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We narrowed to 495 results for: control

Showing: 221 - 240 of 495 results
  1. Fluorescent Protein Guide

    Type
    Collection
    ...Optogenetics Plasmids Use light to activate, inhibit, and control molecular pathways or cells. Chemogenetics Guide...
  2. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...reference 3. Production, amplification, and quality control of 2nd generation AdV     AdV Production in HEK293...reference material should be used as an internal control (ATCC#VR-1516) until the assay is validated. Infectious...
  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...mitigated by using low doses and running appropriate controls (Jendryka et al., 2019), they have resulted in...channel, the PSAM acts as a gating mechanism to control ion flux. The system allows you to directly manipulate...
  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...necessary gRNAs, but it would not be possible to control which gRNA becomes complexed with which variant...represents a straightforward means of exerting tight control over Cas9 nuclease activity while eliminating the...
  5. Tips for arabidopsis transformation

    Type
    Blog Post
    ...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ...
  6. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off transactivator (tTA...fluorescent tag-then-express system that allows for controlled induction of multi-color labeling of neurons ...
  7. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...check for GUS-like activity of your tissue. Run a control with Agrobacterium lacking the GUS gene since plants...promoter activity, for example. Useful positive controls could include: pSB96 - pL2_pSB90_2x35S::fLUC-I...
  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... period of two weeks. After this time, quality control assays showed that the majority of antibodies are...Protein aggregation - Mechanisms, detection, and control. Int J Pharm. 2018 Oct 25;550(1-2):251-268. doi...
  9. Hot Plasmids - November 2023

    Type
    Blog Post
    ... enzyme, Lee and Cheah et al. achieved precise control over when and where the enzyme is active, drastically...light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells. Nature Methods...
  10. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...a low multiplicity of infection (MOI). MOI is controlled by viral titer, so you’ll need to measure your...Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend determining the functional...
  11. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014)...efficient homology-directed repair and termperature-controlled genome editing. Nat. Com., 8(2024), (2017). 10.1038...
Showing: 221 - 240 of 495 results