We narrowed to 658 results for: CHI;

...donated to ABRC by the Arabidopsis research and teaching community through the TRAINED initiative (Translating...ordering from ABRC for the first time, we recommend reaching out to us at [email protected] for help setting ...professor? Whether you are looking to develop a new teaching resource or you want to introduce Arabidopsis ...

...including a mouse monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide...still the most prevalent clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with...Addgene’s monoclonal antibodies (mouse, rabbit, and chicken) to this tag! GFP  GFP is more than a peptide –...

...a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared to ...dCas9 fused to cytidine deaminases like APOBEC. To achieve this they created a lentiviral vector system that...

...PMCID: PMC1209583. Watanabe, Tsutomu, Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF DRUG...and Esther M. Lederberg. “Sex Compatibility in Escherichia Coli.” Genetics 37.6 (1952): 720–730. PubMed ...

...guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various...  Simply input these parameters and you’ll find matching FP suggestions. If you’re not sure exactly what...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

...their structure or their interaction with AAVs (Schiller et al., 2018). Exosomal vesicles secreted from...behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs have improved transduction profiles...PMID: 29296848. PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of...

... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...

...Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth... at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, ...Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol...

...samples, the rest will be put into a status we call “Archived.” This status allows us to store plasmids and ...These new processes have been instrumental in achieving our mission to help science move faster in the...Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular Stomatitis Virus...

...brightness, Less signal distortion from photoswitching or photobleaching Improved signal-to-noise ratio (SNR...% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute...

... luciferase reporters aren’t susceptible to photobleaching — they’ll keep emitting light as long as their...Sensitivity Higher Lower Background Lower Higher Photobleaching/phototoxicity Not susceptible Susceptible ...advantages of luminescent assays (low background, no photobleaching) but provides flexibility by pairing the luciferase...

...can be affected by both autofluorescence and photobleaching. Researchers have long been interested in using... cells. As Nano-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple...applications. For long-term imaging, especially where photobleaching and phototoxicity are a concern, these constructs...

...instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor...PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing constructs, transcript...

...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...

...GFP. The lab tested the base editors in vivo and achieved therapeutically relevant editing efficiencies ...heart, and skeletal muscle. The dosing required to achieve these efficiencies was also between 1013 - 1014...Ace absorbance increases and results in higher quenching of mScarlet emission. Figure 2: Ace-mScarlet...

... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science...position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “There were jobs...applied and I got it.” Science writing at Fred Hutchinson Cancer Research Center Work as a Science Writer...

...what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward from this...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing...’s important to remember that the study hasn’t achieved any end goal not available with current techniques...

...fluorescent protein tags. Tamura et al. Commun Biol. 2021 Daichi Kamiyama Cerulean Cerulean(1–10) for use with...fluorescent protein tags. Tamura et al. Commun Biol. 2021 Daichi Kamiyama ECFP C155 of ECFP pairs with N173 ...fluorescent protein tags. Tamura et al. Commun Biol. 2021 Daichi Kamiyama sfCherry2 sfCherry2(1–10) and sfCherry2...

...intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting time and money on optimizing...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...

...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...