites so they can be excised. Adenoviral...

  5. CRISPR Guide

    Type
    Guide
    ...large scale edits by integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated.... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby... PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...

  6. Lentiviral Vector Guide

    Type
    Guide
    ... vif , vpr , vpu , and nef for HIV-1). The LTRs flank all of these genes, and anything in between will...contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging capacity Packaging plasmid...replication-competent lentiviral vectors is very low thanks to the design of second- and third-generation lentiviral...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... with customizable N- and C-terminal modules   Thanks to the lab of Tomás Santalucía, you can now take...because they are transported back to the nucleus (thanks to a NLS on these proteins). The RFP proteins, ...Biol. 2015. PubMed PMID: 26000751   Thank you for reading. You can find all of our Hot Plasmid...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Rivest, A.J., Jung, H.-Y., Kitamura, T., Suh, J., Frank, D., Kajikawa, K., Mise, N., Obata, Y., Wickersham..., W., Zhou, S., Beyer, J., Forsayeth, J., and Bankiewicz, K.S. (2013). Axonal transport of adeno-associated...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...from Cynthia Arokiaraj. Addgene would also like to thank Viviana Gradinaru and Tim Shay for their support...Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith, J., Ibrahim, L. A., Mastro, K. J...

  18. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...recognizing our incredible depositors and requestors. Thank you all so much for an amazing twenty years of sharing...

  19. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...helpful resources for their CRISPR experiments.  Many thanks to:  Daniel Mejías Sánchez, PhD, translator  Denise...
Showing: 221 - 240 of 503 results