We narrowed to 342 results for: GAI

1. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ... a kinases in a body part of choice. Zhou et al again used the Raf-MEK-ERK signaling pathway to demonstrate...

2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   Blog Post

   ...constantly communicating with a tumor, proving once again that a tumor is always interacting with its surroundings...

3. Celebrating 15 Years of Scientific Sharing

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   Blog Post

   ...here at our new location to grow. We are hiring again and getting ready to embark on new exciting projects...

4. Summer SciComm Series: Cognitive Load

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   Blog Post

   ...you!)  Amount of information and level of detail Again, this is an obvious one on the surface, but it’s...

5. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...mouse cortex. Image from He Tian. Congrats again to all the recipients! ...

6. Antibodies 101: Polyclonal Antibodies

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   Blog Post

   ...full-length protein that you want to generate antibodies against, or a unique peptide sequence derived from this...

7. Addgene: The First Twenty Years

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   Blog Post

   ...in r/molecularbiology.   Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production...

8. To Each HIS Own

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   Blog Post

   ...from a complex mix using affinity chromatography against an amino acid sequence, or tag, on the protein....

9. All in a Twist: dsRNA

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   Blog Post

   ...found (typically RNA that the RNAi is designed against), RISC can repress translation and/or degrade target...

10. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post

    ...of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the Addgene Blog Learn How...

11. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ...enable you to quickly BLAST your sequencing result against the expected sequence for quick verification, which...

12. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    ...tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells with GFP, at a ...

13. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent adenovirus...

14. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ... who participate in the course show significant gains in both content knowledge and research skills (8...

15. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...with an antibody. This can artificially select against PPIs that aren't stable outside the native cell...

16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    ...transduction; but these methods aren’t stealthy enough to gain access to some cells and tissues, such as human ...

17. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post

    ...the 22nd International C. elegans meeting, I was again reminded of the extent that C. elegans researchers...

18. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post

    ...multiple genes to generate a cellular phenotype. Again ABE8.20-m was the winner. It could simultaneously...

19. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post

    ...existed. Recently, however, a particular tool has gained widespread popularity – and notoriety – as a tool...

20. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...