Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 266 results for: his-tag

Showing: 1 - 20 of 266 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium Bicarbonate... 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots of FBS can... sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, we have found ...sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2% HI-FBS into a sterile 1 L bottle. Add each plasmid DNA...solution to the bottle containing 774 mL of DMEM + 2% HI-FBS. Mix well. Take the CS5 out of the incubator...L-alanyl-L-glutamine (or alternative stable glutamine such as glutaGRO, Corning 25-015-CI) Opti-MEM, Thermo Fisher 31985...L-alanyl-L-glutamine (or stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add ...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...with N-terminal Myc tag and attB for Drosophila transgenesis His Epitope tag pEZYmyc-His - C-terminal ...fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein Common uses...bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal 3x HA tag fusion with your gene...bacteria with N-terminal His-TrxA-TEV tag NusA Protein purification pNIC-HIS6-NUSA-TEV-GG - Cloning of...collection page Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools...stop codon for C-terminal tags and omit the start codon for N-terminal tags. And when you are designing... 2xFLAG tag in pCS for a variety of systems, including Xenopus HA Epitope tag pcDNA3.1...

  3. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized... suitable for creating individual fluorescently tagged proteins to study protein-protein interactions ...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Trono 14666 pQE9-His-p97(wt) VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren...17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS Hemmo...0005 VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS...0016 VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS...0009 VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS...0008 VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS...0020 VCP His T7 ALS Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... (CAPTURE.) Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent...include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed... not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus... EM, Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . ... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...

  6. To Each HIS Own

    Type
    Blog Post
    ...purification based on the polyhistidine tag. What is the polyhistidine tag? The polyhistidine tag is a string of ...Benefits of HIS tags In addition to providing a way to purify a protein of interest, polyhisitidine tags can ...low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended if... isolated.   Prior to the polyhistidine tag, alternative fusion tags were used, such as S. aureus’... conditions.   Find polyhistidine tag plasmids here! Where should I place the tag and how many do I need...plasmid containing a polyhistidine tag, you’ll need to decide whether to add the tag to the N- or C-terminus... however, tag placement can affect protein function. B, The string of histidines in the tag is typically...

  7. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...map, a TEV cleavage site is present between the 6xHis-tag and the coding region of MDH. For detailed clone...

  8. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...covered! His His tags, often called polyhistidine tags, typically include six consecutive His residues...Diagram of His-tagged protein purification work flow by nickel column.    Affinity tags vs. other ...other tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent...blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each HIS Own ...planning, affinity tags can make protein purification a cinch. Types of affinity tags, the proteins they...are all reviewed here. What are affinity tags? Affinity tags are used primarily as protein purification... common tags available. Maltose binding protein Maltose binding protein (MBP) is a 45 kDa tag which can...

  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene...empty plasmid backbones with different fluorescent tags for you to create fusion proteins with your gene...Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian Expression...dimerization pCEP4YPet-MAMM - Mammalian Expression pYPet-His - Bacterial Expression YPet-N1 - Mammalian Expression...Monomer pmiRFP670-N1 - Mammalian Expression pBAD/His-miRFP670 - Bacterial Expression iRFP670 643 670 13...Monomer pmiRFP703-N1 - Mammalian Expression pBAD/His-miRFP703 - Bacterial Expression iRFP702 673 702 8...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    ...affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function, His-tagged proteins...usually using a Ni2+ column. Like other affinity tags, a His tag can be fused to either the N- or C-terminus...Combo and cleavage tags Frequently, a single tag is not enough. What if you need one tag to increase solubility... to a purification scheme, His tags are frequently combined with other tags for dual-labeling. The problem...to all tags, but rather a quick overview of why scientists use tags, with a few time-tested tags and fusion...length a polyhistidine tract does not greatly alter the size of the tag.   Table 1: Common protein tags ...keep your protein soluble.  Tags for affinity and purification An affinity tag, generally a relatively small...

  11. Institute for Protein Innovation

    Type
    Collection
    ...Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags and Other Markers Antibody... Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids Welcome to the Institute...proteins (glypicans and integrins) and common epitope tags, as well as antibody plasmids to create or express...Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed recombinant versions...versions of antibodies that recognize common epitope tags to help purify, detect, or localize particular proteins...s full catalog of antibodies that target epitope tags and other common cellular proteins. ID Recombinant...

  12. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer that has now prompted...State University has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive... all those collaborations over the years, he and his colleagues built a Molecular Expressions collection...spectra. A comprehensive list of cell markers all tagged with mEmerald. Plasmids to monitor protein-protein...available targets since the Davidson lab always tagged new proteins with mEmerald first, Baird says. From...cell membrane, this collection has fluorescently tagged versions of many proteins known to localize to ...prompted him to deposit the tools he and his team created with the research community at large, "[Fluorescent...

  13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have a FRET acceptor-binding...for protein purification: the NTA binds to the His tag on your protein and the coupled Cy fluorophore ...C-terminal fusion of a tetracysteine tag. In addition, since these tags are so small, one can insert them... between 80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby...interest: you just don’t know it yet! The poly-histidine tag often used for protein purification is a reliable..., as well as by extending the length of the histidine tag from 6 to 10 residues. While these Cy/NTA conjugates...incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni2+: ...

  14. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...same kinds of opportunities for his students. But he also found that his teaching responsibilities left...limited time for one-on-one mentoring. His solution? Bringing his research into the classroom. I had the...to hear about how he developed his program. Initially, Provost made his labs completely open-ended, allowing...bacteria, purified them to near homogeneity using a 6XHis tag, and compared their specific activities by measuring...purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent...San Diego), decided to take a different approach. His own most impactful learning experiences happened ... opportunity to talk with Provost recently about his development of what is now commonly termed a Course-based...

  15. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...Campus in Aurora, Colorado, USA. The primary focus of his lab’s research is the mechanisms by which proteins...Gronostajski is a professor at the University of Buffalo. His research focuses on understanding the structural ...research on diseases of glycosylation, arginine mutagenesis, and neural development. Paula Videira: Congenital...as reporters of NFIX function. They will then mutagenize the NFIX gene and test whether variants of unknown...

  16. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...

  17. Biosensor AAV Preps

    Type
    Collection
    ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...L398T-WPRE-SV40 Syn CaMPARI2-L398T his Constitutive 1 Schreiter Calcium Sensor: jRGECO1 100852 pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40...Sensors GRAB_NE nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ASAP ATP Sensors...-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Histed 176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2...KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...

  18. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...promoters, N-terminal tags, coding sequences of the gene of interest, C-terminal tags, plant terminators ...science lab.” Introducing GreenGate As Lohmann and his colleagues describe in the PLoS ONE paper that introduced... it no longer works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and...aptly named GreenGate – offers all of those same advantages to researchers working in plant model systems...

  19. Bacterial Expression Systems

    Type
    Collection
    .... Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep II...bacteria with different purification tag combinations, including PelB tags for localization to the periplasm...Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization To determine...Fluorescent Protein Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor) YPet (acceptor) FRET Patrick Daugherty..., proteins are often expressed alongside epitope tags, which can also later be used for protein detection...removed using protease cleavage sites. Other protein tags and signal peptides are used to enhance protein ...II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, factor...

  20. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    ..., too! Bluegene is eager to decorate the lab but his short arms are causing him some difficulty… To give...celebrating the holidays and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly...you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of December...
Showing: 1 - 20 of 266 results