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We narrowed to 29 results for: his-tag

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Showing: 1 - 20 of 29 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...with N-terminal Myc tag and attB for Drosophila transgenesis His Epitope tag pEZYmyc-His - C-terminal ...fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein Common uses...bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal 3x HA tag fusion with your gene...bacteria with N-terminal His-TrxA-TEV tag NusA Protein purification pNIC-HIS6-NUSA-TEV-GG - Cloning of...collection page Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools...stop codon for C-terminal tags and omit the start codon for N-terminal tags. And when you are designing... 2xFLAG tag in pCS for a variety of systems, including Xenopus HA Epitope tag pcDNA3.1...

  2. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... (CAPTURE.) Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent...include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed... not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus... EM, Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . ... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...

  3. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression pET51b-His-TEV-HaloTag7 - Bacterial Expression SNAP-Tag Benzylguanine (BG) pSNAP-tag (m) Vector -...Expression pSNAP-tag (T7) Vector - Bacterial Expression CLIP-Tag Benzylcytosine (BC) pCLIP-tag (m) Vector -...Expression pBAD/HisB-Slow-FT - Bacterial Expression Return to top Self-Labeling Tags These tags have been engineered...self-labeling tags on our blog . Protein Ligand Plasmids HaloTag Chloralkane (CA) pMH-Halo tag - Mammalian...CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag - Bacterial Expression Return to top Additional...empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest...Photoconvertible Photoswitchable Timers Self-Labeling Tags Additional Resources The following tables list many...

  4. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...map, a TEV cleavage site is present between the 6xHis-tag and the coding region of MDH. For detailed clone...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Trono 14666 pQE9-His-p97(wt) VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren...17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS Hemmo...0005 VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS...0016 VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS...0009 VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS...0008 VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS...0020 VCP His T7 ALS Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS...

  6. Institute for Protein Innovation

    Type
    Collection
    ...Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags and Other Markers Antibody...Cleft Antibodies NEW Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids (Link opens in a new... (Neurexins and Neuroligins), and common epitope tags, as well as antibody plasmids to create or express...Recommended Applications Return to top Browse Epitope Tag Antibodies IPI has developed recombinant versions...versions of antibodies that recognize common epitope tags to help purify, detect, or localize particular proteins...s full catalog of antibodies that target epitope tags and other common cellular proteins. ID Recombinant...high-confidence targeting of proteins that have historically been difficult to identify and interrogate with...

  7. Bacterial Expression Systems

    Type
    Collection
    .... Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep II...bacteria with different purification tag combinations, including PelB tags for localization to the periplasm...Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization To determine...Fluorescent Protein Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor) YPet (acceptor) FRET Patrick Daugherty..., proteins are often expressed alongside epitope tags, which can also later be used for protein detection...removed using protease cleavage sites. Other protein tags and signal peptides are used to enhance protein ...II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, factor...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ...DNMT1 with a Myc tag. (Li et al. J Biol Chem. 2006) Express human ATM with Flag and His tags. (Canman et al.... Express EGFP-tagged wt and mt tau . (Hoover et al. Neuron. 2010) Express EGFP-tagged wt and mt Alpha-synuclein...myelination. (Lam et al. Nat Commun. 2022) Endogenously tag gene variants for Alzheimer's disease and related...subcellular localization studies with GFP, or IP with Flag tag. (Bennett et al. Heliyon. 2020) Use VAP expression...Chemogenetics Plasmids . Find plasmids for endogenous gene tagging in human cells from the Allen Institute for Cell...are sporadic, having no prior family history. A small percentage (5–10%) are familial ALS cases having...

  9. Biosensor AAV Preps

    Type
    Collection
    ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Eric Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Eric Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...-SV40 Syn CaMPARI2-L398T his Constitutive 1 Eric Schreiter Calcium Sensor: jRGECO1 100852 pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40...Sensors GRAB_NE nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ASAP ATP Sensors...pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Mark Histed 176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none...GRAB_OTmut (control) none Constitutive 1, 9 Yulong Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2...Syn Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...

  10. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2... Schreiter AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 Biosensor Eric Schreiter...James M. Wilson AV-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Control Joshua Sanes AV-9-PV2454 45186-AAV9 AAV-EF1a-BbChT...

  11. Genetic Code Expansion

    Type
    Collection
    ...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta...DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta ...Alfonta 160089 pPaGE Pyl TAG FliC T248TAG PylRS M. mazei Bacterial TAG Lital Alfonta 160377 pDule-Mb haloTyrRS...TAA Abhishek Chatterjee 228784 pAcBac1-Pyl-TAG PylRS M. barkeri Nε-boc-lysine (BocK) Mammalian TAG Abhishek...228785 pAcBac3-EcLeu TAG-EGFP** LeuRS E. coli Cy5-azide (Cy5Az) Mammalian TAG Abhishek Chatterjee 228786 ...coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG Jesse Rinehart 71403...Cyanobacterial TAG Lital Alfonta 99222 pTECH-chPylRS(IPYE) PylRS Chimeric Nε-boc-lysine (BocK) Bacterial TAG Dieter...

  12. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106 pNH-TrxT GU269914 Hexahistidine tag, thioredoxin...EF456736 Hexahistidine tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV...pNIC-CH EF199843 Hexahistidine tag, AmpR 26103 pNIC28-Bsa4 EF198106 Hexahistidine tag with TEV cleavage...GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR 26108 pFB-LIC-Bse EF199842 Hexahistidine tag with... EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag and hexahistidine...hexahistidine tag with Thrombin cleavage, KanR 26096 pET28-MHL EF456735 Hexahistidine tag with TEV cleavage...pCW-LIC EF460848 No tag, double ptac promoter, AmpR 26099 pFBOH-LIC EF456740 Hexahistidine tag with Thrombin...

  13. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells...with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in human cell...You May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization...collection (Link opens in a new window) of fluorescently-tagged human induced pluripotent stem cell (hiPSC) lines...researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell...TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) 114413 MYL7-mEGFP AICSDP-46 mEGFP MLC-2a Sarcomeric...

  14. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Plasmid #68307) ) at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein...Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP...68305 Beta lactamase S68TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68301 MBP-MEK1 S218TAG Plasmid 68300 MBP-MEK1... GFP S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ ...Plasmid 68283 SepOTSβ Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO MBP-MEK1 XX Amp) Strain...Pooled Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid 68307 SupD Strain 68306...BL21ΔserB Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 34623 SepOTSα SepRS/EF-Sep ( aka...

  15. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...can be found in Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of...plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins...Collection Botman-Teusink Yeast FP Collection FP Tagging FP Overexpression Resources This comprehensive ...allows for constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902...). Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-...

  16. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ... mammalian) Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5)...pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Addgene Resources Blog: Synthesized...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ...of a TALE, along with strong nuclear localisation tags and repress transcription initiation when targeted... plasmids 47388 and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus, a 152-residue ...the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64 domain (four... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent... was recently shown that TALE fusions with the histone demethylase LSD1 (Lysine specific demethylase) ...with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation at the target...

  18. All Antibodies

    Type
    Collection
    ...collections: Tags and Other Markers : Antibodies targeting popular markers like tubulin or epitope tags like ...species. We currently assess western blot, immunohistochemistry, and immunocytochemistry. Addgene will continue...

  19. CRISPR Guide

    Type
    Collection
    ...DNA. The system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The...in mammalian cells include: SunTag system — co-expression of epitope-tagged dCas9 and antibody-activator...acid, turn a promoter on/off, or add small protein tags. Precise Modifications Using Homology Directed Repair...Plasmids: Double-Strand Break (Cut) , Endogenous Tagging CRISPR Base Editing To overcome low HDR efficiency...also be fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR ...dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing...sequence specified by a particular gRNA. Epitope tag(s) are fused to dCas9 or gRNA for efficient purification...

  20. Antibody Guide

    Type
    Collection
    ...the sdAb, or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have ...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...methods such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use... they only have one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can... can refer to both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (...
Showing: 1 - 20 of 29 results