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We narrowed to 658 results for: EPO;

Showing: 221 - 240 of 658 results

  1. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...community. This is no small task, however, at a repository that consistently receives around 200 new DNA...data on every plasmid we distribute through our deposit process, and then we carefully track samples through...your effort. In addition, if you should choose to deposit your plasmids with us, it will be easy to find ...

  2. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research investigator in the...to EGFR inhibitor (Hata et al., 2016).  Bhang deposited the library with Addgene because she wanted to...sharing protocols one by one,” and she is excited to deposit the library with Addgene because it is a “really...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...vector blog posts for updates on the most recent deposits, protocols, and tips for using retroviral vectors...Plasmid #17408)   This is a lentiviral vector deposited by Dr. Tyler Jacks. It carries CRE recombinase...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

  5. Lab Automation at Addgene

    Type
    Blog Post
    ...With our ever growing rate of plasmid deposits (over 100 deposits per month!), manual transformations were...Management Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources...

  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...recently contributed a set of plasmids to the repository that will help epigenetic researchers custom ...

  8. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...scientists who were potential Addgene requestors and depositors. China is in one of the top 10 countries in number...was kicked off by Nobel Laureate (and Addgene depositor) Craig Mello. Taking his groundbreaking work in... labs are using CRISPR/cas9 and more are being reported. Zhou Songyang from Baylor College of Medicine...

  9. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...As the Chair of the Advocacy Committee, I had to report to the Board of Directors and liaise with the Executive...accordingly. For example, the quarterly and annual reports I had to write on behalf of the Advocacy Committee...to overcome the personal frustration and submit reports in the format requested which in the long run was...

  11. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...magnetic fields (Frank Hayward et al., 2024). They deposited their plasmid with Addgene, hoping to inspire .... Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...reproduced their EGFP findings since the initial report in 2023.)     Figure 1: Fluorescence intensity...

  12. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...the sequencing results of the plasmids in our repository with known reference sequences, such as full ...plasmid sequences provided by the laboratories that deposit their plasmids with us or other entries in NCBI...NCBI’s numerous databases. As our repository has grown over the years (we now have over 60,000 plasmids!...

  13. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...pathway is ubiquitous, with successful recombination reported in all laboratory E. coli strains tested so far...use of this pathway as a cloning tool was first reported over 25 years ago (Douglas and Howard, 1991, Bubeck...exploiting bacterial in vivo assembly." Scientific reports 6 (2016): 27459. PubMed PMID: 27264908. PubMed ...

  14. MXS Chaining

    Type
    Blog Post
    ...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...

  15. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers...designed a tool that could analyze CRISPR sgRNAs and report the likelihood of any given sgRNA having an ancestral...complicating the issue is the long and ugly history of reporting false essentialist links between biology and ancestry...

  16. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be...t common for simple transfection experiments. Reporters Finally, in some cases a researcher may choose...

  17. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse...substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with Addgene...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids...

  18. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...primary antibody respectively. Avidin bound to a reporter is then added to the reaction for easy detection... experiments. During detection, a biotinylated reporter enzyme that is pre-incubated with free avidin ...protein of interest leading to amplification of the reporter signal and easier detection. This strategy is ...

  19. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ... Team is responsible for guiding new deposits into the repository, performing quality control, and providing...kit creation and quality control efforts with depositors; Gizela, the lab member who knows how the physical...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...and were suggested to be the best gRNAs by the depositing lab. The different labs use different algorithms... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ...
Showing: 221 - 240 of 658 results