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We narrowed to 653 results for: PPL

Showing: 221 - 240 of 653 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...transducing primary cells. What if, instead of supplying a DNA construct, they could simply induce cells...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...

  2. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...on the marketing or business background of the applicant. However, although this position did look for ...experience or a marketing interest that would be able to apply themselves immediately to the position. In case ...understand enough to relate it to the position I was applying for. After all I was only going to be interviewed...

  3. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...packaged into viral vectors for potential in vivo applications or multiplex CRISPR screening.   Using tRNAs...and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided...construct DAP array for multiplex gene editing applications, we provide detailed manual and online design...

  4. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental...Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology,...

  5. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...iontophoresis (applying small electric currents via inserted electrodes) may be applied, but these methods...intracellularly spread away from the site of application and aid in visualization of the morphology of...into muscle) as opposed to direct intracerebral application, which is more invasive and has a much higher...

  6. Hot Plasmids - May 2022

    Type
    Blog Post
    ...optimized with zebrafish in mind, it can easily be applied to other systems.    Find BLITZ Plasmids here! ...Targeted Evolution method (M-CREATE), in which one applies both positive and negative selective pressures ...clonal multiplexing of cells in live imaging application. Each visual barcode consists of a fluorescent...

  7. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression...Fishell   Optogenetics Optogenetics continues to supply neuroscience with ever more efficient tools for...

  8. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ... production, they can be helpful for certain applications and aid in ease of use. Our lentiviral guide...adenovirus genes – E4, E2a, and VA – which are supplied on a helper plasmid and mediate efficient viral...making it replication deficient. This is typically supplied by the production cell line (HEK-293) during production...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this... this blog we will focus on those which can be applied in cell culture and animal models primarily. Fun...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct...

  10. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...interview or whether your receive the next grant you apply for. After all, the only value your work will have... slide, think about whether the same principle applies to other slides in your deck.  If someone asks ...stay tuned to our website and social channels to apply for our future events. In addition to this annual...

  11. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...favorite FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if...got a useful tool, there’s likely someway you can apply it in neuroscience. AAV tools from Addgene are already...

  12. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...with the same genetic background and do an apples-to-apples comparison of all those mutations. If you ...different genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare...

  13. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...research questions. CombiGEM has been successfully applied to functionally characterize combinatorial gene...realization of a variety of perturbations and applications in functional genomics, cell reprogramming, ...He is particularly interested in developing and applying new technologies to interrogate and understand...

  14. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ... Tang and Cepko were eager to see if they could apply GFP regulation to other types of proteins. They ...systems are easily adaptable to neuroscience applications, including optogenetics, and they should make...Tang note that their scaffold system is likely applicable to many types of proteins. For proteins with ...

  15. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ... reduced off-target editing frequencies. For applications that require high DNA editing specificity, for...deamination, an important attribute for therapeutic application. ABE8s demonstrate an overall improved base editing...editors with increased activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038...

  16. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods...to increased intracellular calcium. It has been applied to mark active populations of neurons in vivo to...Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction to AAV ...

  17. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate ... of chromatin interaction networks  A second application for CAPTURE 2.0 is the construction of spatial...temporal changes in chromatin interactions A third application for CAPTURE 2.0 is detection of temporal changes...

  18. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...accuracy of assembly. The fidelity data can be applied to derive similar high-fidelity overhangs for any...Kucera is a Principal Development Scientist of Applications & Product Development focusing on DNA cloning...Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias...

  19. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... experimental, biotechnological, and medical applications. Researches can visualize proteins in vivo by...protein you need   Your plans for downstream applications In this blog post we will summarize some of...acids that are useful in different downstream applications. Cell-free expression systems however, are expensive...

  20. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...
Showing: 221 - 240 of 653 results