...incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused...

...as wild lettuce. Aside from their use as food, infusions of L. taraxacifolia leaves are widely used to ...

...abundance of a protein variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal...

...environmental conditions (such as temperature, pH, fusion protein, etc...).  There are many online guides...

... functional. Attaching the fluorescent protein fusions allowed us to image R bodies inside cells, and ...

...catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case a deaminase...

...molecular and cell biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques...

...buoyancy. These proteins allow dissolved gases to diffuse in and out but prohibit water from entering their...

...Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical...

... al. saw great results with CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293...

...with a plasmid containing a fluorescent protein (fusion) there is huge variation in the fluorescence intensities...

...clarity. But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit,...

...human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin...

...systems with limited packaging capacity. Still confused about the various tRNA expression strategies? ...

...journals and publishers to choose from, it gets very confusing for scientists writing research articles. What...

...CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers...Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine ...Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help visualize...

... is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this fusion protein...examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy...Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various organelles...organelles or subcellular structures. You should fuse your protein of interest with a fluorescent protein that...Membrane CARMIL2 BH domain tdTomato John Cooper *Fusions to other FPs can be found by searching the complete...Microtubules ensconsin mCherry William Bement *Fusions to other FPs can be found by searching the complete...Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions to other FPs can be found by searching the complete...

...so you end up acting it out in front of a very confused group of visiting Chinese undergrads. You get ...

...get high volumes of plasmid requests can end up refusing such requests in order to preserve time and money...

... is a naturally occurring RNase composed of the fusion of several Cas7 and Cas11 domains. Cas7-11 cuts...