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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 709 results for: BASE;

Showing: 221 - 240 of 709 results

  1. Plasmids 101: Protein tags

    Type
    Blog Post
    ...Though there are a number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase...DYKDDDK 1 Affinity and Purification Good for antibody-based purification; has inherent enterokinase cleavage...residues improves solubility; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability...1.4 Affinity and Purification Good for antibody-based purification Combo and cleavage tags Frequently...

  2. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...names of the plasmids you plan to use Enter the base pair size of those plasmids Enter plasmid concentration...plasmid needed per dish or stack. Calculations are based on using 28 μg of total plasmid (Transfer, helper...

  3. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...Since the number of base pairs for each varies, it is difficult to calculate this based on DNA concentration...restriction sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends of...

  4. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies that...Nano-Lanterns to other cellular proteins. BRET-based biosensors utilizing NanoLuc® luciferase Many intracellular...powerful the FRET signal. In well-designed FRET-based biosensors, the intensity of the FRET signal can...Schmid, J.L., et al. (2016) CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent...) Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing. Nature Comm. 7...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...postsynaptic receptors and transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR...The Abudayyeh-Gootenberg Lab developed this tool based on several new site-specific retrotransposon elements...can make a wide variety of genomic edits — single-base changes, small deletions, and large insertions up...insertion is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids here! Fell, C...primates, and human tissue. Figure 6: Enhancer-based targeting of PV interneurons in mice. A) AAV expression...

  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...glycosyl groups, and other modifications to their bases in order to protect their DNA from host cell nucleases...proteins in the (all-too-common) event that homology-based searches return no candidates. Our strategy: Express...in the presence of certain modifications to DNA bases.  Ligate and transform your resultant plasmid libraries...Adapting drug approval pathways for bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016)...Raleigh. "Biosynthesis and Function of Modified Bases in Bacteria and Their Viruses." Chemical Reviews...

  7. Fluorescent Protein Guide

    Type
    Collection
    ...backbones. Fluorescent Protein Kits cpFRET Kit: FRET based biosensors - Pertz Fluorescent Protein Fusion Construct...Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed ...Galleries from Michael Davidson and FSU Spectra Database hosted at the University of Arizona Zeiss Education...

  8. AAV Viral Preps

    Type
    Collection
    ...CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples ...

  9. Video Library

    Type
    Protocol
    ... allows you to isolate and purify DNA fragments based on size. Following gel electrophoresis, you can ...molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA from an Agarose Gel Protocol...

  10. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...you through both experimental and primer design based on fragment sequences and the polymerase used in...joining of multiple mutations spread over thousands of base pairs. Cloning sgRNAs Generating constructs to produce...Cloning Gibson Assembly and Other Long-Homology Based Cloning Methods Screen Plasmids by Colony PCR Addtional...

  11. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...plasmids and those without, we adjust our estimates based on the journal that published the articles.  That...Addgene plasmids as if they comprised 10 percent. Based on this new re-weighting, we can compare citations...Addgene is achieving its goal of making plasmid-based science more accessible…and accruing more citations..., we went to one of the largest bibliographic databases available, Web of Science. For each publication...number of citations the paper receives. From the database we pulled the journals from 2005 to 2015 in areas...

  12. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...plasmids used to generate these antibodies were based off of a large collection of mouse monoclonal antibodies...Antibodies?  One of the benefits of using a plasmid based system for expression of R-mAbs is that it can easily...Additional Resources on the Addgene blog:  Plasmid-Based Recombinant Antibodies  Plasmids 101: Secondary ...

  13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...genomic SDM relatively straightforward, but plasmid-based SDM has lagged behind. While commercial kits are...PCR is used to splice together two PCR amplicons based on sequence homology, and the subsequent product...primers also have some homology to each other; ~17 bases is optimal. After being treated with DpnI to digest...

  14. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...limited to neuroscience. In fact, precise light-based control of biological processes is highly useful...better fine-tune the level of output gene expression based on the input light intensity. In addition to these...strains of E. coli, as well as with other plasmid-based systems, which will enable the assembly of larger...

  15. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...a DNA-binding domain to recognize any desired 15 base pair sequence in the genome in rapid fashion. The...All you do to create a protein that binds those 15 base pairs is take four plasmids out of the library in...genome-editing system for generating human stem cell-based disease models." Cell Stem Cell. 12, 238-251 (7 ...

  16. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...and what work he's planning on doing with CRISPR-based gene drives focusing on safety and the ability to...Board with a particular interest in generating Cas9 based tools to enable facile control of DNA and RNA.   ...

  17. Plasmid Collections

    Type
    Collection
    ...Resources Our curated resources group plasmids together based on type or application. Browse our guides to find...

  18. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

  19. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...degradation. This degradation can continue for many kilobases in one or both directions from the target region...
Showing: 221 - 240 of 709 results