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Human sgRNA library Gattinara in pRDA_118 backbone

Pooled Library

#136986

Purpose

Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brunello human genome-wide library.

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only
IV-GAT

Plasmid

#43766

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

IV-GAT

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pDONR223_GATA3_WT

Plasmid

#81902

Purpose

Gateway Donor vector containing GATA3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GATA3 (GATA3 Human)

Use

Gateway entry vector

Mutation

V3A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MAC_N_GATA4

Plasmid

#167758

Purpose

Expresses GATA4 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

GATA4 (GATA4 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Gata4

Plasmid

#72690

Purpose

Doxycyline regulated expression of Gata4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 4 (Gata4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_GATA6_P2A_Hygro_Barcode

Plasmid

#120445

Purpose

Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_GATA4_P2A_Hygro_Barcode

Plasmid

#120444

Purpose

Barcoded lentiviral vector to express GATA4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Gata6

Plasmid

#72694

Purpose

Doxycyline regulated expression of Gata6 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 6 (Gata6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pXNubWtgate

Plasmid

#105080

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubI

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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hGATA-1 WT

Plasmid

#118352

Purpose

This plasmid expresses human GATA-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMetYCgate

Plasmid

#105074

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

Cub-PLV

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-16

Plasmid

#160716

Purpose

T-nos Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

nopaline synthase terminator (T-nos) bordered by BsaI restriction sites

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNubWtXgate

Plasmid

#105079

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubI

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-1

Plasmid

#160701

Purpose

derivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

lacZ

Use

Synthetic Biology

Mutation

LacZ bordered by BsaI restrction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-5

Plasmid

#160705

Purpose

Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-2

Plasmid

#160702

Purpose

gpdh promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

gpdh

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_GATA1_P2A_Hygro_Barcode

Plasmid

#120442

Purpose

Barcoded lentiviral vector to express GATA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PB-Gata2

Plasmid

#60665

Purpose

PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Gata2 (Gata2 Rat)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGATOR

Plasmid

#64102

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector

Depositor

Bill Skarnes

Article

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pGAT2

Plasmid

#112588

Purpose

Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-GST fusions

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His-GST

Expression

Bacterial

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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