Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 526 results for: MEA;

Showing: 221 - 240 of 526 results

  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...frameshift or 21+ bp indel. This score is a useful measure to determine how many of the contributing indels...frameshift or 21+ bp indel. This score is a useful measure for those who are interested in understanding how...

  2. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... a result, mesothelioma is often misdiagnosed, meaning that by the time it has caught it has usually started...in the body. Such misdiagnosis and late discovery mean that most mesothelioma patients have an extremely...

  3. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...Share science, video usurps the written word as the means for communicating research. Short videos of 3 to... helps researchers go beyond citation rates to measure the impact of their contribution. It offers a 5...

  5. The Challenges of Cell Culture

    Type
    Blog Post
    ...experiments carried out on low passage cells and this means you can regularly discard the cells you are working... actual flasks of cells in the incubator). This means any other scientist who wishes to use these cells...

  6. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...plant sciences “transient transformation” usual means that neither a stable cell line or plant is regenerated...GUS and are therefore good temporal reporters to measure promoter activity, for example. Useful positive...

  7. Lab to Office Culture Shock

    Type
    Blog Post
    ...think “scientist.” But leaving the bench does not mean you’re not a scientist anymore. Being a scientist...their entire career. Yet even success in academia means you’ll eventually end up as a PI working what is...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...it’s difficult to get data out of the ELN. This means that reproducibility is limited in two ways – only...external file storage resources remain live. This means that someone who wants to query and attempt to reproduce...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    ... natural selection of sequences that evolve a measurable property of a protein. As a proof of concept,... allow for sensitive and high spatiotemporal measurements of norepinephrine. These norepinephrine sensors...

  10. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...complexes, wash away unbound protein, elute and measure the target. However, the two different approaches...the additional benefit of permanent linkage. This means that the primary antibody will not co-elute with...

  11. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...transient transfection, the vector remains episomal, meaning it stays separate from the genome. One option for...replicate during the course of your experiment, meaning you don’t have to worry about vector replication...

  12. How to Be an Excellent Trainee

    Type
    Blog Post
    ...you remember everything? Write it all down! And I mean everything! The bare minimum should be taking notes...invaluable. With a little thought you can build meaningful relationships in your new lab, learn the required...

  14. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...recognize and bind to specific promoter elements. This means that the promoter present in your plasmid backbone...bacterial and mammalian promoters. These lists is by no means exhaustive, but should be a good place to start ...

  15. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...proteins can be accurately determined in vitro by measuring the fluorescence intensity of purified protein..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3...

  16. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ... be aware of deadlines and think about what they mean in terms of the utility of your tasks. In my cell...accomplish these goals. Practically speaking, this means I write everything in a Word or google doc that ...

  17. Evolution of Lab Techniques

    Type
    Blog Post
    ...DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the different...nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move from sequencing ...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...rapidly dispelled. She would ask me, "Is this what you mean? Are you trying to say this?" I'd say, "No, I'm ...a lab, getting grants, and writing papers? This means that once you get an interview, your previous success...

  20. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...secondary antibodies are generally polyclonal, which means that multiple secondary molecules can bind to a ...target and sample type. Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking...
Showing: 221 - 240 of 526 results