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Addgene

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We narrowed to 466 results for: Sele

Showing: 221 - 240 of 466 results

  1. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...transfected. Alternatively, you can use a selection marker to select transfected cells (for example, plasmid...your HR template has a selection marker such as puromycin, you can (also) select for the positive colonies...sequence. Because the 'NGG' of the PAM is used to select your genomic target, you need to make sure the ...protospacer followed by PAM. Click here for a target selection tool that should be helpful in minimizing or testing...clone in your target guides, test run them and then select the best guide to co-transfect in HR donor. Alternatively.../abstract/S0092-8674(13)01015-5 As for target selection, we usually pick 3-6 guides around the region ... might still be okay if you are introducing a selection marker gene. The homology arms should be no more...

  13. CRISPR Plasmids - Plants

    Type
    Collection
    ...Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression... efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically...strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase...repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase... RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

  14. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI ... Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI...Xenopus. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

  17. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...significantly improve the transformation efficiency).  5. Select and Analyze 10 White or Light Blue Colonies: You...

  20. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically...strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase.... ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...
Showing: 221 - 240 of 466 results