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We narrowed to 651 results for: SIM

Showing: 221 - 240 of 651 results

  1. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...minigenomes are incapable of producing infectious virus (similar to lentiviral vectors) because they do not encode...components of the Zaire ebola virus minigenome system Similar to other RNA viruses, EBOV brings its own RNA-dependent...studied independently of other viral proteins simplifying the interpretation of results. This system can...

  2. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...done annually - they are fan favorites! 6. Keep it simple This may be an obvious one, but don’t make things...really! We've had plenty of events that included a simple corn hole set and a cooler. Chances are one of ...company’s intranet. Don’t forget about the power of simple word of mouth - be sure to bring up your event ...

  3. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...pBAD-bARGSer-AxeTxe, gas vesicle expression in E. coli is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L...improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via regular intraperitoneal...a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP...

  4. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ... and Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase ...excision, inversion, and translocation of DNA in a similar fashion as between loxP sites (For details see ...codon optimization resulted in FLPO, which shows similar efficiency to Cre (Kranz et al. 2010). These differences...

  5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...Just like analysis pipelines for DNA that group similar sequences together in bins, we thought that binning...mentor, as well as having a cohort experiencing similar situations to themselves, allowed the participants...greater effort in the future to match people within similar fields (ASM Microbe has eight tracks), something...

  6. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...implemented a policy of never rejecting an idea simply because it's not how things were done in the past...’t have to be perfect or even good. The goal is simply to throw out ideas.  When your five minutes are...If you’re in a time crunch, your break can be as simple as a few minutes of meditation or a walk to the...

  7. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Immobilization is the key to immunoprecipitation and simply refers to the process of anchoring an antibody,...applications Oftentimes, an IP-appropriate antibody simply isn’t available for a specific target. In cases...binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used to isolate proteins ...

  8. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens and how best ...off-target cutting patterns in genome-wide screens similar to patterns seen in single-guide studies? Many ... Short gRNAs have lower off-target cutting and similar on-targeting cutting as full-length gRNAs Lastly...

  9. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...less risk averse. Similar to the elevated plus maze, the light-dark box is a simple device comprised of...downregulating expression of miR124a achieved a similar improvement in the forced swim test.  Another brain...

  10. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust strategy uses a ...tail. Compared to the opaque mouse embryo, where similar processes occur over days inside the mother, the... Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2...

  11. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...tobacco, Agrobacterium infiltration into leaves is a simple and efficient way to overexpress a gene and evaluate...abaxial site (lower surface) of the leaf. This is a simple and easy technique to implement. Success might ... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

  13. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...sgRNAs. Its RNA-guided nature makes CRISPRa much simpler to implement in genome-wide screens over cDNA libraries... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...PMCID: PMC6412672. Zhou, Haibo, et al. "In vivo simultaneous transcriptional activation of multiple genes...

  14. Hot Plasmids - August 2020

    Type
    Blog Post
    ...//doi.org/10.1101/160374 Nanobodies- there's a simple method for that! By Michelle Cronin The benefits...Kimiko Hara, and Kazuo Umemura have described a simple method which alleviates the inclusion body problem...problem and makes nanobody production in E.coli as simple as following a DNA miniprep protocol!  First, the...

  15. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...identify a large variety of related bacteria with similar Cas systems. Some of these Cas systems have enzymes...of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13 systems in a range of mammalian... matter – the mutated copy could be replaced by simply dragging and dropping in a functional copy of the...

  16. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...transfected into human cells. From this result one could simply conclude that the shRNA didn’t work, as the expression...level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated... controls are negative controls that produce a similar product, but do not target an endogenous gene in...

  17. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...efficiently trawled their own data? A distinct (perhaps similarly impoverished lab) might apply different or novel...although it might be argued various authors have simply chosen to submit elsewhere). The majority of the...types? And how long should data be archived? Simplifying data access Some of the above mentioned issues...

  18. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...expression mRNA Mammalian expression promoter from the simian vacuolating virus 40 Constitutive  May include ...

  19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...edit your gene of choice. These vectors are very similar, both with polylinkers for the addition of gene-specific...at once. Therefore, applications that require simultaneous modification of all alleles of a gene, or which...which require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based...

  20. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...fastest test that has been developed so far.  A similar method has been optimized to detect the SARS-CoV...and will not give a positive result for highly similar viruses. Unlike Cas12a, Cas13a does not require...illuminator or UV light illuminator. Approach: Highly similar to the SHERLOCK diagnostic test, this test varies...
Showing: 221 - 240 of 651 results