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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13 results for: EPO;

Showing: 1 - 13 of 13 results

  1. CRISPR Guide

    Type
    Guide
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...following the amplification protocol specified by the depositing lab. After amplification, you’ll need to use ... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols JOVE video reprint: Protocol for genomic...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...

  2. Optogenetics Guide

    Type
    Guide
    ...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators...optogenetics tool - they typically allow the fast depolarization of neurons upon exposure to light through direct... trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic toolbox has been...light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements include...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ... to amplify the library, please refer to the depositor’s protocol for the best results. For some libraries...die or do not pass selection (in the case of a reporter) Sort “winning” cells Sequence those cells that...Resources Science Exchange Scientist (formally Assay Depot) Resources Find additional resources on our website...

  4. Sequencing Primers

    Type
    Guide
    ...that Addgene uses for sequence verification of deposited plasmids....distribute primers. For sequencing plasmids in our repository, we've chosen primers based on the plasmid backbone...of primers for sanger sequence verification of deposited plasmids. Below is a list of commonly used primers...

  5. Modular Cloning Guide

    Type
    Guide
    ...about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit and ...Plasmids with control elements and fluorescent reporters to be used in combination with the MoClo-YTK toolkit...

  6. Chemogenetics Guide

    Type
    Guide
    ...control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...Some retroviral transfer plasmids that have been deposited with Addgene are self-inactivating (SIN). These...

  8. Plan Your Experiment

    Type
    Guide
    ...in question (see CRISPR protocols from Addgene depositors ). Browse empty gRNA expression vectors . Select...facilitates CRISPR/Cas9-based genome editing. Scientific Reports , 5 , 11315. https://doi.org/10.1038/srep11315 ...

  9. Lentiviral Vector Guide

    Type
    Guide
    ...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...the lentiviral transfer plasmids that have been deposited with Addgene are self-inactivating (SIN) plasmids...

  10. Molecular Biology Reference

    Type
    Guide
    ...information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying the function of genetic... genetic elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers...

  11. Educational Resources

    Type
    Guide
    ... How-to Blog Read about plasmids in Addgene’s repository , and related molecular biology techniques. Topics...

  12. Adenovirus Guide

    Type
    Guide
    ...Adenovirus Vector as a Zika virus vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020...

  13. Antibody Guide

    Type
    Guide
    ...emissions. Cells can also be sorted (separated and deposited into tubes or plate wells) by fluorescence activated...
Showing: 1 - 13 of 13 results