We narrowed to 499 results for: Ank;
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TypeBlog Post...overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3. Cas3 functions...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...
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Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...and a donor DNA that contains the tag sequence flanked by homology arms (Figure 1). In the first step ...PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift in the... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post... Cre/lox recombination system. A researcher can flank fluorescent proteins in a plasmid with loxP sites...Immune response dynamics are much better understood thanks to these technologies but many questions remain... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...essential to test the expression of your opsin. Thankfully the Deisseroth constructs coexpress a fluorescent...your optogenetics experiments. Stay tuned! Many thanks to our guest blogger Derek Simon! Derek P. Simon... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post.... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...you reach out to experienced legal counsel. Many thanks to our guest bloggers Lauren Celano of Propel Careers...healthcare IT, boutique consulting, investment banking and venture capital areas. We place individuals... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...should we use all tools as some kind of a meta/Frankenstein app? Or perhaps there is a clear winner, is ...communication between developers and end users. Thanks to our guest blogger Cameron MacPherson! Cameron... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post... with customizable N- and C-terminal modules Thanks to the lab of Tomás Santalucía, you can now take...because they are transported back to the nucleus (thanks to a NLS on these proteins). The RFP proteins, ...Biol. 2015. PubMed PMID: 26000751 Thank you for reading. You can find all of our Hot Plasmid... -
Using AAV for Neuronal Tracing
TypeBlog Post...Rivest, A.J., Jung, H.-Y., Kitamura, T., Suh, J., Frank, D., Kajikawa, K., Mise, N., Obata, Y., Wickersham..., W., Zhou, S., Beyer, J., Forsayeth, J., and Bankiewicz, K.S. (2013). Axonal transport of adeno-associated... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...from Cynthia Arokiaraj. Addgene would also like to thank Viviana Gradinaru and Tim Shay for their support...Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith, J., Ibrahim, L. A., Mastro, K. J... -
New Antibodies in the Lab Animation!
TypeBlog Post...refresher on — these common antibody applications. Thanks for watching, and we hope you found the video radd-ically... -
Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog Post...the segment on CRISPR tools for plants. Special thanks to our sound engineer/producer, Eddy Page! Edward... -
Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!
TypeBlog Post...Listen to the Segement on the pPSU Plasmids Special thanks to our sound engineer/producer, Eddy Page! Edward... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post...Blugene's new virus friend finally has a name! We thank you for your participation in suggesting names and... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog Post...protein. Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page! Edward... -
Addgene's 20th Anniversary Party
TypeBlog Post...recognizing our incredible depositors and requestors. Thank you all so much for an amazing twenty years of sharing... -
CRISPR 101 eBook...en español
TypeBlog Post...helpful resources for their CRISPR experiments. Many thanks to: Daniel Mejías Sánchez, PhD, translator Denise... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post...: Atharva Sahasrabudhe; Polina Aniveeka A big thank you to all who have contributed to the Addgene blog... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post...Subscribe to the blog to stay in the know! No pranks, just fun and games this April Fool’s Day on the... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog Post...topper, tube “lights,” and autoclave tape garland. Thanks @ribosholmes! Frogs and tacos The Miller Lab...