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We narrowed to 567 results for: multi

Showing: 221 - 240 of 567 results

  1. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...deletions, mutations, in-frame protein fusions, and multiple cloning sites. In the simplest case we have a ...insert are correct. In other cases, we deal with multiple inserts or internal mutations that require more... DNA-editing software to perform alignments of multiple sequences to visually check for homology and mismatches...

  2. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...barcodes infect the target cell population at low multiplicity of infection (MOI) such that each individual...erlotinib. Looking at barcode abundances among multiple replicate populations, they found that similar...went on to show that combination treatment with multiple drugs reveals even smaller preexisting populations...

  3. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...ELISA is best used to detect proteins for which multiple antibodies that recognize different epitopes are...sensitive to the target protein due to the use of multiple antibodies. Figure 1: Overview of using...primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can bind to a single primary...

  4. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... of time that you want to probe your blot with multiple antibodies. You’ll want to set up your blot with...stripping methods. If you are planning to detect multiple proteins through several rounds of stripping and...clearing the way for the second blot. There are multiple ways to strip blots, and the best choice will ...

  5. Hot Plasmids February 2024

    Type
    Blog Post
    ...achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ...widely successful in primates. The authors used a multispecies approach to generate and screen a library of... Multiple Authors...

  6. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...ways to deal with this issue. One way is to grow multiple bacterial cultures of your AAV transfer plasmid...aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws of your larger aliquots of virus.... a few different concentrations of virus, or multiplicity of infections (MOIs), to determine the optimal...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Clustal is our frequent choice for pairwise or multiple sequence alignments of nucleotide or protein sequences... sequences. We also use COBALT for aligning multiple protein sequences, particularly for comparing different...//www.ebi.ac.uk/Tools/webservices/#multiple_sequence_alignment_msa http://www.ebi.ac.uk/Tools/webservices...

  8. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...exponential decay process we observed, while 𝜓z(t) multiplies the likelihood by the corresponding factor 𝜙...parameter. We approximated the data likelihood with multivariate Gauss-Hermite quadrature, since the analytical...intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects of weekends...

  9. MXS Chaining

    Type
    Blog Post
    ...powerful imaging technologies, the combination of multiple fluorescent protein sequences to develop diverse...individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules are assembled one ...not suited to generate polycistronic containing multiple identical polyadenylation signals or promoters...

  10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...libraries utilize an engineered Cas9, which contains multiple point mutations in the nuclease domain. These ...affected by gene activation rather than depletion. Multiple strategies have been developed to recruit transcriptional...domain such as VP16 to dCas9 or the recruitment of multiple copies of VP16 termed the “Sun Tag” method (Tanenbaum...

  11. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...individual genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the... then packaged into AAV for in vivo delivery. Multiplex gene targeting is also possible and is only constrained...vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator...

  12. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

  13. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate AAV vectors, increasing overall... AHM, Zhu K, Wagers AJ, Church GM (2016) A multifunctional AAV–CRISPR–Cas9 and its host response. Nat ... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...CRBNmidi alone (A) and in a ternary complex with the multiple myeloma drug mezigdomide and ubiquitination substrate...mutations.  Here, the researchers were able to target multiple genes in M. abscessus either simultaneously or... Multiple Authors...

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    ...different viral families and can be used to target multiple delivery systems, such as lentivirus and MMLV ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi... Multiple Authors...

  16. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...maintain itself within host cells.  The plasmid has multiple ori that are functionally differentiated from ...proteins from bacterial hosts. In a plasmid with multiple oris, one may function in one type of host and...only one ori and a broad host range plasmid with multiple oris that vary in DnaA-box and iteron structure...

  17. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... not, multiple techniques are used as part of a treatment plan, an approach known as multimodal therapy...are some genetic factors involved. For example, multiple studies show that certain mutations of the BAP1...

  18. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...et al., 2019). Because of its ability to target multiple gene loci simultaneously, CRISPRa can also be ...(Chavez et al., 2015)) are good choices across multiple cell lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3...Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods 13:563–567 . https://doi....

  20. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...calculate things about earlier ones. In practice, multiple sources of error (red) influence these relationships...not perturbed by the experiment Unfortunately, multiple reports suggest that common housekeeping proteins... to worry about the linear detection ranges of multiple targets. Convenient! Comparison of loading control...
Showing: 221 - 240 of 567 results