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...Plasmid Collections Depositor Plasmid Collections Depositor Collections Addgene's repository contains 167,150...collections from depositors. To help you find what you're looking for, we've gathered our depositor collections...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression...

... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...for gene expression assays and bioluminescent reporters. Plasmid...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...-lives, making them useful as transcriptional reporters, but are less-than-ideal protein tags due to their...or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids are meant...luciferase and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions...

...68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid...Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 ...Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO...EcAR7 Plasmid 52054 SepOTSµ ( aka. B40 OTS) Strain 34929 BL21ΔserB Plasmid 34624 SepOTSα tRNA-Sep ( aka. ... Depositor Collections Rinehart Lab Phosphoprotein Reagents...#188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important technical note:...optimized phosphoserine orthogonal translation system, SepOTSλ (Plasmid #68292) , they were able to demonstrate...

...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Neurotransmitters Cell Physiology Browse All Other Reporters Additional Resources Fluorescent biosensors are...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...Calcium Ratiometric calcium biosensors from nested reporters of green- and orange-emitting proteins Ratiometric...

...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent ...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...it. Bacterial one-hybrid (B1H) systems also use reporter genes to identify interactions between proteins...

... and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter assays use light produced...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing... Depositor Collections Promega Plasmid Collection Promega... workflows. The collection contains luciferase reporter vectors for sensitive gene expression analysis...collection. For example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase"... a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa)...Addgene Find additional information about luciferase reporter assays (Link opens in a new window) from Promega...

...Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository, including...in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in a new window...largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository Tissue...MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (Link opens in a new window) PMID: 35148843...window) Jackson Labs - mouse line developer and repository, and CRO for centralized behavior scoring (Link... purposeful hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens...contributing your plasmids! To get started with your deposit, please click here ....

...Surface Display cDNA Depositor Collections A modular set of plasmids from a depositor organized by a common...Collections Addgene Plasmid Collections Addgene's repository contains 167,150 plasmids . We've gathered our...our curated plasmid resources, kits, and depositor collections on one page to help you find what you're...looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources group...common use. Click here to view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein...community that has established a common place for deposited plasmids. ASAP Discovery Consortium ARF GTPase...Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific disease research areas. ...

...the community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse ...available at Addgene. Browse featured collections or depositing labs. Plasmid...Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks JBEI - Joint Bio-Energy...

...SynBio Genome Editing Gene Silencing Reporters Addgene’s repository contains many plasmid tools that were...Return to top Reporter Plasmids The table below highlights a selection of popular reporter plasmids that...plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene silencing, and more...from genome engineering and gene expression to reporter systems. Addgene’s collection includes plasmids...The following plasmid kits contain a variety of reporter plasmids for the study of protein–protein interactions... Golden Gate (MoClo) Benjamin Field Additional Reporter Plasmid Resources Visit our Fluorescent Protein...

...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-...44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection. Dapeng...

...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more...page for more information Genome Modifications, Reporter Assays, mRNA Regulation, and More Find empty backbones...backbones for other uses such as genome modification, reporter assays, shRNA expression, transgenics, and more...TALEN array for genomic engineering Luciferase Reporter pGL3 Basic Vector - Firefly luciferase vector...genes can be inserted into this firefly luciferase reporter to test for their effects on protein production...

... aren't in the repository? Help us widen our resources for the community by depositing your useful tools...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...bacteria, and more. Synthetic Biology : Browse our deposited tools and resources, or read our guide on assembly... reference page for CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast...Genome Engineering blog post to see how Addgene depositors are making this possible in a range of organisms...

...The Eric Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based system to add a tag...homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas tagging system were... Tagging System Recently, Yannick Doyon's lab deposited a plasmid which introduces an N- or C- terminal...PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids for CRISPR-based knock-in...microhomologous sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ...

... Depositor Collections Bikard Lab - CRISPR Repression Collection...David Bikard Lab at the Institute Pasteur has deposited a collection of CRISPR arrays to modulate the ...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...

...Addgene's deposit agreements allow Addgene to distribute plasmids on behalf of the depositing institutions...proteins that are structurally characterized (PDB-deposited), disulfide-free, mesophilic, and designed to ...legal agreement between the recipient and the depositing institution(s). It governs the transfer of biological...entity is qualified to receive materials from our repository. All purchases are subject to Addgene's Terms...

...Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core... Depositor Collections...window) of the Baylor College of Medicine have deposited a series of lentiviral and adenoviral transfer...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes...

...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT...

...drives expression of RFP as a reporter. See plasmid 17618 for GFP reporter. Linzhao Cheng 17452 pLenti ...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan...86849 pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin selection. ...

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...following the amplification protocol specified by the depositing lab. After amplification, you’ll need to use ... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols Video protocol for genomic deletions...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...