We narrowed to 494 results for: control
-
TypeBlog Post...expression in C.elegans. In this system a promoter controls the expression of a GAL-4 driver that can bind...split intein for refined spatiotemporal transgene control in Caenorhabditis elegans." Proceedings of the ...
-
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...infect target tissues. Improved specificity through control of gene expression and the selection of specific...CRISPR-AAV vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post.... Site-specific selfish genes as tools for the control and genetic engineering of natural populations....Fitz-Earle, M., Holm, D. G. & Suzuki, D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...and will play a different role so her ability to control the event is what is important. Choose 4 panelists...it (lapel mics are best). The moderator takes control It is the moderator's job to prevent anyone from... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post...two posts. Flow Compensation and Flow Cytometry Controls Priyamvada Prathima, who wrote a helpful guide... -
Chemogenetics Plasmids
TypeCollection...specific small molecules, allowing a scientist to control signaling within cells expressing these engineered... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...expresses the VSV-G envelope protein under the control of the powerful mammalian CMV promoter. Envelope... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post...many steps in the viral production and quality control process to improve both the yield and quality of... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...reference 3. Production, amplification, and quality control of 2nd generation AdV AdV Production in HEK293...reference material should be used as an internal control (ATCC#VR-1516) until the assay is validated. Infectious... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...necessary gRNAs, but it would not be possible to control which gRNA becomes complexed with which variant...represents a straightforward means of exerting tight control over Cas9 nuclease activity while eliminating the... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...mitigated by using low doses and running appropriate controls (Jendryka et al., 2019), they have resulted in...channel, the PSAM acts as a gating mechanism to control ion flux. The system allows you to directly manipulate... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...question. You can see that we have the beginning of control around the central dogma. We have the beginnings...priority; we need the keyboards and displays for control and visualization. Addgene: What do you envision... -
Tips for arabidopsis transformation
TypeBlog Post...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...average of 4 sgRNAs per gene and 1,000 non-coding control sgRNAs. This library was designed to improve on-target... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...organisms to control their movements and behavior. These functions and others are controlled by collections... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post... AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off transactivator (tTA...fluorescent tag-then-express system that allows for controlled induction of multi-color labeling of neurons ... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...provided a major toehold into understanding how genes control biology (a fact celebrated by the Nobel prize committee...into adults and explain how signaling pathways control this process. Additional zebrafish applications... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...check for GUS-like activity of your tissue. Run a control with Agrobacterium lacking the GUS gene since plants...promoter activity, for example. Useful positive controls could include: pSB96 - pL2_pSB90_2x35S::fLUC-I... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post... period of two weeks. After this time, quality control assays showed that the majority of antibodies are...Protein aggregation - Mechanisms, detection, and control. Int J Pharm. 2018 Oct 25;550(1-2):251-268. doi... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014)...efficient homology-directed repair and termperature-controlled genome editing. Nat. Com., 8(2024), (2017). 10.1038...