We narrowed to 344 results for: GAI

1. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   Blog Post

   ...3-100 per cell, a stabilized promoter buffered against the effects of copy number on reporter gene expression...

2. RANbodies: Reporter Nanobody Fusions

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   Blog Post

   ... et al can be found here. Can’t find a RANbody against your favorite protein? No worries! As more nanobodies...

3. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   Blog Post

   ...anything good. And I will never write anything good again.’ ” For Susan, one of the most satisfying parts ...

4. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ... a kinases in a body part of choice. Zhou et al again used the Raf-MEK-ERK signaling pathway to demonstrate...

5. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...mouse cortex. Image from He Tian. Congrats again to all the recipients! ...

6. Antibodies 101: Polyclonal Antibodies

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   Blog Post

   ...full-length protein that you want to generate antibodies against, or a unique peptide sequence derived from this...

7. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   Blog Post

   ...constantly communicating with a tumor, proving once again that a tumor is always interacting with its surroundings...

8. Celebrating 15 Years of Scientific Sharing

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   Blog Post

   ...here at our new location to grow. We are hiring again and getting ready to embark on new exciting projects...

9. Summer SciComm Series: Cognitive Load

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   Blog Post

   ...you!)  Amount of information and level of detail Again, this is an obvious one on the surface, but it’s...

10. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post

    ...of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the Addgene Blog Learn How...

11. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ...enable you to quickly BLAST your sequencing result against the expected sequence for quick verification, which...

12. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    ...tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells with GFP, at a ...

13. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...from your membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample...

14. Hot Plasmids February 2024

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    Blog Post

    ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

15. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...

16. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent adenovirus...

17. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ... who participate in the course show significant gains in both content knowledge and research skills (8...

18. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...with an antibody. This can artificially select against PPIs that aren't stable outside the native cell...

19. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post

    ...existed. Recently, however, a particular tool has gained widespread popularity – and notoriety – as a tool...

20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    ...transduction; but these methods aren’t stealthy enough to gain access to some cells and tissues, such as human ...