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  1. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... when compared to the conventional AAVs (AAV preparations were always normalized to their genomic titers...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  5. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...a three color panel or a 40 color panel! When preparing a spectral flow experiment and panel, there are...first run a new panel on a spectral cytometer, you prepare reference controls of both beads and cells for ...

  6. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...brought into the hood. A 48-well plate is also prepared with about 200 ul of culture medium containing...of single clones obtained (on occasion we have prepared two 48-well plates of clones) which enables investigators...

  7. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...group dependencies  Take, for instance, a recent preprint released by the Boehm and Beroukhim labs at the...ones made available through the work done in this preprint.   Resources and references References Germline...

  8. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...package. There are three steps to chalk talk preparation: Define your research vision. Organize your ...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach...

  9. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...space where you can address the issue head on. Prepare for this time as if it were a meeting. Gather the...change with them. If you can, set aside time to prepare for future challenges. This doesn’t have to be ...

  10. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ... are institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st... the 2015 and 2016 Future of Research Symposia Prepare Yourself for a New Career in Science with Our Career...

  11. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...finer points of your techniques. You DO need to be prepared to know the literature of your field and all of...absolute minimum, can appreciate their research. Be prepared to discuss other people's research. Nothing turns...

  12. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...Below is a step-by-step overview of the protocol: Prepare ssDNA template: The wild-type plasmid backbone ...means that if the BbvCI enzyme used for ssDNA preparation nicks the anti-sense strand, then the ssDNA template...

  13. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...depend on the target and sample type. Sample Preparation This step can involve multiple smaller steps...Preservation and Imaging Similar to Sample Preparation, this step can involve multiple smaller steps...

  14. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... the academic research environment is setup to prepare trainees for further work in academia. In fact ...Increase the number of science masters programs to prepare scientists for positions outside of academia Require...

  15. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...really like? Gather data of course. Ask questions. Prepare by going sleuthing on the lab website, publication...ensure that you are expanding your options and preparing for both academic and non-academic career paths...

  16. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...successfully identified using this method. Step 1: Prepare a random fragment library of phage DNA This first...purification, but overnight at 30°C is a good start. Prepare a lysis solution containing your favourite bacterial...

  17. Finding Your Science Policy Path

    Type
    Blog Post
    ...is science communication so an outsized way to prepare is to do lots of writing and presenting for non-scientist... learn more! Figure 2: The many ways to prepare for a science policy career. Image used with permission...

  18. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...corresponding author publications (published, not in preparation) A history of successful funding (fellowship ...perform, and interpret experiments. You'll be better prepared for your future intended career and you'll become...

  19. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...depends on a variety of factors including sample preparation, staining procedures, and controls. To prevent...multicolor flow cytometry experiment, users must also prepare sets of controls to aid with compensation. Compensation...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...increase the activity of neurons from brain slice preparations. Magneto2.0 was also tested in vivo by expression... Seeliger MA, Levinson NM, Chodera JD. bioRxiv preprint. 2016. Full Text. Seeliger MA, Young M, Henderso...
Showing: 221 - 240 of 388 results