..., blue, and green channels overlaid on a bright-field image. Image from Ma et al, 2016. Using dCas9... the signal to noise ratio of the system, they modified GFP-dCas9 and the gRNA scaffold to enhance complex... with a number of limitations. Since color is specified by PAM sequence, each color requires a different...

...identifying protein-protein interactions, but RaPID identifies RNA-protein interactions. Both methods rely on... not well characterized RNA motifs. This bait identified a new interaction: binding of the RC2H1 protein...what host proteins interact with Zika’s UTR and identified QKI, an RNA-binding protein that’s highly expressed...

...tested this system in vitro and in vivo. They identified cells from 19 genera across four phyla that have...conjugation in vivo studies. However, they only identified seven genera in in vitro experiments, possibly...Clostridium difficile. As mRNA expression cannot be identified with DNA-based qPCR methods, the ability to detect...

...To solve this problem, gRNAs can be chemically modified to resist degradation while still effectively ...crRNA molecule (usually 1-3 of the first 3 bps are modified) for optimal stability and efficacy.  Base modifications...Hendel, A., Bak, R., Clark, J. et al. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in...

...activation or gene editing. The more recently identified Cas12f systems from uncultivated archaea are ...almost 40%. The activity of Cas12f1 with these modified gRNAs is comparable to that of SpCas9. The group...group also confirmed that the Cas12f1 and modified gRNA were effective at genome editing when delivered...

...finding solutions to some of the problems that were identified last year.  In particular, at the symposium we...How many graduates and postdocs – and from what fields – are needed to adequately supply these demands...the number of graduate students accepted in STEM fields (6). Melanie Sinche is a STEM career counselor...

...recombinase can be expressed only in specified cells or at specified times. Combining this with some of ...well-established research tool, especially in the field of mouse transgenics. A few of the most common uses...

...website, the new version of which will feature a simplified user interface, expanded numbers of curated molecules...expanded set of links to datasets and reagents in the field. Additional information can be found in the Datasets...The great progress that has been made in the NR field over the last thirty years or so would not have ...

...restriction enzymes (e.g. BsaI), which cut at a specified distance from their recognition sequence. This... end will now look like this: Note: Elute your purified digestion and PCR products with sterile water ...purification . Step 5: Create Insert Overhangs Treat your purified PCR product with T4 Pol in the presence of dCTP...

...Lacy In 2022, Atsushi Miyawaki and colleagues identified a highly bright and stable fluorescent protein...lab generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which...brains.  Systemic gene delivery capsids previously identified in rodents have not been widely successful in...

...delivering shRNA or miRNA. The ever expanding CRISPR field makes wide use of retroviral delivery systems, especially... recombination events and is widely used in the field of mouse transgenics. Experimental uses include:... most recent developments in the genome editing field. Lentiviral Plasmid Kit Multiple Lentiviral Expression...

...plasmids all have a PR-T4 vector backbone, which is a modified version of L440. This vector is versatile in that...using synthetically made RNA (riboprobes) with modified nucleotides (for example, Digoxigenin-labelled... to purify the dsRNA from bacteria and mix the purified dsRNA with liver paste, and feed the mixture to...

...interactors purified via streptavidin pulldown. Following trypsin digest, PPIs are identified via mass spec...). A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian...

...the need for open science and collaboration. The field already has comprehensive reference pages and curated... resources and tools. That message was fully exemplified in Cori Bargmann’s keynote speech and in the ...elegans (Zhang et al., 2015). In this system a modified TIR-1 protein can degrade degron-tagged proteins...

... Chemogenetics has become a popular tool in the field as it provides a noninvasive method for manipulating...target activation by ligands. PSAMs themselves are modified ligand binding domains. When coupled to the ion...for clinical use. Varenicline has been further modified to create ultrapotent PSEMS (uPSEMs) that have...

...technical and transferable skills that make you qualified for the position. Highlight the outcomes or deliverables...required to give a job talk for a remote interview. Fielding questions can be hard during this talk since you... have made it to the interview if you weren’t qualified for the position. So prepare for the interview...

... deplete, and visualize proteins of interest (Greenfield, 2014). Traditionally, they have been made as... from a protein sample (for instance, affinity purified antibodies from a patient’s blood), scientists...8:76 . https://doi.org/10.1186/1741-7007-8-76 Greenfield, E.A. (Eds.), Antibodies: a Laboratory Manual...

... has been a breakthrough for the genome-editing field and the greater scientific community. In 2014, we...organoids. This work, and many others, pushed the field forward to where it is today. Below we will review...of using the patient’s own cells that have been modified ex vivo, previously edited healthy donor cells...

...both Cre and FLP recombinases.   Figure 1 (modified from Schnutgen et al. Nat Biotech 2003): Basic... turn off one gene and activate another. In the field of mouse genetics, FLEx is currently being used ...targeted neuronal population.    Figure 2 (modified from Schnutgen et al. Nat Biotech 2003): In the...

...12 different capsids, or serotypes, have been identified in nature, while many more have been engineered...tissues. Created with BioRender.com. Beyond the identified serotypes, AAV also has psuedotypes. Psuedotyping...., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., Maguire, C. A., & György, B. (2019). ...