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We narrowed to 760 results for: mpo

Showing: 221 - 240 of 760 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...Genetically modified organisms like transgenic mice are important tools for understanding the function of cell types...CRISPR edits and improves editing efficiency. Importantly, the point mutations created by Agudelo et al...length with TZAP Maintaining telomere length is important for cellular function and genomic stability, as...multiplex gRNA expression. Expression of toolkit components can be controlled by a variety of promoters for... for versatile and robust engineering. These components are provided in Golden Gate compatible backbones...

  2. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...External collaborations are becoming increasingly important as more complex approaches to scientific questions...collaborations and partnerships are also very important in a non-research context, especially when considering...start and maintain external collaborations is important? In my current job at Addgene I work with people...work with others outside your organization is important for success in research-intensive careers. In ...

  3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...great for studying parenting strategies and make important ecological indicators since species live in water...Aiptasia - algae Breviolum minutum symbionts are important models for solutions to the coral reef crisis....chemical pathways generating interesting drug-like compounds especially cardenolides which have been used to... interesting biologically. They have economic importance as harmful weeds. The parasitic fusion is such...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    ...interchangeable set of fusogenic and targeting components to reprogram delivery vehicles like lentiviral...DIRECTED particles where the fusion and targeting components are interchangeable. Image from Strebinger et...., 2023, reused under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on...in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells...

  5. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...making the materials available to many countries, import and export regulations, differing customs processes...In China, direct shipments weren’t able to clear import control. Partnering with a distributor allowed ...Addgene YouTube channel to add a strong visual component to our educational resources. From 2013–2023, ...expanding our website, we’ve learned about the importance of smaller, systemic changes, like using alt-...

  6. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...molecules that may bind non-specifically to assay components and increase background staining. Direct ELISAs...with binding, and some common antibody buffer components can interfere with conjugates. Make sure to do...adds an antigen-specific detection antibody.  Importantly, the detection antibody and the capture antibody... antibody binding.  We hope that you now feel empowered to choose the best ELISA for your needs. Good ...

  7. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...initiate replication. But pSC101 requires a third component: RepA, a protein encoded by pSC101 itself. RepA... are efficient for the host, as no additional components are necessary. Iterons are not the only tool ...compensating for the additional metabolic cost imposed on the host with a beneficial function (usually... DNA purification. On the other hand, they also impose a lower metabolic cost on cells and produce lower...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed...These differences in recombinase efficiency are important to keep in mind when deciding whether to use the... only occur at a specific time point? To allow temporal control, ligand regulated Cre and FLPe recombinase...instance) can be controlled with FLP-FRT. For further temporal control, Tamoxifen inducible Cre versions can ...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...that generates the chromophore (Tsien, 1998). Importantly, this reaction requires molecular oxygen and ...genetically encoded tag and exogenously added components. For a review see Lotze et al. (2016). To date...is used to excite GFP, may also excite several components that are naturally present in cells or media....limitations. Being aware of the limitations is important for the successful use of GFP or any of the other...

  10. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...directing. Everyone’s schedules were busy, so it was important for me to let people record at their leisure. ...things like lighting, camera angle, and shot composition.  So back to the drawing board.  Cycle 3: A Return... were still many restrictions in place, most importantly, masks.  Besides the muffled voices beneath masks...out of the way when it was blocking some more important lab work from being done. You can watch the first...

  11. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...BioRender.com. Loading controls are even more important for semi-quantitative western blots. No pipetter...independent of these other sources of error. It’s important to validate these assumptions for your particular... 2013). To address this source of error, it’s important to determine the linear detection range of all...indicates higher sensitivity, linearity is the most important feature of a loading control, and both total protein...

  12. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...populations were also immune to HIV-1 reinfection. Importantly, no off-target effects were detected. Kaminski...patients. Although this is an exciting finding, it’s important to note that these HIV-1 patients were ART-naive...Cas9. While these results are discouraging, it’s important to note that these experiments were done in cell...propagate and infect adjacent cells. It will be important to determine if CRISPR/Cas9 escape occurs in an...

  13. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...the 1980s, is one of the most important ways to spatially and temporally control gene expression, especially... 8 colors. The use of AAV provides spatial and temporal control without the need for germline modification...refinements of the Brainbow technique will provide important insights into the complicated physical organization...

  14. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...methylase mutation (GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes... mutation (CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes...propagating plasmids expressing the ccdB gene (important in Gateway cloning). F-mcrA Δ(mrr-hsdRMS-mcrBC...propagating plasmids expressing the ccdB gene (important in Gateway cloning). F- gyrA462 endA1 glnV44 Δ...

  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...individual types of GPCRs with greater spatial and temporal control than is achieved by traditional pharmacologic...pathway. Photoswitches are great for teasing out important branch points along a signaling cascade that may... activated. Created with BioRender.com.   Important considerations for using optogenetic tools Once...can wreak havoc on your experiments, so it is important to understand the details of how each tool performs...

  16. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...signal to gradually diminish over time; and, importantly, repeated excitation generating reactive oxygen...internalized. Functional assays further showed that imported ADAR1 increased oncogenic editing of its most ...which was slowed by knockdown of UBE3C or other components of the degradation pathway. HiBiT enabled the...models, tools that increase confidence become more important. For many difficult-to-study protein targets, ...

  17. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... genome, the CRISPR/Cas system makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies...gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected into either...eggs. Or, to avoid handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant... microinjectionist, who has the strategically important job of delivering the payload! Last but not least...

  18. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest go until completion. The time ...running a gel for purification purposes it is important to have nice crisp bands and to have space to ... your favorite gel purification method, it is important to determine the concentration of recovered DNA...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    ...scientists with an MSc or higher apply. It was important to weigh the cost to benefit of studying a Masters.... I felt then, and still feel now, that it is important to take advantage of the fact that science is ...manufacturing practice (GMP) and good lab practice (GLP) (important buzzwords in industry) as well as skills in industry...permanent position in academia. It is therefore important for young scientists and PhD students to also ...

  20. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...synthesized by eukaryotic cells. First, the kit components rupture the mycoplasma and then specific enzymes...mycoplasma contamination The best and the most important ways to avoid mycoplasma are by providing proper...viability. Conclusion To sum up, it is always important to prevent contamination rather than eradicating...others when you can and help them understand the importance of preventing contamination. Be aware and cautious...
Showing: 221 - 240 of 760 results