We narrowed to 310 results for: construct

1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   ...at the plasma membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate...

2. Corralling and Cornering the Goldilocks Antibody

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   ...sequencing to turn mAb clones into expression constructs to be conveniently modified and engineered.   ...

3. Plasmids 101: Terminators and PolyA signals

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   ...terminator may be desired for multi-cistronic constructs where high termination efficiency is necessary...

4. The Materials Science of Optogenetics Experiments

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   ...Central PMCID: PMC3138296. 2. Sparta DR, et al. Construction of implantable optical fibers for long-term ...

5. New England Biolabs Cell-Imaging Plasmid Collection

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   ..., and for internal or surface proteins Single constructs are compatible with multiple applications (different...

6. Distribution to Industry

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   ...standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel...

7. Zinc Finger Consortium: Zinc Finger Arrays

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   ... ZFNs targeted to a site within that gene. To construct ZFN expression vectors, DNA encoding zinc finger...

8. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   ...sites (Geertsma, 2013). Once the phagemids are constructed, they are used to produce a phage library consisting...

9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find fluorescent proteins...

10. Hot Plasmids and Viral Preps - May 2021

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    ...vectors and DNA modules for assembly of CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized...

11. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...CRISPR/Cas9 induced break. Learn more about these constructs and the cell lines they’ve been used to create...

12. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...and invite you to make any fluorescent protein constructs you create in your own work available to the ...

13. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ... a proof of principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter...

14. The Scientific Conference Poster Session: Tips for Success

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    ... they can digest what you’re saying, provide constructive criticism, and ask thought-provoking questions...

15. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...PGK promoter) and U6 promoter-driven guide RNA constructs, pAdSh.U6.gRNAS1 and pAdSh.U6.gRNAGFP, and also...

16. Addgene's Cancer Collection Pages Connect the Research Community

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    ...Its major appeal is that it enables the simple construction of vectors to study the function of any of these...

17. Modular Cloning Guide

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    ... for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high...a growing number of libraries containing pre-constructed standardized modules. In the first assembly step...vectors permits the creation of even more complex constructs constrained only by the ability of E. coli to...standardized parts to create eukaryotic multigene constructs. MoClo Plant Parts Kit Plant Expression Nicola... for hierarchical assembly of plant multigene constructs. MoClo Plant Parts III: Transformation & Genome...containing several cassettes and generate multi-construct transgenic plants. GreenGate 2.0 Toolkit Plant... (Lohmann) to create plasmids with multi-gene constructs for plant transformation. GoldenBraid 2.0 Kit...

18. The Pleiades Promoter Project

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    ... Additional information is available for each construct and can be accessed using the links to view individual...Pleiades MiniPromoters MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A pEMS1142 EGFP/NLS Ple3 ADORA2A...

19. Hot Plasmids: Summer 2025

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    ...team characterized and deposited a variety of constructs for visualizing cellular components (nucleus,...

20. Production of Virus in Insect Versus Mammalian Cells

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    ...plasmids — your transfer plasmid and the baculovirus construct, called a bacmid. Like AAV and lentiviral packaging...