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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 472 results for: FLU;

Showing: 221 - 240 of 472 results

  1. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ... often fall short with the generation of various flux imbalances. The conventional approach has typically... the authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives of...strategy can be applied to effectively change metabolic flux. The dCas9 protein and orthogonal variants can...

  2. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell...second expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ... light responsive than fluorescent proteins required to get a good fluorescent signal. It may not be necessary...are often necessary to visualize expression of fluorescent proteins, for example, as many viral particles...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...bodies decorated with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed... body dynamics. Second, we showed that these fluorescent R bodies can rupture E. coli spheroplasts (cells...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ...27. See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders...

  7. Hot Plasmids - May 2022

    Type
    Blog Post
    ...application. Each visual barcode consists of a fluorescent protein coupled to a specific subcellular localization...different localization peptides with four different fluorescent proteins, and inserting each visual barcode into...and reporters that drive the expression of the fluorescent protein by a specific transcription response ...

  8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...protein. OptoNBs were also tagged with an infrared fluorescent protein tag which let the team detect its co-...revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead, suggesting that OptoNBs...OptoNB-SOS activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These ...

  9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...number in a cell. One can attempt to eliminate fluctuations due to copy number by introducing genes of interest...output. The input, plasmid copy number, directly influences gene expression. By itself, increasing the copy...stabilized promoters perform during these types of fluctuations. When cells are dividing rapidly, multiple rounds...

  11. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...should appear fluorescent if they were infected. While there may be some low-level fluorescent protein or...virus. Alternatively, if the virus encodes a fluorescent protein, the packaging cells could be examined...

  12. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...is particularly useful when vectors carrying fluorescent markers are used as it eliminates the need for...for antibody staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes... lentivirus Volume and transduction time will influence lentiviral titers as will the target cell type...

  13. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...co-express your gene along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow...co-express your gene of interest along with a fluorescent protein or selectable marker, it is easiest to...genes at equivalent levels upstream of an IRES fluorescent reporter for easy detection.5  Just released:...

  14. Retrograde AAV viral preps

    Type
    Collection
    ... pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor... pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine... pAAV-hSyn-GRAB_NE1h Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine...

  15. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...Zimmer, whose previous books focused primarily on fluorescent proteins and their applications, tackles much...middle-aged scientists, the identification of Green Fluorescent Protein (GFP) and its development into an essential...reading this review--in the current period of rapid flux, the chapter might still feel slightly dated by ...

  16. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...are easy to trace and not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color...constitutively express a red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral...morphology studies when used as part of a two part fluorescent tag-then-express system that allows for controlled...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    ...slow-growing protein fiber in the cytoplasm and can be fluorescently labeled. Using lentiviral vectors to stably ...different promoters, specific time points appear as fluorescent bands in the fiber, resembling a “ticker tape... treated with a phorbol ester activator gave fluorescent signal in the ticker tape from cFos::GFP-iPAK4...

  18. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but for simplicity we ...antigen in the sample. A variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available...read on a standard plate reader as opposed to chemifluorescent, and chemiluminescent methods that require...

  19. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used...without signs of toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin...plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%-confluent well of HEK cells in a 6-well dish and change ...

  20. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...successfully and expressing the protein. For example, fluorescence microscopy images resulting from our experiment...was not successful due to the absence of GFP fluorescence from the living, viable cells. This result presents...produces the expected effect, which is green fluorescent cells (Figure 3).   Figure 3: Expression of Plasmid...
Showing: 221 - 240 of 472 results