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We narrowed to 494 results for: control

Showing: 221 - 240 of 494 results

  1. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...and will play a different role so her ability to control the event is what is important. Choose 4 panelists...it (lapel mics are best). The moderator takes control It is the moderator's job to prevent anyone from...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted to LacZ, plus luciferase...within the mouse genome. This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone...targeting the neuronal-specific gene NeuN. As a control they designed an sgRNA targeting LacZ, which is...

  4. All Antibodies

    Type
    Collection
    ...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...reference 3. Production, amplification, and quality control of 2nd generation AdV     AdV Production in HEK293...reference material should be used as an internal control (ATCC#VR-1516) until the assay is validated. Infectious...

  8. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...mitigated by using low doses and running appropriate controls (Jendryka et al., 2019), they have resulted in...channel, the PSAM acts as a gating mechanism to control ion flux. The system allows you to directly manipulate...

  9. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...necessary gRNAs, but it would not be possible to control which gRNA becomes complexed with which variant...represents a straightforward means of exerting tight control over Cas9 nuclease activity while eliminating the...

  11. Chemogenetics Plasmids

    Type
    Collection
    ...specific small molecules, allowing a scientist to control signaling within cells expressing these engineered...

  13. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...check for GUS-like activity of your tissue. Run a control with Agrobacterium lacking the GUS gene since plants...promoter activity, for example. Useful positive controls could include: pSB96 - pL2_pSB90_2x35S::fLUC-I...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... period of two weeks. After this time, quality control assays showed that the majority of antibodies are...Protein aggregation - Mechanisms, detection, and control. Int J Pharm. 2018 Oct 25;550(1-2):251-268. doi...

  15. Tips for arabidopsis transformation

    Type
    Blog Post
    ...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ...

  18. Hot Plasmids - November 2023

    Type
    Blog Post
    ... enzyme, Lee and Cheah et al. achieved precise control over when and where the enzyme is active, drastically...light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells. Nature Methods...

  19. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...a low multiplicity of infection (MOI). MOI is controlled by viral titer, so you’ll need to measure your...Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend determining the functional...

  20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off transactivator (tTA...fluorescent tag-then-express system that allows for controlled induction of multi-color labeling of neurons ...
Showing: 221 - 240 of 494 results