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We narrowed to 565 results for: mme

Showing: 221 - 240 of 565 results

  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...consider leaving a link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M....supernatant is only $40, significantly cheaper than commercially available antibodies. ...

  3. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...use in your experiment! As a starting point, we recommend checking the literature and our AAV data hub to...

  4. To Each HIS Own

    Type
    Blog Post
    ...increase capture. Longer histidine tags are also recommended if you need a very pure sample. Because binding...antibody, like this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would...

  5. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...These modifications can be done in-lab using commercially available kits or ordered as synthesis modifications... to do-it-yourself with a kit or purchase it commercially synthesized with a label. Not enough options...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...their bulk can be a barrier at times. Smaller, slimmer affinity reagents can have deeper penetration into...Single-domain antibodies (sdAbs), sold under the commercial name nanobodies, are derived from a camelid antibody...

  7. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...resistant to the toxic effects of CcdB. Another commercially available, CcdB-resistant strain is ccdB Survival...these resources further. Feel free to offer your comments and suggestions, and even to submit a guest blog...

  8. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...just right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction...component plasmid, component name, size, and any comments. You can use the ID to look up the component plasmids...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...are always required and lab coats are either recommended or required in BSL-1 and required in BSL-2 labs...with lentivirus, many organizations require or recommend disposable gowns over your lab coats and double...

  11. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...antibody. Manning et al. tested a variety of commercially available secondary antibodies and found that...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

  14. 6 Tips for Grant Writing

    Type
    Blog Post
    ...past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ ...not; applications for funding from charities, commercial organizations, or universities may have someone...

  18. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... liquid containers closed when they aren’t in immediate use. This will prevent spills, but, if any spills...occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution...

  19. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ... Highly expressed markers should be paired with dimmer dyes so they don’t “wash out” the rest of the panel...anything (negative population). It is strongly recommended that when you first run a new panel on a spectral...

  20. PhD Applications After COVID

    Type
    Blog Post
    ...during the application process. Many institutions recommend that you reach out to potential advisors before...professors per program. Most applications will recommend that you name two professors in your application...
Showing: 221 - 240 of 565 results