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We narrowed to 532 results for: SHI

Showing: 221 - 240 of 532 results
  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...external video recorder and a mic clipped to her shirt. She rolled several long takes, and we cut it up... videos like this would prove to be difficult. Shipping the gear, and walking everyone through the recording...recorder using a lav mic, clipped to the talent’s shirt. But knowing I would have multiple interview subjects...
  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...multiple sources of error (red) influence these relationships and must be addressed with separate controls...accurately quantified In western blotting, the relationship between protein abundance and band intensity... BioRender.com. To see why this S-shaped relationship poses problems for proteins of very different...
  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...non-functional, you may simply introduce a frame-shift mutation. This can be achieved using a restriction...standard ligation reaction resulting in a frame-shifted coding region. It is worth noting that unlike these...specifically designed mutations (not limited to frame shift mutations). Consequently, site directed mutagenesis...
  4. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...GFP to be translated), and second, through a gel shift assay using the PhosTag system (see below). This...phosphate moieties and promotes a robust mobility shift in phosphorylated, but not non-phosphorylated proteins...done using mass spectrometry to identify a mass shift proportional to a single phosphate moiety on the...
  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa, Y., & Konishi, H. (2014). A comparative analysis...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...
  6. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...recombination genes at low temperatures (30-34˚C).  Shifting bacteria to 42˚C for 15 minutes quickly inactivates... is not required for maintenance. A temperature shift to 42˚C not only allows for activation of the red...Department of Laboratory Medicine at the University of Washington. She is particularly interested in science communication...
  7. Using AAV for Neuronal Tracing

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    Blog Post
    ...PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa, S., Takeda, S., Hasegawa, I., and ...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa...
  8. Participate in the Pets of Addgene Scavenger Hunt

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    Blog Post
    ...remotely for the last year to give our lab and shipping teams enough space at HQ and for some of us, that...email from us asking for your mailing address and T shirt size. You have until April 7, 2021 to make your...
  9. Twenty Years of Sharing Science!

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    Blog Post
    ...countries. Just eight days into 2024, we've already shipped 2,078 items to 764 scientists at 375 organizations...different countries. It's safe to say we keep our shipping team busy! But they're not alone: to better serve...
  10. Bringing Renewable Energy to Addgene

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    Blog Post
    ... cannot be washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges can ... striving to set an example of environmental stewardship.  Additional resources on the Addgene blog ...
  11. New Viral Vectors - Spring 2025

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    Blog Post
    ...need for their experiments — and we’ve been able to ship them out in record time.  However, our in-stock ... to the convenience of smaller sizes and quick shipping. We continue to expand the collection to meet ...
  12. MassBioEd Career Exploration Day at Addgene

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    Blog Post
    ...of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their visit for...sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The...
  13. From our Table to Yours: An Inside Look at Lunch at Addgene

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    Blog Post
    ...To-Do list long enough that there is no hope of accomplishing it all. How do we encourage people to take a... Hack Days, and we’ve seen great success at accomplishing “we-should-really-make-time-for-that” type projects...
  14. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ... pizza or pub.” The Cepko Lab celebrates any fellowships, jobs, or paper submissions with dinner and champagne...sharing meals, going on trips, and going to New Hampshire in the summer for the annual lab retreat. How ...
Showing: 221 - 240 of 532 results