We narrowed to 749 results for: REV

... such challenges involves using knowledge from previous consulting experiences, educational training, ...productivity, and many have mechanisms in place to prevent the burden from getting overwhelming. These mechanisms...teams are facing a crunch, and using lessons from previous cases to better staff future teams. As with any...how much my scientific background, training in reviewing literature, and critical analyses skills were ...

...In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli...over-expressing a foreign protein in E. coli. We will review the potential pitfalls of recombinant protein expression... problem for toxic protein products, which can prevent the culture from reaching the desired density within...

...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get...primer and the downstream restriction site to the reverse primer. Next, we need to examine the DNA sequence...homology to the ORF). We now need to generate the reverse-complement of this sequence so that we can successfully...successfully amplify the ORF. You can generate the reverse-complement using existing software (a quick internet...others). If we put the sequence we chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA...digestion, you will need to use a phosphatase to prevent re-circularization of the vector. You should treat...

...strand , meaning that the suffix will need to be reverse complimented for primer design. The restriction...

...environment. We’ve covered five model organisms in a previous blog post, but here are five more model organisms...conserved across species, studying the axolotl may reveal the potential humans have for a similar degree ... the sequencing of the entire axolotl genome, revealing an abundance of genes related to limb regeneration...Model for Regeneration and Aging Research: A Mini-Review. Gerontology, 57(6), 565–571. https://doi.org/10.1159...

... But conflict can also be good. In science, a reviewer’s critical comments on your manuscript is a form.... As you perform the experiments to address a reviewer’s concerns, you are making your hypothesis stronger...majority of conflicts I address in my current role revolve around missed tasks, stalled projects, and disagreements...particular teams at that given time. I think about how previous iterations of those teams resolved similar issues...

...their publications or as a review on a company website. It is important to review the data and protocols ...Uhlen, et al. 2016 is a good place to start.) Reviewing available antibodies can seem overwhelming, especially...available resources and give yourself time to carefully review your options. If possible, try to select a couple...

...have higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1)....protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For detection of...have lower background and higher contrast than previously developed NIR split probes (Tchekanda et al.,...near-infrared calcium indicators in this recent review (Shcherbakova et al., 2021).   Table 1: Properties...

...unlikely to be found in your gene of interest) and prevent incorporation of extensive sequences used for cloning...Translation of the standardized leader peptide prevents secondary structures in the mRNA encoding your...genes from a single transcript, or if you want to prevent read-thru transcription from resulting in the production...Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance." Antimicrobial...

... the HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to...the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al. designed...HIV-1 cleavage allows T-cells to survive, but preventing re-infection would require sustained expression...infects over 250 million people worldwide. As we’ve previously seen in the CRISPR field, the lessons learned...

...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References George...

...protein. Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem.67.1.509  More...Protein (GFP) and other fluorescent proteins revolutionized live-cell protein detection, their bulk (~27...which lead to cystic fibrosis, one of the most prevalent inherited diseases. The authors investigated the...

...rAAVs, can sound almost too good to be true! In a previous post, we covered systemic capsids, which allow...1, rAAV2/2, and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish...(also known as AAVrg) We’ve covered AAVrg in a previous blog post, but to summarize briefly: AAVrg is ...variables, so be sure to check the literature for previous work in your experimental system. Table 3: AAV...

... activation and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive...the LOV domain blocks the enzyme binding domain preventing downstream signaling pathways. Light exposure...optimize the intensities of light sources used to prevent activation of both tools with each light source....  Finding the right information When using a previously published system in your own work, you’ll need...

...modification was impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger...silent, they could identify the target genes of previously uncharacterized enhancers. The popular TALEN-...CRISPR activators and repressors are thought to be reversible once the effector is inactivated/removed from...KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced methylation...

...sugar-containing molecules in molecular biology labs (for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology... have visible colonies growing on the membrane wherever an E. coli colony expressing a functional binding...  References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications for old resources...Bases in Bacteria and Their Viruses." Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David...

...everything there is to know is a strange and exciting revelation. Science in any field can generally be applied...or not. Choose wisely This point ties into the previous one too. I would recommend applying for multiple...factors that can make or break a lab experience. Previous Addgene blogs have discussed the process that ... but I’ve learned the value in finding mentors wherever I go. This story by Dr. Erika Moore in Science...

...account or by email to [email protected] . After we review and approve the MTA documents, your order will ...

...yeast, flies, and even plants! In this blog we will review common uses of MoClo and the kits Addgene offers...the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015). This kit...2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression, ...Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American...

... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...suitable for targeting FRET acceptors to proteins (reviewed in [Yan & Bruchez, 2015]). To get you started,...HTRF: a technology tailored for drug discovery–a review of theoretical aspects and recent applications....energy transfer as a spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID...