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...replication (which controls the host range and copy number of the plasmid) and typically include a gene that...

...scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist...

...present in large numbers, 50 or more per cell, and have a high copy number. High copy number plasmids should...copy and low copy plasmids? The copy number refers to the number of copies of an individual plasmid within... of bacteria and is used to grow up sufficient numbers of bacteria necessary to isolate enough plasmid...Figure 2). Large plasmids usually have a low copy number (approximately one or two copies per cell) and ...

...with broccoli aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing...

...Editing, gets over 4,000 views a month. To me, these numbers show that this basic concept of gRNA design is ...

...biotin incorporation and to a lesser extent, the number of added biotins per molecule cannot be well-controlled...

...Addgene Cas9 and NgAgo head to head NgAgo has a number of unique features compared to Cas9. These include...

...transcripts of interest without an increase in the number of steps in the protocol. IgnaviCas9’s incredible...

... for studying autophagy focused on counting the number of autophagosomes. This approach, however, has ...

...an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for a variety...

... response, I was still able to get through to a number of experts, including Harvard/MIT faculty, venture...

...upon the type of transcriptional regulator or the number of designed scaffold binding sites. With hundreds...

...what options lie ahead of me? Like a considerable number of researchers, I had high hopes of securing tenure...

...you are in a lab, your PI is likely working on a number of them. Find one to apply to or offer to help ...

... with a high MOI, which is beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR...

...an audience?  An audience can be defined by any number of characteristics. Common ones include age range...

...to dive into how they’re used! We have a growing number of Viral Vectors 101 posts about each type, so ...

...Applications of epitope tags   The tags A number of epitope tags exist – all with individual optimizations...

...your vector is large (>10 kb) or has a low copy number, try following protocols for low copy plasmids....

...neutralization. “That degree of escape from a very small number of mutations was surprising,” says Balazs.   ...