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We narrowed to 564 results for: PAC;

Showing: 221 - 240 of 564 results
  1. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...routinely use Nanopore technology to sequence our packaged viral DNA for quality control. This gives us a...the issue and resolve the assembly. Keeping up the pace Our QC team constantly faces the challenge of keeping... backend, has enabled the QC to keep up with the pace of deposits while maintaining quality. Maintaining...
  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...resources: ImpactStory, a website that helps researchers go beyond citation rates to measure the impact of their...Scientists Read about the Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in ...
  3. TALEN Plasmids and Kits

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    Collection
    ...Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory pack contains modified pFUS array vectors...
  4. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post
    ...Bear in mind, the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent ...” gate in the beginning should not have a great impact on your analysis, and I often choose a looser gate...separation pattern largely stays the same and does not impact my ability to draw a proper gate, I am free to ...
  5. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post
    ...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...
  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...in mind, but likely will not have a significant impact on your data. You are trying to generate a knockout...depending on your platform, off-target effects should impact your experimental design differently. Yes, it is...optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few...
  7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ... and infect specific cells. This protein shell packages the vector’s genetic material, and variations ...serotypes at Addgene. Serotype In-stock viral prep Packaged on Request AAV1  ✓ ✓ AAV2 ✓ ✓ AAV2-retro...Resources on Addgene.org AAV Guide AAV Data Hub AAV Packaged On Request Browse all in-stock AAV preps Browse...
  8. Plasmids 101: Dimers and Multimers

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    Blog Post
    ...techniques, such as Oxford Nanopore sequencing or PacBio SMRT sequencing, (which differ from the short-read...1-25 kb), such as Oxford Nanopore sequencing or PacBio SMRT sequencing. These longer reads are able to... Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production. Biotechnol...
  9. Neurodegeneration Research Collection

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    Collection
    ... to using plasmid pooled libraries . Use the ultracompact and programmable endonuclease, TnpBmax , for...Community (Link opens in a new window) Research workspace on Protocols.io (Link opens in a new window) ....
  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...between the transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...
  11. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... a bit like a Fedex delivery. Your fluorophore package needs to go to the proper mailbox (i.e., a binding...that can nonspecifically soak up your fluorophore package. One important concept in FRET experimental design...synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian ...
  12. CRISPR 101: Cytosine Transversion Editors

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    Blog Post
    ... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition to using a new UNG...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...
  13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...Addgene has an extensive collection of plasmids for packaging and expression, see our dedicated Adeno-associated...Addgene has an extensive collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids...
  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...- Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial ...SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA ...
  15. Plasmids for Stem Cell Research

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    Collection
    ...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology...
  16. Tetracycline Inducible Expression

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    Collection
    ...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator...H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional activators...
  17. Participate in the Pets of Addgene Scavenger Hunt

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    Blog Post
    ... year to give our lab and shipping teams enough space at HQ and for some of us, that means spending more...co-workers” have made their way onto our Slack workspace over the last year. Now, we’ve collected photos...
  18. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...week to celebrate the scientists who make a big impact in open science. Depositors consistently contribute...depositor fun facts for a quick way to grasp the big impacts depositors have on science globally. *All data ...
  19. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...titering. Our newest viral vector service, AAV Packaged on Request  has seen a fantastic response from...our in-stock viral vectors or consider our AAV Packaged on Request service, which offers over 3,000 eligible...
Showing: 221 - 240 of 564 results