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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 541 results for: SHI

Showing: 221 - 240 of 541 results

  2. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...as we obtain more genomic data about plasmids (Shintani et al., 2015). Now, bioinformatics tools help ...10.1016/j.sbi.2011.11.001 Pubmed PMID: 22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial....2015.00242 Pubmed PMID: 25873913 Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid curing method using incompatibility...

  3. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...structure with regard to the Mentor-Singleton relationship. Some of the variability was organic, as some...coffee breaks. While this was not the more intense mentorship experience that happened at ASM Microbe, we connected...Science’s (SACNAS) annual conference all foster mentorship in different but successful ways. Scientific ...

  4. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... the service has grown steadily, helped by a partnership with the University of Pennsylvania’s Vector ...2023. And while viral vectors are not as easy to ship as plasmids, they still have a global reach. From...this drove Addgene to develop new systems, new partnerships, and new platforms, from our early engagement...

  5. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...could have a shipping sprint and all the stories will touch different parts of the shipping code. The following...that the checkout takes the user directly to the shipping step, and no longer takes the user to a terms ...

  6. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes...inversion, and translocation of DNA in a similar fashion as between loxP sites (For details see Cre-lox ...the human brain.     Acknowledgement:Through a partnership with genOway, we are able to distribute materials...

  7. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...come under this ruling, regardless of visa or fellowship status, in addition to certain staff scientists...Department of Labor (for example here and here) pushing for exemptions for postdocs, and lowering of the...set up other institutions (like University of Washington) but as discussed elsewhere, there seems no basis...

  8. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...activities depending on the expertise of the senior leadership and members of their consulting staff. Who would... tasks, and then executing on them in a timely fashion while allowing for creative solutions to emerge...performance, and occasionally being faced with “shifting goal posts” as a part of a case, consulting can...

  9. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...light of the policy, hold onto data longer before publishing. But there are strong incentives to publish such...data initiatives are also taking root. Nature Publishing Group recently launched a publication called ...veracity of collections and interpretation of data, publishing complete data, rather than just a single projection...

  11. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...differences, they had a hard time maintaining their friendships. I put “neutral” in quotations here because I...because that can indicate confusion around... Ownership: Misunderstandings about who is responsible for...for ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork  ...

  12. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...multiple sources of error (red) influence these relationships and must be addressed with separate controls...accurately quantified In western blotting, the relationship between protein abundance and band intensity... BioRender.com. To see why this S-shaped relationship poses problems for proteins of very different...

  13. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...external video recorder and a mic clipped to her shirt. She rolled several long takes, and we cut it up... videos like this would prove to be difficult. Shipping the gear, and walking everyone through the recording...recorder using a lav mic, clipped to the talent’s shirt. But knowing I would have multiple interview subjects...

  14. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...non-functional, you may simply introduce a frame-shift mutation. This can be achieved using a restriction...standard ligation reaction resulting in a frame-shifted coding region. It is worth noting that unlike these...specifically designed mutations (not limited to frame shift mutations). Consequently, site directed mutagenesis...

  15. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...GFP to be translated), and second, through a gel shift assay using the PhosTag system (see below). This...phosphate moieties and promotes a robust mobility shift in phosphorylated, but not non-phosphorylated proteins...done using mass spectrometry to identify a mass shift proportional to a single phosphate moiety on the...

  16. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa, Y., & Konishi, H. (2014). A comparative analysis...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

  18. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...recombination genes at low temperatures (30-34˚C).  Shifting bacteria to 42˚C for 15 minutes quickly inactivates... is not required for maintenance. A temperature shift to 42˚C not only allows for activation of the red...Department of Laboratory Medicine at the University of Washington. She is particularly interested in science communication...

  19. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    ...remotely for the last year to give our lab and shipping teams enough space at HQ and for some of us, that...email from us asking for your mailing address and T shirt size. You have until April 7, 2021 to make your...

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa, S., Takeda, S., Hasegawa, I., and ...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa...
Showing: 221 - 240 of 541 results