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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 639 results for: ESP

Showing: 221 - 240 of 639 results

  1. Let There Be LITE Plasmids

    Type
    Blog Post
    ...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...occurs within minutes of light exposure. Third, the response can be graded with light dose and is fully reversible...

  2. Editor's Choice, August 2016

    Type
    Blog Post
    ...you’d be amazed by the number of out of office responses I’ve received). Clearly we have all of our writers...few successes have trickled into the survey. In response to a bit of a Twitter storm surrounding NgAgo,...

  3. Science as Art

    Type
    Blog Post
    ... science as an art form taps into an emotional response within the viewer, which could promote conservation...that we know very little about these specimens - especially those which are now extinct. A collection of ...

  5. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...online as one part of their job search. This is especially true of scientists seeking a first job after ...hiring managers to take next steps and follow up especially in large companies. It is fine to address the...

  7. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...plastic is a substantial portion of lab waste. Despite the fact that researchers represent a minority ... already leading the cultural change toward responsibility in our lab’s environmental impact, but we can...

  8. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...researchers around the world. We take this responsibility seriously. Every decision we make, every project... in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there ...

  9. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...protein that undergoes a conformational change in response to the molecule it detects. This change then generates...with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction to fluorescent...

  10. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...often driven by the AAV p5 or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small...gene which differ between the AAV serotypes are responsible for serotype-specific differences in tissue and...

  12. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...during storage over prolonged periods of time, especially at room temperature or at 4°C. Storing DNA: temperature...

  14. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...efficiency is consistently higher than that of H840A, especially with smaller nick distances. Using D10A, they...strategy can expand the targetable region, which is especially useful if there are no available guides close...

  15. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...epigenetic effectors. The diversity of tools is especially exciting, including histone acetylation by p300...and Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional...

  16. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...This terrible disease causes the patient to, in response to tissue damage, have excessive bone growth until...patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family...

  17. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...corrective feedback. A manager must be able to respond to the employee’s level of discomfort appropriately...with the manager as with the employee. This is especially true if the problems are large enough that the...

  19. Advice for Choosing a Research Project

    Type
    Blog Post
    ...want to choose a research question that people (especially you!) care about. Have you critically evaluated...have done recently that has been a big hit, and especially look at the new tools and methods that have been...
Showing: 221 - 240 of 639 results