Addgene

We narrowed to 330 results for: cki

Showing: 221 - 240 of 330 results

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The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

Type

Blog Post

...significant result that may be the result of noise, p-hacking, or unclear methods. Many thanks to our guest ...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing...
Dos & Don'ts When Publishing a Scientific Manuscript

Type

Blog Post

...journals as possible at the same time. Take your time picking the appropriate journal and explain in your cover...
Kiran Musunuru on the Newest TALEN Genome-Editing System

Type

Blog Post

... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

...and systematically mutated it. Using a robotic picking approach to select cells with variant characteristics...
Viral Vectors 101: An Introduction to AAV

Type

Blog Post

... experiment! As a starting point, we recommend checking the literature and our AAV data hub to see if ...
The time and cost required to make a plasmid

Type

Blog Post

... the traditional PCR/digest/ligate methods and picking the right one can save you time if you’re working...
Addgene: The First Twenty Years

Type

Blog Post

...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...
Antibodies 101: Fab Fragments

Type

Blog Post

...fragment. But this bivalency may cause problems for blocking purposes, since the fragment could bind not only...
CRISPRainbow and Genome Visualization

Type

Blog Post

...rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However...
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Type

Blog Post

... postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Type

Blog Post

...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and...
Lighting Up Cell Signaling with Photoswitchable Kinases

Type

Blog Post

... interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces green fluorescence...
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Type

Blog Post

...in some applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...
Antibodies 101: Affinity Reagents

Type

Blog Post

...nanobodies, are derived from a camelid antibody lacking a light chain, called a heavy chain antibody (Muyldermans...
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

Type

Blog Post

...surface display vectors to improve expression and trafficking of these biosensors. Figure 1: Schematic...
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Type

Blog Post

...between them, could affect the properties or trafficking of the fusion protein (Kimple et al., 2013). ...
Supporting Rare Disease Research with the 2020 BeHEARD Awards

Type

Blog Post

... our biosensor repository as we find ourselves lacking several crucial biosensors that would allow us ...
Transferable Skills Guide: Cross-team Communication

Type

Blog Post

...and racks meant we had fewer errors in our LIMS tracking system. Though you might not have to communicate...

Showing: 221 - 240 of 330 results

Results per page:

The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

CRISPR 101: Non-Homologous End Joining

Dos & Don'ts When Publishing a Scientific Manuscript

Kiran Musunuru on the Newest TALEN Genome-Editing System

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Viral Vectors 101: An Introduction to AAV

The time and cost required to make a plasmid

Addgene: The First Twenty Years

Antibodies 101: Fab Fragments

CRISPRainbow and Genome Visualization

Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Lighting Up Cell Signaling with Photoswitchable Kinases

Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Antibodies 101: Affinity Reagents

Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Supporting Rare Disease Research with the 2020 BeHEARD Awards

Transferable Skills Guide: Cross-team Communication

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