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TypeBlog Post...of the most important steps in the design of an imaging experiment. The choice is non-trivial, however...Choosing Your Fluorescent Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent...
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Visualizing Translation at the Single Molecule Level
TypeBlog Post...location of the ribosome, TRICK (Translating RNA Imaging by Coat protein Knock-off) instead takes advantage... M., et al. “Translation. An RNA biosensor for imaging the first round of translation from single cells... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...you have done fluorescent imaging, then you probably have experience using image analysis software. If you... -
Antibodies 101: ChIP
TypeBlog Post... Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons. Okay...actually celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/ Even... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post... hybridomas for monoclonal antibody production. Image created with BioRender.com. The antibody-producing...different antibodies produced by the hybridoma. Image from Bradbury et al., 2018. Another barrier... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post... Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve introduced...fluorescence. Figure 2: The GEmCherry reporter. Image from Højland Knudsen et al., 2018. So how does... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...30704911. Acoustic reporter genes for noninvasive imaging of microbes in mammalian hosts Article contributed...microbiome systems that are potentially problematic for imaging. Find the ARG plasmids at Addgene. For more details... -
Viral Vectors 101: Biosensors
TypeBlog Post...are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors can be...of them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F,...small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb... -
Antibodies 101: Affinity Reagents
TypeBlog Post... space that would size-exclude an antibody. In imaging applications, smaller affinity reagents means higher...often used for clinical applications, including imaging. Diabodies are ~60 kDa proteins formed by two ... -
All in a Twist: dsRNA
TypeBlog Post... molecules are clearly associated with cellular damage and dysfunction, it is unclear what the precise... that is now vulnerable to various forms of DNA damage, and these structures can in turn lead to genome... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...regulatory networks within E. coli host cells. Image from Nielson and Voigt (2014). We talked with...because they are used to create any program you can imagine. The Apollo 11 mission circuitry that took a spaceship... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...succumb to the toxicity of CcdB. Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 ...desired insert should not grow. Figure 2: Image from http://teachline.ls.huji.ac.il/72682/tutorials... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...An assembled antibody protein binds an antigen. Image from Fvasconcellos. How are Recombinant ...stability, and design tools that we haven’t even imagined yet. References and Resources References Andrews... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post... site. TFBS: transcription factor binding site. Image from Jüttner et al., 2019 with permission. In... humans (R=0.67). A database of the microscopy images of all AAVs that reproducibly labeled retinal cells... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post... fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep 20;111(6):1119-31...2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically encoded calcium... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...offer guided tours to show people where the lab magic is happening. 3) Volunteer – Step into science education...learning on the job. Try to get an internship at a magazine or newspaper (here’s a partial list). A great ... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...Cell Collection at Coriell. More information and images generated from these cell lines can be found in...fluorescent proteins as described here enables live cell imaging to study cellular structures and processes and ... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...fluorescent signaling in the presence of dsDNA. Image courtesy of: Ygonaar Taqman is more sensitive...thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique, courtesy ...