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We narrowed to 1,105 results for: Ide

Showing: 221 - 240 of 1105 results

  1. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...“plasmid” derived as a hybrid of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal...noted that, unlike F-factors, the evidence did not support the idea that R-factors could integrate into... Élie Jacob and François Wollman and became the widely adopted name for these elements. At the time, the...Herbert Boyer, and Charles Brinton concocted a wild idea of using the newly discovered EcoRI enzyme (and ...such as pBR322, pACYC, and pUC were developed to provide higher copy number vectors that could be used in... with our goal of creating a one-stop reference guide for plasmids we've updated and added new content...Datta, Naomi. “Transmissible Drug Resistance in an Epidemic Strain of Salmonella Typhimurium.” The Journal...

  2. Lentiviral Vector Guide

    Type
    Guide
    ... Science Guides Viral Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral...production, in trans refers to genetic elements provided outside of the plasmid containing the gene of interest...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...mutations, or treatments into cells, and has been widely used in the research community for decades. Wild-type...in a host, and exhibit low immunogenicity. This guide contains many viral vector-specific terms and acronyms...gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses ...contains packaging gene env (usually VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G...

  3. Modular Cloning Guide

    Type
    Guide
    ...Molecular Cloning Techniques MoClo Guide Modular Cloning (MoClo) Guide You may also like... Plasmid Kits... restriction enzymes BsaI and BpiI/BbsI to cut outside their recognition site, leaving DNA fragments with...unit (for example, a promoter, a 5' UTR, a signal peptide, a protein-coding gene, and a terminator, as in...plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the ...construction of highly-active Platinum TALENs for use in a wide variety of target organisms. TALEN Kit Genome Engineering...fluorescent proteins, protein tags, and signal peptides, for use in a range of organisms including diatoms...plants and bacteria. References and More Resources Guide to DNA Assembly Techniques by Nicola Patron at The...

  4. Guide to Using Pooled Libraries

    Type
    Guide
    ... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries...amplification protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse... Libraries Jump To: Pooled Library Guide Introduction Library Amplification Types of Screens Resources...powerful tool for forward genetic screening, or identifying previously unknown genes that contribute to a...replicate for that plasmid. Library screens can be divided into two types: positive screens and negative screens...septicum alpha-toxin , and resistance to to the nucleotide analog 6-thioguanine . Next-generation sequencing...Positive Screen In a positive screen, the goal is to identify those cells that survive post-selection. The selective...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... Science Guides Viral Vectors Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral...production, in trans refers to genetic elements provided outside of the plasmid containing the gene of interest...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...mutations, or treatments into cells, and has been widely used in the research community for decades. Wildtype...expression in a host and low immunogenicity. This guide contains many viral vector-specific terms and acronyms...about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral Vectors The genome of gamma-retroviruses...contains packaging gene env ; usually VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G ...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Vectors AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production...watching our Optogenetics 101 video or by visiting Addgene's Optogenetics Guide . Browse optogenetics plasmids...production, in trans refers to genetic elements provided outside of the plasmid containing the gene of interest... vector guide to learn about AAV vector components, production and common uses. Science... Science Guides Viral Vectors...a protein capsid and the viral genetic material inside. The AAV genome is ~4.7 kb long and consists of...viral vectors for gene therapy applications. This guide contains many viral vector-specific terms and acronyms...

  7. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...ssODN: Using Ensembl Transcript ID(s) as an input query, pick crRNA (guide RNA sequence targeting the gene...tagging experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene(s) from Ensembl genome ...coupled to high-throughput sequencing to identify genome-wide binding patterns of the target protein. ... not work in pull-down assays: Epitope tagging provides a solution Proteins - the workhorses of the cell...complexes are then analysed by mass spectrometry to identify the interacting proteins. Similarly, in ChIP-seq.... Both IP/MS and ChIP-seq methods provide a wealth of information about how protein networks work in ...to mention frustrating. One elegant solution to sidestep the antibody problem is to tag the protein of ...

  8. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...bit like a GenBank ID) to all antibodies, animal models, and software tools in the Materials and methods...scope for the journal’s coverage. These guidelines also provide information on a journal’s house style ...Resource Identification Initiative. During this project, authors are asked to add an identifier (a bit ...vivo research and the CARE guidelines for case reports are two sets of guidelines frequently used by editors...information is provided. The clearer you write your paper and the more information you provide on data, materials...publication in a scientific journal requires several considerations. First, it is important to establish the type...audience you want to reach with your paper. Is it the wider scientific community, or are you writing for a specific...

  9. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...Reproducibility Read a blog post on Unique and persistent IDs for improved reproducibility Other resources: Reproducibility...practices could be an actionable way to tackle widespread irreproducibility. Luckily, there were other ...academics and like-minded organizations with similar ideas who were also thinking about reproducibility and...Thanks to Teytelman’s vast network and ability to identify the right opportunities, he was able to bring ...and motivated groups and individuals with similar ideas to actually do something about it. Building a diverse...received, and then when the team started brainstorming ideas for the next workshop, they realized that holding...enable reproducibility practices being adopted by a wider group of researchers. Generating material to empower...

  10. Summer SciComm: Preprints

    Type
    Blog Post
    ...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...used in a preprint provides all the same benefits as sharing those plasmids alongside a journal article...different goals, formats, and processes, and they provide different amounts of access or feedback. One of...in the past few years.   Figure 1: Stand aside, CERN — it's a new age of accelerating science! ...contrast, journal-based peer review is usually a confidential process among a few individuals.  Early-career...especially enjoy the ability to post a preprint as evidence of their productivity that can be shared with ...funding bodies and review committees encourage and consider preprints as demonstration of productivity. Part...

  11. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... a post-doc in the Target ID and Validation Group and says that she decided to do a post-doc at Novartis...library, propagated it, separated it into roughly identical replicate groups, and treated these groups with...cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with the ClonTracer...models. She hopes that the library can be used to identify appropriate, non-overlapping targets for drug ...distinct evolutionary paths to become resistant to epidermal growth factor receptor inhibition." Nature medicine...

  12. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...Looking at the structure of the Cas9 nuclease (PDB ID: 4OO8 and 4UN3), Slaymaker et al. hypothesized that...Benjamin P., et al. "High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects."...As evidenced by all the CRISPR publications, press, and plasmids out there, it’s obvious that CRISPR ...1.1), followed by a technique for detecting genome-wide double strand breaks (BLESS), revealed that these...as predicted, decrease off-target effects genome-wide. Further work by Slaymaker et al. showed that these... al. "Crystal structure of Cas9 in complex with guide RNA and target DNA." Cell 156.5 (2014): 935-949....Website Find CRISPR Plasmids Browse our CRISPR Guide Pages Find Genome Engineering Resources ...

  13. Lab Automation at Addgene

    Type
    Blog Post
    ...removable parts to fit on the deck of the Biomek. The FXP id also able to do high throughput DNA preps and other...purification, and filling vials with agar. I’ll also provide you with some tips on how to automate some simple...protocol and, after looking at a few options, we decided on Beckman Coulter’s Biomek FXP. We had a previous...scale. Using the BIOROBOT Universal Platform is ideal because it eliminates the potential of human error...standard molecular biology techniques Browse our video library Check out our molecular biology reference...

  14. Common Injection Routes in Mice

    Type
    Blog Post
    ...Skill required: beginner Intradermal Intradermal (ID) injections occur between the layers of the skin.... post has provided a good introduction to common injection routes in mice! When considering what method...administering vaccines, pain meds, or other drugs. Identifying the proper injection site is straightforward,...knowledge. Intrathecal injections are used for a wide array of purposes, but mainly for the delivery of...sounds very disruptive, but surprisingly, it is considered minimally invasive. Restraint: anesthesia Location...common. Due to the intubation, this procedure is considered quite invasive. Restraint: anesthesia Location...feeding tube with a bulb on the end, which prevents accidental tears or punctures to the throat. The feeding...

  15. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... It’s possible to associate the depositor’s ORCID ID with the deposit, and a DOI is added to the deposit...integration is described in this video and the Figshare integration in this video.  The ability to link from...making it searchable and shareable, but some ELNs provide better support for reproducibility than others....reproducibility is limited in two ways – only data inside the ELN is reproducible, and only the highly restricted...requirements that emerged from intensive engagement with a wide range of people at the University of Wisconsin in...principles in mind, and in response to further input provided by the research communities at the University ...online file storage tools, as illustrated in this video, and to data stored on institutional and lab servers...

  16. Tips for arabidopsis transformation

    Type
    Blog Post
    ...https://books.google.at/books/about/Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September ...culture into a container that is wide enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic...process right the first time. I’ll lay out some guidelines here. A detailed transformation protocol, as ... et al. (2006) ‘A rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following...

  17. The Challenges of Cell Culture

    Type
    Blog Post
    ...Short Tandem Repeat (STR) profiling can be used to ID the cell line. The same test is so good that it is...many journals strictly require evidence of cell line provenance and identity. The Nature Publishing Group...Resources ATCC Animal Cell Culture Guide Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen...Invitrogen Cell Culture Basics Guide Abcam Cell Culture Guidelines Resources on the Addgene Blog Learn ...culture in the present day Cell culture is a widespread tool used in the fields of oncology, virology...this reason. Unreproducible results are rife. Misidentified or cross-contaminated cells have been known ...Invasion in Renal Cancer Cells, Marker-independent identification of glioma-initiating cells. The International...

  18. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N...differences in guide RNA efficiency. For example, in a two-guide floxed experiment, if one guide cleaves less...from the target exon on either side. They have successfully used guides targeting the same or complementary...on each side. For optimal repair, place the loxP sites precisely at the cut site for each guide. This design...RNP) complexes of Cas9 protein and the separated guide RNA components (crRNA and tracrRNA) for maximum ...given location, you’ll need to design only a single guide and your donor ssDNA. Here, we’ll use the example...homology-directed repair experiments, you’ll want the guide cut site to be as close as possible to your desired...

  19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...Biology. New York: W. H. Freeman, 2000. Bookshelf ID: NBK21668. Matsuno-Yagi, Akemi, and Yasuo Mukohata...neuronal subpopulations, and this represents a major downside in optogenetics (Belzung et al., 2014). This could...genes. Strong cell-specific promoters, on the other side, are too long for rAAVs, which can only package ...Double-floxed Orientation)) is a powerful tool that provides precise temporal and spatial control of gene expression...produce behavioral and electroencephalography evidences of arousal in mice previously subjected to steady-state...., 2019). FLEx vectors represent, therefore, an ideal partner to optogenetics to understand the cellular...

  20. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    .... (2024). A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for ...antibodies when possible. If cost is prohibitive, consider an approach that prioritizes more reproducible... rate. If you need a more permanent knockdown, consider using shRNA vectors instead. With both siRNA and...this approach should also allow for the clear identification of the cell types of interest. By correlating...correlating your data sets, you can increase confidence that the labeling observed represents the expression ... at similar levels in your cell lines, you can consider a cell treatment approach. If there is a cell ...protein. A challenge to this approach is that the identity of the epitope is often not available to confirm...
Showing: 221 - 240 of 1105 results