We narrowed to 420 results for: Numb

1. Some Like it Hot: Thermostable GeoCas9

   Type

   Blog Post

   ...Cas9 is the genome editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila...

2. Sleeping Beauty Awakens for Genome Engineering

   Type

   Blog Post

   ...then by increasing the consensus between a greater number of extinct transposon sequences and then testing...

3. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

   Type

   Blog Post

   ...visitors to the poster were impressed to see how the numbers of plasmid genome engineering tools – both deposited...

4. Choosing a Good Mentor for Scientists

   Type

   Blog Post

   ...scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist...

5. Protocol - How to Inoculate a Bacterial Culture

   Type

   Protocol

   ...present in large numbers, 50 or more per cell and have a high copy number. High copy number plasmids should...copy and low copy plasmids? The copy number refers to the number of copies of an individual plasmid within... of bacteria and is used to grow up sufficient numbers of bacteria necessary to isolate enough plasmid...bacterial cell. Large plasmids usually have a low copy number (approximately one or two copies per cell) and ...

6. CRISPR Challenges: Standardization and Homology Directed Repair

   Type

   Blog Post

   ...Editing, gets over 4,000 views a month. To me, these numbers show that this basic concept of gRNA design is ...

7. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   ...biotin incorporation and to a lesser extent, the number of added biotins per molecule cannot be well-controlled...

8. Intro to Our Summer SciComm Series!

   Type

   Blog Post

   ...an audience?  An audience can be defined by any number of characteristics. Common ones include age range...

9. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

   Type

   Blog Post

   ...your vector is large (>10 kb) or has a low copy number, try following protocols for low copy plasmids....

10. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type

    Blog Post

    ...you are in a lab, your PI is likely working on a number of them. Find one to apply to or offer to help ...

11. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type

    Blog Post

    ...to dive into how they’re used! We have a growing number of Viral Vectors 101 posts about each type, so ...

12. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type

    Blog Post

    ...transcripts of interest without an increase in the number of steps in the protocol. IgnaviCas9’s incredible...

13. Viral Vectors 101: Transductions

    Type

    Blog Post

    ...an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for a variety...

14. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    ... for studying autophagy focused on counting the number of autophagosomes. This approach, however, has ...

15. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type

    Blog Post

    ...with broccoli aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing...

16. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    ... with a high MOI, which is beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR...

17. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type

    Blog Post

    ...helpful to report the genetic background and catalog number or RRID if it exists. This is helpful because viewers...

18. Antibodies 101: Epitope Tags

    Type

    Blog Post

    ...Applications of epitope tags   The tags A number of epitope tags exist – all with individual optimizations...

19. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    ...neutralization. “That degree of escape from a very small number of mutations was surprising,” says Balazs.   ...

20. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    ...few years for a new project to reach a sufficient number of distributions to cover costs, which can limit...