...techniques, such as Oxford Nanopore sequencing or PacBio SMRT sequencing, (which differ from the short-read...1-25 kb), such as Oxford Nanopore sequencing or PacBio SMRT sequencing. These longer reads are able to... Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production. Biotechnol...

... professional capacity generated during this process result directly in positive impacts on the lives ... a level of sympathy that is difficult to find. Impact of the International Mentorship Program and the...

...between the transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...

... marker to identify and enrich positive cells Packaging and envelope plasmids provide the necessary components...particles (for details, see our AAV Guide ) ∼4.5 kb packaging limit (only compatible with smaller Cas enzymes...

... a bit like a Fedex delivery. Your fluorophore package needs to go to the proper mailbox (i.e., a binding...that can nonspecifically soak up your fluorophore package. One important concept in FRET experimental design...synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian ...

... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition to using a new UNG...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...

...- Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial ...SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA ...

...–6 67,366 (Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E....E. coli Zhang N/A N/A N/A - The protospacers contained in the library represent all 4096 (4 6 ) combinations...

...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology...

... year to give our lab and shipping teams enough space at HQ and for some of us, that means spending more...co-workers” have made their way onto our Slack workspace over the last year. Now, we’ve collected photos...

...Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Thorben Dammeyer 54520 ...transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of the proteins that enter the...

...week to celebrate the scientists who make a big impact in open science. Depositors consistently contribute...depositor fun facts for a quick way to grasp the big impacts depositors have on science globally. *All data ...

...titering. Our newest viral vector service, AAV Packaged on Request  has seen a fantastic response from...our in-stock viral vectors or consider our AAV Packaged on Request service, which offers over 3,000 eligible...

...to younger graduate students   Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...studies developmental gene regulation with Dr. Angela DePace in the Department of Systems Biology, and he tweets...

...and moved to our packing area.Using the barcode system, the correct plasmids are packed into their boxes...

...Running your transfer in the cold room or packing the box with ice packs can help keep the system cool, as can...overheating. Try running it in a cold room or putting ice packs in and/or around the gel box. If overheating remains...

...the talent pipeline sets the world up for reduced impact of scientists and science. The pandemic has demonstrated...of priorities for pd|hub which include building capacity to identify the most effective practices and disseminating...

...Sequence Table. Disclaimer: The efficacy of gRNAs is impacted by the target location, target sequence, and model...multiple keywords that are separated by a single space to get even more specific. For example, to show ...

...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the...

...role in fighting the pandemic could be even more impactful by reaching more scientists. Many members of the...past months, we’re excited to see our open sharing impact grow beyond COVID-19. But, we can’t do this alone...