We narrowed to 263 results for: his-tag

...observed in the kidneys at a specific developmental stage you could infer that the gene which GFP was monitoring...throughout an organism’s genome. These reporters have historically been used extensively in fly, mouse, and yeast...Development-specific genes – if a gene functions at multiple stages in development, its depletion may result in lethality...

...tuned” with mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact...enormous lattice of identical subunits. A brief history of R bodies R bodies have been studied for many...

...graduate with my BSc in Anatomy, the University of Otago had established the Centre for Science communication...flourish. I’ve never forgot about all the anxious, whispered questions from friends and classmates back in ...Department of Science Communication at the University of Otago. Her research focuses on misinformation about sexual...

...characterized orthologs from “Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed...immunogenicity. But these studies also identified other advantages, including Cas13d-specific regulatory proteins...Plasmids Check out our CRISPR Guide Read our CRISPR History  ...

...of the biosensors used in research are very sophisticated, but at their core they can all be boiled down...Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific sensors designed...good thing, though! Viral delivery has several advantages over other mechanisms. For one, many viral platforms...

...Best for: very large deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s...primary difference from Cas9 is that it generates staggered breaks, also known as sticky ends. Cas12a cleavage...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...modern version of this older system, with added advantages that will be discussed in detail in a separate...post. Focusing on the ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate...

...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...optogenetics in specific cell populations even easier. Advantages and other possibilities It’s clear that Cre-DOG...GFP-labeled lines, and they each have their own advantages. T-DOGGs can be made drug-inducible using the...

... consultation meeting or call for free.  Take advantage of this to get the information you need to choose... and speaker is Gwen Acton of Vivo Group whose tagline is Bridging Science and Business. Maybe you just...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

...science, dating back several hundred years, with historical ties to psychology and philosophy (the mind-body...generally mediates broad cell tropism and has a high percentage of neuronal targeting after stereotaxic injection...Alternatively, for larger brain areas, repeated staggered injections may be considered. Timing: High expression...

...: Sign up for a Twitter account. Check out the hashtag #scicomm and start following science communication...local pub at a Pint of Science event. Or get on stage for a Science Slam – a sort of competitive TED talk...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

... popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity...applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers ...research is still in the pre-clinical/early clinical stages. Researchers are also working to make lentiviral...

...cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...

...bacteria, the tumor cells that escape the elimination stage acquire genetic alterations and alter cell-surface..., by way of favorably attaining the equilibrium stage. This can be called “stalemate equilibrium” where...the acute immune responses that eliminate early stage cancer cells, chronic inflammatory responses can... downregulate the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces...

...21 base pairs downstream of the TAM, creating staggered double-strand breaks like those created by Cas12a... like Cas9. This is where TnpB offers a clear advantage. At approximately one-third the size of Cas9 or...Plant Biology: New Tools for an Old Favorite A History of Genome Editing in Popular Culture Additional...

...have scientists solved this problem? By taking advantage of repair pathways within the cell — in some cases...Created with BioRender.com. Base editors take advantage of this single-stranded DNA (ssDNA) region, using...the SOX2 activation domain (SAD), which recruits histone acetyltransferase to open chromatin, improved editing... aspiring genetic editors can now compare the advantages of base editing and prime editing, an alternative...

...HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription... treatment with CRISPR/Cas9 SAM increased the percentage of cells with activated HIV-1, measured via HIV... be more successful. “Shock and kill” has the advantage of killing cells infected with HIV-1 to deplete...

... Tabebordbar et al. Long et al. show that the percentage of dystrophin-expressing muscle cells increases... blot, at <10% of the wild-type level. Muscle histology is improved, with vastly reduced inflammation ...delivery must be optimized to: 1. Reach a high percentage of muscle cells throughout the body, especially...

...with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to...fluorophores to visualize multiple targets at once. An advantage of multiplexing is that you can ask how multiple...choose can make or break your experiment, so take advantage of all the available resources and give yourself...

... of the gel. For small proteins, use a higher percentage of acrylamide to increase migration resistance...improve separation. For large proteins, reduce the percentage of acrylamide. To further improve resolution,...and references References Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory...