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  1. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ... of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang... (ex: antibiotic selection and auxotrophy) and negative selection (ex: toxin-antitoxin systems and sacB...101: Transformation, Transduction, Bacterial Conjugation, and Transfection by Alyssa Cecchetelli The ...
  2. Management for Scientists: Seeking Feedback

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    Blog Post
    ... is no way a manager can be efficient without delegating and empowering the team to solve problems together...together (tactics for effective delegation will be covered in a future post in this series). Members ...at formal communications events or at informal gatherings, like at lunch. Many experts recommend using ...
  3. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...the inside of the neuron more positive or more negative.  Neuronal stimulation: Depolarization When the...Conversely, when the inside of the neuron becomes more negative it becomes less likely to fire and is said to ...hyperpolarized. The chloride pump NpHR brings in negative chloride ions which hyperpolarize the cell. It...
  4. Plasmids 101: Antibiotic Resistance Genes

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    Blog Post
    ...we'll focus primarily on antibiotics against Gram negative bacteria. In future posts, we'll detail selection...ribosomal subunit; inhibits protein synthesis (elongation step) Bacteriostatic 10 ug/mL Chloramphenicol...inactivates or completely removes (in the case of Gateway cloning) one cassette. Counterselection allows ...
  5. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... positive), with these puncta turning red (GFP negative/RFP positive) upon fusion of an autophagosome ...RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated to phosphatidylethanolamine (PE) and recruited...autophagy. The RFP-LC3∆G, however, can not be conjugated to PE due to a C-terminal glycine deletion, so...
  6. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post
    ...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...
  7. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    Blog Post
    .... These studies used SMRT sequencing, TA-based ligation and tagmentation-based methods for AAV genome ...works for self-complementary AAVs and the TA-based ligation and tagmentation preparation approaches described...AAV genome is single-stranded and both TA-based ligation and tagmentation require a double-stranded template...
  8. RUBY-Red Siliques

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    Blog Post
    ...next generation the CRISPR construct needs to be segregated away to prevent continued production of new alleles... are identified by a phenotype caused by the segregating mutation. Selecting these plants is a learned...integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could be achieved much...
  9. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...product and vector ligate; however, it can potentially rebind and nick the newly ligated DNA. Salt helps ...an extremely large insert. Since the standard ligation reaction is fairly quick, make sure you stay organized...
  10. Plasmid Cloning by PCR

    Type
    Blog Post
    ...NotI sites to Your Gene of Interest (YGOI) for ligation into a recipient plasmid. Designing primers for... In our example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid. Remember to...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...
  11. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies biofilm formed by Pseudomonas...compounds on these proteins will be tested. Investigating changes in neuronal activity after ischemic ...Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular and cellular etiopathomechanisms...
  12. K. phaffii: Rising to the Occasion

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    Blog Post
    ... Facultative anaerobe Facultative anaerobe Obligate aerobe Number of chromosomes 16 3 4 ... is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic yeast, meaning...DOI: 10.3892/br.2017.941 Bernauer L., et al., Komagataella phaffi as Emerging Model Organisms in Fundamental...
  13. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...conformational changes in proteins? How can information propagate through a structured material over a long distance...amplified tens or hundreds of thousands of times by propagating through an enormous lattice of identical subunits...
  14. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...challenging. How do you prepare for this shift and navigate the pitfalls? Here's what you need to know.   ...lead to frustration. Clinging to technical tasksDelegation can feel unnatural for those accustomed to ...Type Indicator or DISC assessments can help you navigate team dynamics effectively and foster a cohesive...
  15. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...False positives in original studies and false negatives in replications are unavoidable, but can be minimized...and hypothesis generation, in which data are investigated to look for unexpected trends or differences...possible null is really a true null and not a false negative. Therefore, they will place special emphasis on...
  16. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...addition, we worked with many collaborators to aggregate a sequence-confirmed series of clones corresponding...part of our Genetic Pathway Reference Set. These Gateway-compatible entry clones are the product of many...described in this paper. Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic...
  17. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...and the subsequent product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt...maintained. Recombination efficiency is high enough to negate the need for selection usually required in recombineering... Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our...
  18. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...pigment precipitation, enhances the blue color of negative colonies, and allows for better differentiation...also lead to a white colony. And also false negatives: These are rare, but if a small fragment is inserted...example is the ccdB gene system commonly used in Gateway® Cloning. There are also methods to select for ...
  19. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...geographical regions When we originally started gathering this information, many assumed that those living...location of the institution. Continuing efforts to gather information and expedite change We are continuing...advocating for and with junior scientists, to help them navigate the scientific enterprise and promote their voices...
Showing: 221 - 240 of 625 results