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We narrowed to 399 results for: PREP

Showing: 221 - 240 of 399 results

  1. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus...without investing time and labour on reagent preparation in the lab. Figure 3: csRNP-assisted epitope...temperature for 10 min. Deliver csRNP into cells: Prepare cells for transfection, add csRNP and ssODN to ...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Packaging Services Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids ...more information. Browse Addgene’s in-stock viral preps . AAV retrograde (AAVrg) Directed evolution has ...were discovered as a contaminant of adenovirus preparations, which is how they got their name, but they ...

  3. Our New Antibody Mascot is....

    Type
    Blog Post
    ...our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent distribution...quest. Keep an eye out for Abi as they help us prepare for our upcoming antibody launch in late March!...

  4. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...gene expression! We've also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool...Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool...

  5. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...that too few of these trainees are sufficiently prepared during their 6-12+ (!) years of training to get... finding ways to provide new types of career preparedness training for their scientists. I have worked...

  8. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...conduct a two hour long interview. I anxiously prepared for the call. The length of this interview was...was to do for my time at Addgene. This training prepared me for the independent work on the QC team. I ...

  9. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...for a successful application. It takes time to prepare an excellent resume…it is worth it. Read here to... to get help if you are having trouble preparing your most excellent resume. 2) Follow job boards and ...

  10. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... when compared to the conventional AAVs (AAV preparations were always normalized to their genomic titers...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  14. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...brought into the hood. A 48-well plate is also prepared with about 200 ul of culture medium containing...of single clones obtained (on occasion we have prepared two 48-well plates of clones) which enables investigators...

  15. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...a three color panel or a 40 color panel! When preparing a spectral flow experiment and panel, there are...first run a new panel on a spectral cytometer, you prepare reference controls of both beads and cells for ...

  16. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...group dependencies  Take, for instance, a recent preprint released by the Boehm and Beroukhim labs at the...ones made available through the work done in this preprint.   Resources and references References Germline...

  17. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...package. There are three steps to chalk talk preparation: Define your research vision. Organize your ...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach...

  18. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...space where you can address the issue head on. Prepare for this time as if it were a meeting. Gather the...change with them. If you can, set aside time to prepare for future challenges. This doesn’t have to be ...

  19. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ... are institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st... the 2015 and 2016 Future of Research Symposia Prepare Yourself for a New Career in Science with Our Career...

  20. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...Below is a step-by-step overview of the protocol: Prepare ssDNA template: The wild-type plasmid backbone ...means that if the BbvCI enzyme used for ssDNA preparation nicks the anti-sense strand, then the ssDNA template...
Showing: 221 - 240 of 399 results