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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 474 results for: mag

Showing: 221 - 240 of 474 results

  1. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...sample were loaded in wells B01-H01. As shown in the image (Figure 1) and table below, the concentration of... to the lower dilutions (blue). As shown in the image (Figure 2) below, the concentration of RPP30 positive...

  3. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors can be...of them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in...

  4. Antibodies 101: ChIP

    Type
    Blog Post
    ...    Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay...actually celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even...

  5. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing...different antibodies produced by the hybridoma. Image from Bradbury et al., 2018.   Another barrier...

  6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ... Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve introduced...fluorescence.  Figure 2: The GEmCherry reporter. Image from Højland Knudsen et al., 2018. So how does...

  8. All in a Twist: dsRNA

    Type
    Blog Post
    ... molecules are clearly associated with cellular damage and dysfunction, it is unclear what the precise... that is now vulnerable to various forms of DNA damage, and these structures can in turn lead to genome...

  9. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ... space that would size-exclude an antibody. In imaging applications, smaller affinity reagents means higher...often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two ...

  10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...succumb to the toxicity of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 ...desired insert should not grow.   Figure 2: Image from http://teachline.ls.huji.ac.il/72682/tutorials...

  12. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep 20;111(6):1119-31...2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically encoded calcium...

  13. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ...

  17. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Cell Collection at Coriell. More information and images generated from these cell lines can be found in...fluorescent proteins as described here enables live cell imaging to study cellular structures and processes and ...

  18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ... Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   ...recipient bacteria with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    ...

  19. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...fluorescent signaling in the presence of dsDNA. Image courtesy of: Ygonaar    Taqman is more sensitive...thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique, courtesy ...

  20. Transferable Skills: Negotiation

    Type
    Blog Post
    ...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for...manuscript is more important to you than collecting your images this week. Even though three examples depend on...
Showing: 221 - 240 of 474 results