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We narrowed to 771 results for: LIS

Showing: 221 - 240 of 771 results

  1. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... physical titer are direct p24 measurements via ELISA and qPCR for viral RNA. Measuring p24 levels in ...as there are several commercially available p24 ELISA kits that can quantify p24 levels in a matter of...drawback of this method, however, is that a p24 ELISA measures all p24 in the sample regardless of whether...alternative to direct p24 measurement. Like a p24 ELISA, this method is relatively quick, providing results...than a day, but tends to be less expensive than an ELISA kit. In this approach, viral RNA is first converted...

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire one image, thereby significantly ...  Thank you to our guest bloggers, Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor...Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an Associate Professor at the University....janelia.org/lab/keller-lab http://www.physikalischebiologie.de http://www.lavisionbiotec.com/ultramicroscope-overview.html...

  3. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...contribute openness, clarity, approachability, and professionalism to team meetings and projects. It is crucial...identify your transferable skills? PLoS One recently published an article identifying gaps in scientists' transferrable...communication skills quite a bit. Grab a piece of paper and list all the skills that your current or past job requires... get your mind working, take a look at the short list of transferrable skills here and consider which ...Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning Resources Cross team...

  4. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...path.  A part of the career path story for most established grant professionals is learning to succeed in... with clear budget narratives, logic models, established partnerships and collaborations, and commitments... panels and to review education materials for publishers. When the community orchestra needed a grant ... to enter the grants profession? The four steps listed below can give you the resources and path to help...professional development strategy builds on the steps listed above and formalizes your development. You may ...

  5. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...the fact). #9 Thou shalt make a to-do list, and a to-don’t list Prioritize! Some things can wait and some...This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic...tactics to plan your work and make this happen. Be realistic and: Your co-workers will learn to work within...your family, for yourself and for fun to the to-do list. #10 Thou shalt also have fun I’m really busy, but...

  6. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...sequence assembly of the zebrafish genome was published in 2013, revealing that about 70% of human genes...networks that regulate processes from development to metabolism to fertility. Several zebrafish-specific gene...for generating transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust...zygote transforms into an organism with a clearly established body plan including head, eyes, and tail. Compared...different methods have been developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality...

  7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... encouraged to not just wait for papers to be published, but to engage in ongoing, real-time dissemination...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Lab (@EvoEmbryo) September 24, 2018   Xenopus tropicalis (Gary Gorbsky) This is not a new experimental...with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke) No methods were available to...A paper on stickleback fish science was first published in 1867. It said they were hard to raise because...

  8. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...implementation, and now must focus on managing the established partnership. If you’re willing to do similar ... worked with people outside the department to accomplish a variety of goals. For example, I worked with...from other universities and maintained a mailing list. This project gave me practice reaching out to other...some advice and guidance. The relationships you establish in this way can, of course, lead to jobs and even...common interest so naturally this may be easier to accomplish with people at your organization. You can meet...

  9. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...these people at the time, but I met some very accomplished scientists and some rising stars in the world...world of microbiology, some before they had even established their own labs.  Fried chicken with a side of...worth addressing. Clear expectations have been established for future participants regarding the activities...Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael D....Apologies to anyone I’ve inadvertently left off this list. Many thanks to our guest blogger Joe James from...

  10. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ... category by listing off things that fall into that category? Okay, let’s play. I’ll list the examples...examples and you try to guess the category: ELISA...qPCR...Digital droplet PCR...DNA dot blot...Transduction...can be affected by many experimental factors. To list some complicating factors for quantifying AAV: ...Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant...

  11. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...not enough. A 2013 study showed that out of 238 published scientific articles, only 46% could be reproduced... have software tools that help us efficiently accomplish our daily goals. These tools remind us of our...In their ideal form, ELNs provide users with a holistic means to manage their work, combining data, team...there will be no sharp changes in costs once they establish the system within their institution. In the study...supportive the ELN team is. Once you’ve made your list of ELNs, create some free or demo accounts and schedule...

  12. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    ...open-access journal that enables high school students to publish peer-reviewed scientific research. You’ll meet ...submitted to the journal with funding from Addgene. Listen to the interview with the Journal of Emerging Investigators...Arooba Ahmed, Jiachen Lee, and Jillian Parker who published their work on a protein required for cytokinesis... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  13. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

    Type
    Blog Post
    ...Plasmids Podcast Listen to our interview with synthetic biology researcher Tom Ellis Resources at Addgene.org... in George Church’s lab as a graduate student. Listen to the interview with Wei Leong Chew! Podcast breakdown... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  14. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides 72...first pay attention to the validated applications listed on the vendor’s website and choose an antibody ...applications, pay close attention to the controls listed on the vendor’s website. Typically, the vendor ...method such as an enzyme-linked immunosorbent assay (ELISA) (Aydin et al., 2015). Post-translational modifications...

  15. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut...nevertheless still lead to activity has been established, and can be used to identify sites that are likely... for functional genomics, with an ever-expanding list of tools to probe gene function. The best tools ....1093/nar/gkt555 Resources at the Addgene Blog Listen to our Podcast with John Doench Learn How to Conduct...

  16. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...equipment that will help accelerate his research. Listen to learn more about Dr. Kwapata’s current work ...more scientific collaboration across the globe. Listen here! Podcast Breakdown (Time: Topic) 0:00-1:00... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...through Seeding Labs Resources on the Addgene Blog Listen to Additional Addgene Podcasts Learn How You Can...

  17. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...now works on protein therapeutics at Novartis - listen to learn more about Crystal’s exciting career path...new projects when old ones prove unsuccessful. Listen to the interview with Crystal Shih Podcast breakdown...applying for industry jobs and deciphering job listings. Mobility between different positions at Novartis... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  18. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ... own nonprofit, this is the episode for you.   Listen to the interview with Addgene's founders Podcast...Developing Addgene as a modern e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene...Harvard University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  19. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...who creates human-animal hybrids. This book is published 30 years after the seminal work of Gregor Mendel...into the twenty amino acids. Once scientists established that DNA encoded all the information necessary...giants such as Isaac Asimov, Arthur C. Clarke, James Blish, and John W. Campbell. This period is termed as ...specific.  Jurassic Park by Michael Crichton was published in 1990, just a year after the discovery of meganucleases...Packer MS, Badran AH, Bryson DI, Liu DR (2018) Publisher Correction: Programmable base editing of A•T to...

  20. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...CRISPR use grows across the life sciences and to establish standardized validation techniques for academia...starting point for most researchers as it provides a list of putative off-target sites that they can later...limited to off-target sites of <4 bp. The off-target list is also generally weighted by the position of the...silico prediction to create a more comprehensive list of off-target editing events. Two of the most common.... Outside of the lab, he works as a freelance journalist and science writer with a focus on the ways science...
Showing: 221 - 240 of 771 results