... filters you do not need to start a new search. Simply click the ‘X’ next to any filter to clear it. ... Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental... plasmids that may be difficult to define for a simple search. These pages provide helpful information...

...done annually - they are fan favorites! 6. Keep it simple This may be an obvious one, but don’t make things...really! We've had plenty of events that included a simple corn hole set and a cooler. Chances are one of ...company’s intranet. Don’t forget about the power of simple word of mouth - be sure to bring up your event ...

...increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and ... not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color labeling is ...PubMed PMID: 28671695. 2. Deverman BE, Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants...

...pBAD-bARGSer-AxeTxe, gas vesicle expression in E. coli is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L...improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via regular intraperitoneal...a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP...

...less risk averse. Similar to the elevated plus maze, the light-dark box is a simple device comprised of...downregulating expression of miR124a achieved a similar improvement in the forced swim test.  Another brain...

...transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust strategy uses a ...tail. Compared to the opaque mouse embryo, where similar processes occur over days inside the mother, the... Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2...

...tobacco, Agrobacterium infiltration into leaves is a simple and efficient way to overexpress a gene and evaluate...abaxial site (lower surface) of the leaf. This is a simple and easy technique to implement. Success might ... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

...regions. PHP.eB, an enhanced version of PHP.B, shows similar transduction efficiency, but at a log lower dose...pattern in the mouse brain.  Both labs then took similar routes to verify LY6A’s role as a receptor for ...transduce cells. The Wilson Lab generated results similar to the Deverman Lab when they looked at LY6A protein...

...sgRNAs. Its RNA-guided nature makes CRISPRa much simpler to implement in genome-wide screens over cDNA libraries... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...PMCID: PMC6412672. Zhou, Haibo, et al. "In vivo simultaneous transcriptional activation of multiple genes...

...Just like analysis pipelines for DNA that group similar sequences together in bins, we thought that binning...mentor, as well as having a cohort experiencing similar situations to themselves, allowed the participants...greater effort in the future to match people within similar fields (ASM Microbe has eight tracks), something...

...//doi.org/10.1101/160374 Nanobodies- there's a simple method for that! By Michelle Cronin The benefits...Kimiko Hara, and Kazuo Umemura have described a simple method which alleviates the inclusion body problem...problem and makes nanobody production in E.coli as simple as following a DNA miniprep protocol!  First, the...

...identify a large variety of related bacteria with similar Cas systems. Some of these Cas systems have enzymes...of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13 systems in a range of mammalian... matter – the mutated copy could be replaced by simply dragging and dropping in a functional copy of the...

...implemented a policy of never rejecting an idea simply because it's not how things were done in the past...’t have to be perfect or even good. The goal is simply to throw out ideas.  When your five minutes are...If you’re in a time crunch, your break can be as simple as a few minutes of meditation or a walk to the...

...Immobilization is the key to immunoprecipitation and simply refers to the process of anchoring an antibody,...applications Oftentimes, an IP-appropriate antibody simply isn’t available for a specific target. In cases...binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used to isolate proteins ...

...for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens and how best ...off-target cutting patterns in genome-wide screens similar to patterns seen in single-guide studies? Many ... Short gRNAs have lower off-target cutting and similar on-targeting cutting as full-length gRNAs Lastly...

... and Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase ...excision, inversion, and translocation of DNA in a similar fashion as between loxP sites (For details see ...codon optimization resulted in FLPO, which shows similar efficiency to Cre (Kranz et al. 2010). These differences...

...transfected into human cells. From this result one could simply conclude that the shRNA didn’t work, as the expression...level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated... controls are negative controls that produce a similar product, but do not target an endogenous gene in...

...efficiently trawled their own data? A distinct (perhaps similarly impoverished lab) might apply different or novel...although it might be argued various authors have simply chosen to submit elsewhere). The majority of the...types? And how long should data be archived? Simplifying data access Some of the above mentioned issues...

...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...expression mRNA Mammalian expression promoter from the simian vacuolating virus 40 Constitutive  May include ...

...the immune response. The second strategy is the simple removal of the HIV-1 genome from infected cells...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...