Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 569 results for: SHA

Showing: 221 - 240 of 569 results

  1. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Resource Center for Rett Syndrome research. With a shared passion for facilitating research and discovery...Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies, founded on Gray’s work, are each...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...

  2. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo genome...KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL,...

  3. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...That is, until earlier members of our lab - the Shapiro Lab - developed Acoustic Reporter Genes (ARGs),...studying bioengineering. They are both members of the Shapiro lab at Caltech.   References and Resources References...., Ho, G. H., Sawyer, D. P., Bourdeau, R. W. & Shapiro, M. G. Ultrasound imaging of gene expression in...

  4. Bioinformatics at Addgene

    Type
    Blog Post
    ...of Nextflow supporters existed who had created a shared resource of pipelines for genomic data. Thanks ...safely operates in isolation, and can be easily shared with others without any complicated setup. We created...needed a user interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment...

  5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...when bacteria of different evolutionary lineages share genes that can improve chances of survival for an...experiences of the mentors were very valuable when shared with the next generation of scientists. The connection...Tiffany Lowe-Power, Eric Griffis, Janet Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa...

  6. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...solution for which they are sure that there will be no sharp changes in costs once they establish the system ...functionalities include comments, notifications, sharing files, assigning team members to certain tasks,...collaborating with other labs or clients, you might need share data with them including reports and protocols. ...

  7. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram, which graphically represents the...y-axis from SSC to FSC can alter the positioning and shape of the populations displayed. As the separation ...Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include...

  8. Five Popular Model Organisms

    Type
    Blog Post
    ...by researchers around the globe. These organisms share many genes with humans, are easily maintained in...for the reasons above but because they actually share many biological properties and processes with our...gradually become an important model organism. They share about 70% of their genes with humans and 85% of ...

  9. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...some aspects of their design.  Protein size and shape matters The green fluorescent protein (GFP) from...connexin) has been used as a proxy for dimerization (Shaner et al., 2008 - supplementary figure C). However...PMID: 15247428. PubMed Central PMCID: PMC490022. Shaner, Nathan C., et al. "Improving the photostability...

  10. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...applications, including IF (or IHC or ICC), and will share some of their results and corresponding protocols...may also have tested an antibody in IF and will share their findings either through their publications...poorly in a given context, let your community know! Sharing your experience, positive or negative, will help...

  11. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...between protein abundance and band intensity is an S-shaped curve. Sparse proteins may not be detected at all...Created with BioRender.com. To see why this S-shaped relationship poses problems for proteins of very...this gel is misleading. We can see why on the S-shaped curve. On the protein abundance axis, both blue...

  12. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...(DFHO), a fluorophore modified from DsRed. DFHO shares many structural properties with DFHBI, but its ... Orange Broccoli are derived from Broccoli, and share similar properties to Broccoli despite binding to...PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification...

  13. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...specialized equipment, try finding institutes that offer shared equipment that you can use at a lower cost. Don't...value in the process of creating a budget; you can share your budget with fellow researchers and save them...future. Showing that you are willing to work and share with others will help prove that you will be an ...

  14. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...recombineering can be found in Thomason et al and Sharan et al (a few strains can also be found at Addgene... you used the lambda red recombineering system? Share your tips below! Many thanks to our guest blogger...doi:10.1128/ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL. Recombineering...

  15. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those looking to express high levels... we would love for you to deposit it with us to share it with the scientific community! Good luck and ...86. Crossref, https://doi.org/10.1021/sb500366v. Shaw, William M., et al. “A Multiplex MoClo Toolkit for...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...specific epitope recognized by either antibody was not shared. Tables describe that the antibodies both recognized...on different primary blood leukocytes. The file shared also contains information about the labeling pattern...References Ayoubi, R., Ryan, J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon,...

  17. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...Quick Tip series on YouTube! In this series, we share quick tips to help you master fundamental technical...  Want your quick tips even quicker? We’ll be sharing our favorite parts of every quick tip video on ...

  18. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    ...Data Hub Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with other researchers working...t-shirt.  Our judges, Drs. Connie Cepko, Ian Wickersham,  and Dr. Bobae An will host a Q&A session after...

  19. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ... of today, will stop posting, monitoring, and resharing content on X.  Addgene deeply values our community...knowledge, conversations, and community created and shared on our feed, we will not delete @addgene on X. ...

  20. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...scientific community. Data on CRISPR and synBio plasmid sharing In a recent review on CRISPR technology, for example... over time. You can see these data in the slide share. Addgenies are proud of the role we are playing...
Showing: 221 - 240 of 569 results