We narrowed to 333 results for: cki

1. Viral Vectors 101: Biosensors

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   Blog Post

   ...biosensors discussed are genetically encoded, and luckily, we have pre-designed plasmids for these systems...

2. Viral Vectors 101: Viruses as Biological Tools

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   Blog Post

   ...replicate and spread. Instead, viruses replicate by hijacking the host’s system to produce more virus and continue...

3. Plasmids 101: Screens vs. Selections

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   Blog Post

   ... screens are similar to CRISPR, but instead of knocking out the genes of interest (or the whole genome...

4. Hot Plasmids - February 2022

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   Blog Post

   ..., including live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting...

5. Sweating the Small Stuff: Details in the Lab

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   Blog Post

   ...running through the experiment in my head, double-checking details, or just taking a moment to relax. During...

6. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   ...purposes or for diagnostics, we suggest double checking to make sure your results will not be affected...

7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   ...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...

8. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ...Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing...

9. Dos & Don'ts When Publishing a Scientific Manuscript

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   Blog Post

   ...journals as possible at the same time. Take your time picking the appropriate journal and explain in your cover...

10. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    ... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...

11. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ... interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces green fluorescence...

12. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...in some applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...

13. CRISPRainbow and Genome Visualization

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    Blog Post

    ...rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However...

14. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    ... postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...

15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post

    ...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and...

16. Addgene: The First Twenty Years

    Type

    Blog Post

    ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...

17. Antibodies 101: Fab Fragments

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    Blog Post

    ...fragment. But this bivalency may cause problems for blocking purposes, since the fragment could bind not only...

18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ... our biosensor repository as we find ourselves lacking several crucial biosensors that would allow us ...

19. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    ...and systematically mutated it. Using a robotic picking approach to select cells with variant characteristics...

20. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ... experiment! As a starting point, we recommend checking the literature and our AAV data hub to see if ...