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We narrowed to 560 results for: mme

Showing: 221 - 240 of 560 results

  1. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...their bulk can be a barrier at times. Smaller, slimmer affinity reagents can have deeper penetration into...Single-domain antibodies (sdAbs), sold under the commercial name nanobodies, are derived from a camelid antibody...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...use in your experiment! As a starting point, we recommend checking the literature and our AAV data hub to...

  3. Addgene: The First Twenty Years

    Type
    Blog Post
    ...from — Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve...introductions to lots of molecular biology,” — Reddit commentor Yay4sean, in r/molecularbiology.   Oh, ...

  4. To Each HIS Own

    Type
    Blog Post
    ...increase capture. Longer histidine tags are also recommended if you need a very pure sample. Because binding...antibody, like this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would...

  5. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...These modifications can be done in-lab using commercially available kits or ordered as synthesis modifications... to do-it-yourself with a kit or purchase it commercially synthesized with a label. Not enough options...

  8. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...are always required and lab coats are either recommended or required in BSL-1 and required in BSL-2 labs...with lentivirus, many organizations require or recommend disposable gowns over your lab coats and double...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...antibody. Manning et al. tested a variety of commercially available secondary antibodies and found that...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

  10. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...just right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction...component plasmid, component name, size, and any comments. You can use the ID to look up the component plasmids...

  12. 6 Tips for Grant Writing

    Type
    Blog Post
    ...past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ ...not; applications for funding from charities, commercial organizations, or universities may have someone...

  16. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... liquid containers closed when they aren’t in immediate use. This will prevent spills, but, if any spills...occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution...

  17. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...poured onto the middle of the plate, where it immediately spreads out to form a firm monolayer. The cell...the level of endogenous expression. We highly recommend the use of an electronic pipettor with variable...

  18. Transferable Skills: Negotiation

    Type
    Blog Post
    ...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...separately in a future post.   Resources Maria recommends: Getting to Yes: Negotiating Agreement Without...

  19. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ... Highly expressed markers should be paired with dimmer dyes so they don’t “wash out” the rest of the panel...anything (negative population). It is strongly recommended that when you first run a new panel on a spectral...

  20. PhD Applications After COVID

    Type
    Blog Post
    ...during the application process. Many institutions recommend that you reach out to potential advisors before...professors per program. Most applications will recommend that you name two professors in your application...
Showing: 221 - 240 of 560 results