We narrowed to 565 results for: mme

...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...use in your experiment! As a starting point, we recommend checking the literature and our AAV data hub to...

...increase capture. Longer histidine tags are also recommended if you need a very pure sample. Because binding...antibody, like this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would...

...from — Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve...introductions to lots of molecular biology,” — Reddit commentor Yay4sean, in r/molecularbiology.   Oh, ...

...consider leaving a link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M....supernatant is only $40, significantly cheaper than commercially available antibodies. ...

...student. For post-grad school individuals, Susan recommends starting a blog, but with one caveat: “I hate...Association of Biomedical Research. She does, however, recommend using Twitter as a way to leverage unpaid work...

...resistant to the toxic effects of CcdB. Another commercially available, CcdB-resistant strain is ccdB Survival...these resources further. Feel free to offer your comments and suggestions, and even to submit a guest blog...

...Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

... with our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told me, “I think...favorite response to this question came from Lucy Hammer, our Financial Operations Specialist. She said,...

...are always required and lab coats are either recommended or required in BSL-1 and required in BSL-2 labs...with lentivirus, many organizations require or recommend disposable gowns over your lab coats and double...

...antibody. Manning et al. tested a variety of commercially available secondary antibodies and found that...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

...just right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction...component plasmid, component name, size, and any comments. You can use the ID to look up the component plasmids...

...regeneration-related genes in four inexpensive and commercially available planarians: Girardia sp., Dugesia ...IPTG-induced expression to work, students have to use a commercial E. coli strain that is designated as DE3. For...

...past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ ...not; applications for funding from charities, commercial organizations, or universities may have someone...

...that this has precipitated increases to the NIH recommended pay scale – used by many institutions to set ...PMC4304227. 4. Revitalizing biomedical research: recommendations from the Future of Research Chicago Symposium...

...come to a meeting with a clear agenda, you will immediately get your supervisor’s attention so you can focus...supervisors might try to intimidate you with negative comments (hostile-aggressives, complainers, know-it all...

...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...separately in a future post.   Resources Maria recommends: Getting to Yes: Negotiating Agreement Without...

... Highly expressed markers should be paired with dimmer dyes so they don’t “wash out” the rest of the panel...anything (negative population). It is strongly recommended that when you first run a new panel on a spectral...

... liquid containers closed when they aren’t in immediate use. This will prevent spills, but, if any spills...occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution...

...poured onto the middle of the plate, where it immediately spreads out to form a firm monolayer. The cell...the level of endogenous expression. We highly recommend the use of an electronic pipettor with variable...

...Addgene Blog - Leave suggestions and tips in the comments section below or contact [email protected] if you'd...scientific community on NgAgo forums, blogs, or in the comments section below. Keep pipetting and happy genome...