We narrowed to 306 results for: construct

...detailed information on cloning procedures used to construct the deposited plasmids. Molecules: We synthesized...

...modifications for proper protein function. Most DNA constructs designed for mammalian expression utilize viral...

...circular plasmid via PCR. Typically, to make these constructs, developmental biologists have to extract RNA...

...at the plasma membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate...

...terminator may be desired for multi-cistronic constructs where high termination efficiency is necessary...

...Central PMCID: PMC3138296. 2. Sparta DR, et al. Construction of implantable optical fibers for long-term ...

...linearized with the restriction enzyme PmeI . This construct is then transformed into AdEasier-1 cells (Addgene...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293...expressing E1 are transfected with the linearized construct, and rAdV vectors can be harvested about 7–10 ...purposes. The pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene...adding 2.7 kb of packaging space. However, these constructs must be transfected into HER911 E4+ cells for...Ad2 are the most commonly used for rAdV vector construction, due to their high gene transfer efficiency....

...Addgene's viral vector packaging service. Browse constructs currently available in lentiviral format....

...typical titers, though actual yields may vary by construct. Size Quantity Total Volume Minimum Titer Typical...

...background signal (especially for some older split-GFP constructs or certain cell environments). Also consider ...

...vectors and DNA modules for assembly of CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized...

...sites (Geertsma, 2013). Once the phagemids are constructed, they are used to produce a phage library consisting...

...FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find fluorescent proteins...

...and invite you to make any fluorescent protein constructs you create in your own work available to the ...

... a proof of principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter...

...Its major appeal is that it enables the simple construction of vectors to study the function of any of these...

...CRISPR/Cas9 induced break. Learn more about these constructs and the cell lines they’ve been used to create...

...PGK promoter) and U6 promoter-driven guide RNA constructs, pAdSh.U6.gRNAS1 and pAdSh.U6.gRNAGFP, and also...

... they can digest what you’re saying, provide constructive criticism, and ask thought-provoking questions...

...Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control expression of your...Return to Top Transactivators (tTA or rtTA) Find a construct that expresses the transactivator for your tetracycline...