We narrowed to 9 results for: Ank;

...Genome Engineering, CRISPR, Bacterial Expression Anke Becker Plasmids to perform genome engineering in...with an overhang of 4 bp. If each DNA module is flanked with a standard overhang sequence at the restriction...promoters, UTRs, coding sequences, terminators, etc.) flanked by fusion sites into individual Level 0 plasmids...

... packaging genes gag , pol , and env . The LTRs flank all of these required genes, and anything in between...contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging capacity Packaging plasmid... vectors are useful research and clinical tools thanks to their long-term expression of a transgene. Stable...generation of large-scale, high-titer preps. This is thanks to established retroviral production cell lines...-competent gamma-retroviral vectors is very low thanks to the design of the plasmids. The genes used for...

...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...which controls for your antibody’s specificity. A blank (contains water or buffer instead of a biological...Visualization methods should employ positive, negative, and blank controls. Antibody Applications - Cell Sorting Methods...with only one antibody, for each antibody used. Blank controls in the form of sample buffer must also ...

...and contain multiple heavily-spliced transcripts flanked by two inverted terminal repeats (ITRs). Adenoviral...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293 or HER911...genome packaging. In helper viruses, this signal is flanked by loxP sites so they can be excised. Adenoviral...

...large scale edits by integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated.... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby... PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...

... vif , vpr , vpu , and nef for HIV-1). The LTRs flank all of these genes, and anything in between will...contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging capacity Packaging plasmid...replication-competent lentiviral vectors is very low thanks to the design of second- and third-generation lentiviral...

... The entry clone now has recombined attL sites flanking your DNA fragment of interest. Now that you have...

...selection marker. Plasmid delivery is versatile thanks to the wide range of options for the Cas enzyme...

...consists of two open reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats (ITRs)....