We narrowed to 333 results for: cki

1. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ...significant result that may be the result of noise, p-hacking, or unclear methods. Many thanks to our guest ...

2. A Novice's Perspective on Neuroscience - SfN2017

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   Blog Post

   ...people ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around...

3. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   ...purposes or for diagnostics, we suggest double checking to make sure your results will not be affected...

4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...

5. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ...Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing...

6. Dos & Don'ts When Publishing a Scientific Manuscript

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   Blog Post

   ...journals as possible at the same time. Take your time picking the appropriate journal and explain in your cover...

7. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   Blog Post

   ... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...

8. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   Blog Post

   ...and systematically mutated it. Using a robotic picking approach to select cells with variant characteristics...

9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

   ... our biosensor repository as we find ourselves lacking several crucial biosensors that would allow us ...

10. Antibodies 101: Affinity Reagents

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    Blog Post

    ...nanobodies, are derived from a camelid antibody lacking a light chain, called a heavy chain antibody (Muyldermans...

11. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

    ...surface display vectors to improve expression and trafficking of these biosensors.   Figure 1: Schematic...

12. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ...between them, could affect the properties or trafficking of the fusion protein (Kimple et al., 2013). ...

13. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ... interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces green fluorescence...

14. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...in some applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...

15. CRISPRainbow and Genome Visualization

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    Blog Post

    ...rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However...

16. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    Blog Post

    ... postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...

17. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ... experiment! As a starting point, we recommend checking the literature and our AAV data hub to see if ...

18. The time and cost required to make a plasmid

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    Blog Post

    ... the traditional PCR/digest/ligate methods and picking the right one can save you time if you’re working...

19. Addgene: The First Twenty Years

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    Blog Post

    ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...

20. Antibodies 101: Fab Fragments

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    Blog Post

    ...fragment. But this bivalency may cause problems for blocking purposes, since the fragment could bind not only...