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We narrowed to 416 results for: iCre

Showing: 221 - 240 of 416 results
  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128/AEM.02296...cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007...
  2. A Control for All Seasons

    Type
    Blog Post
    ...protocol remains the same.    Figure 2: Microscopy-based antibody experiments should include a primary...Accuracy and Precision in Quantitative Fluorescence Microscopy.” Journal of Cell Biology, vol. 185, no. 7, Rockefeller...
  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...plasmid components are brought together and microinjected into either the pronucleus or the cytoplasm ...reagents. Other than that, be nice to your microinjectionist, who has the strategically important job of...
  4. Finding Your Perfect Job After University

    Type
    Blog Post
    ...field require experience. Working in industry: Microbiology On returning from Ghana at the beginning of ...Industry do tend to pay better. I worked in a Microbiology testing facility, where I tested materials for...
  5. Plan Your Experiment

    Type
    Collection
    ...which you can deliver to target cells through microinjection or electroporation. The target cells translate...
  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...ask incredulously, “You seriously didn’t add 3 microliters of 5 mM star anise?” This is something I was ... could see GFP expression with a fluorescence microscope, but the GFP expression was so low (even in the...
  7. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...relatively small amount of DNA (in the nanograms to micrograms scale depending on the experiment), but issues...require quite large amounts of DNA (easily in the micrograms scale and up). But perhaps more importantly, ...
  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...expression. Check out Sano et al to learn how microRNAs can be used to regulate Sendai virus transgene...Using a Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed PMID: 27764162...
  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Light Inducible Dimers (iLID), iLID nano and iLID micro, which differ from each other by their light/dark... 4.7µM for iLID nano and 800nM to 47µM for iLID micro; Figure 1). With this great affinity range, these...BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin Early Endosome ...
  10. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...available through Addgene. Stovicek, et al. J Ind Microbiol Biotechnol. 2015. PubMed PMID: 26376869 Jensen...one of them called “claudin-like apicomplexan microneme protein” (CLAMP) was shown to be important for...
  11. Editor's Choice, July 2016

    Type
    Blog Post
    ...excellent posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR...
Showing: 221 - 240 of 416 results