We narrowed to 638 results for: PAN

...probed for their suitability in iPSC programming spanning from fibroblasts and keratinocytes to peripheral... allows access to a larger number of willing participants. While fibroblasts obtained from aged patients...success [1]. Fibroblasts need to be cultured and expanded in vitro for several passages to produce an adequate...

...Young. The use of CRISPR/Cas9 for gene editing has expanded since its adaptation for use in mammalian cells...ordering the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...order them yourself through whatever DNA supply company you currently use. You can also find many previously...

...recombinase variants to optimize PASSIGE efficiency (Pandey et al., 2024). The best-performing variant yielded...sequence between two attR/L sites. Image reused from Pandey et al. 2024 under CC-BY 4.0 license.   An, ...doi: https://doi.org/10.1038/s41587-021-01133-w Pandey, S., Gao, X. D., Krasnow, N. A., McElroy, A., Tao...

....  How do you find validated antibodies? Many companies that produce and distribute antibodies validate...through their publications or as a review on a company website. It is important to review the data and...can help you compare antibodies from different companies, such as Validated Antibody Database and CiteAb...

...modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation in an array...PubMed PMID: 15790858. 5. Park, Hee-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine... Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013):...

...labeling compared to immunoprecipitation. The top panel depicts the identification of heat-shock proteins...in HCT116 cells (colorectal cancer). The bottom panel depicts the identification of general heat-shock...different interneuron populations. The enhancers span all four major classes of GABAergic interneurons...

...cle10ts) allele), increasing experimental options. Expanding and improving the CRISPR/Cas editing toolbox for...need to segregate away co-conversion markers. Expanding beyond Cas9 Since the development of CRISPR/Cas9...https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B (2015) Streamlined...

...by the trial. During clinical trials, expansion of participants based on early results can enhance the...treatment regimens were not chosen carefully. Participants in clinical trials should be picked based on...

...allowing users to easily isolate and subsequently expand monoclonal cultures.  Interpreting flow cytometry...experimental sample. In addition, you should include a panel of “fluorescence-minus one” controls, samples stained...experiment except one. The fluorescence-minus one panel will help you set up gating during data analysis...

... of more stable, brighter, and specific dyes, expanding the capabilities of fluorescence-based techniques...® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling capabilities of this platform...Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox via new far-red-emitting...

...distributors in China, Korea, Japan, India, and Brazil. Through an expansion of our export license application...different countries, into our repository, while expanding global access to our repository through our distributor...partners, with the launch of antibodies in China and expansion of viral services available in South Korea.  ...educational resources, with the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming... Viral Vectors 101 eBook. We’ll also focus on expanding international access to the Addgene repository... efficiencies typically adopted by for-profit companies, while making use of our nonprofit status to achieve...

...process ensures that any potential sequence discrepancies can be caught prior to distribution. Catching...issue? At Addgene, a QC issue is defined as a discrepancy that we believe may affect the function of the...decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited to Addgene...resolve. Sequencing and assembly issues Sequence discrepancies are not always a result of mutations in the ...will contact the depositing lab to review the discrepancies. Often, the identified issues will not affect...requesting scientists aware of the identified discrepancy in the plasmid, and that it is not known to impact...plasmids and provide transparency in sequence discrepancies. While Addgene’s QC process adds time to the...

... large sequences (up to 10 kb) into the genome (Pandey et al., 2024; Yarnall et al., 2023) Prime editing...doi.org/10.1038/s41587-021-01025-z Doman, J. L., Pandey, S., Neugebauer, M. E., An, M., Davis, J. R., Randolph...410. https://doi.org/10.1038/s41587-021-01039-7 Pandey, S., Gao, X. D., Krasnow, N. A., McElroy, A., Tao...

...within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should also ...cell tracker dyes are helpful in designing such panels, so that pre-stained cell lines can be mixed together...particularly useful when designing larger multicolour panels, where different manufacturers may have the same...

...made improvements to base editors, including: expanding targeting scope improving editing efficiency decreasing...new base editors with distinct PAM sequences, expanding the number of available target sites for base ... deaminases. Recent efforts have also yielded a panel of Tad-CBEs that can precisely target single cytosines...development of near-PAMless Cas9 variants significantly expands the library of accessible genomic targets for base...variants, BE3 editing window variants   Greatly expands the number of target loci for base editing Rees... A., Arbab, M., Krasnow, N. A., McElroy, A. N., Pandey, S., Doman, J. L., Huang, T. P., Raguram, A., Banskota...2019). Continuous evolution of base editors with expanded target compatibility and improved activity. Nature...

...Genome Engineering Using CRISPR/Cas Systems has expanded out of control. To further consolidate these FAQs...the donor. For example, choosing target sites that span the knock-in gene. For making mutations, one good...Laganière, J., Cheng, A. W., Hockemeyer, D., Gao, Q., Alagappan, R., Khurana, V., Golbe, L. I., Myers, R. H., ...

...cancers, including a high percentage (up to 95%) of pancreatic cancers. Ras oncogenes have been difficult to...sequence homology (usually 30-40bp overlaps). To expand the capabilities of Gibson assembly and make a ...scientists use GMAP, there will be a continuous expansion of compatible backbones, promoters, and genes ...depositing your new plasmids with Addgene to help expand the collection! The Jacks lab has made several ...

...the globe. As the pandemic slowed, Addgene began to think about how we could expand our support for the...Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve as access...celebrating Addgene's 10 year anniversary.   Expanding our work In March 2015, Addgene reached the 500,000...over 114,600 viral preps. And in 2025, we’ll be expanding our viral vectors service to include AAV Packaged... the world changed in response to the COVID-19 pandemic, and Addgene pivoted to support researchers combatting...

...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...

...applying to the specific company or lab. Take some time to research the lab or company via their papers and...ddPCR experience. In your cover letter, you can expand on that by describing how you developed and used...place to state why you’re applying to the job, company, and/or lab, and how you feel connected to their...apply for [title of the position] at [name of the company/organization]. I am a [adjective that describes... are interested in working for this lab and/or company. Remember to proofread your cover letter, and ...