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We narrowed to 575 results for: multi

Showing: 221 - 240 of 575 results

  1. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...Massachusetts Medical School developed a dCas9-based multicolor labeling system to visualize chromosomal loci...plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR labeling of chromosomal loci in human ...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

  2. Hot Plasmids - January 2023

    Type
    Blog Post
    ...only having one binding site per protein. Having multiple antibodies allows you to use them in parallel ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...  Multiple Authors...

  3. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ... enzymes, some of which are also functional in multiple species (read Arbor Biotechnologies' press release...nt direct repeat) mediating potent knockdown. Multiplexing from the CRISPR array yielded >90% knockdown... necessary. Konermann et al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference...

  5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... cell research has prompted the development of multiple virus packaging methods. Across these methods,...sequence. This post was written and updated by multiple Addgenies. It was originally published in 2018...  Multiple Authors...

  6. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...autofluorescence and phototoxicity and even allowing multiple proteins to be visualized simultaneously. Complementation... with incompatible substrates can also allow multiplexing with experiments that supply both substrates... Multiple Authors...

  7. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...deeply connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine...spread and drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes...proliferate and cannot undergo apoptosis, thus multiplying uncontrollably and building up tumors. “Friendly...

  8. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...deletions, mutations, in-frame protein fusions, and multiple cloning sites. In the simplest case we have a ...insert are correct. In other cases, we deal with multiple inserts or internal mutations that require more... DNA-editing software to perform alignments of multiple sequences to visually check for homology and mismatches...

  9. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...barcodes infect the target cell population at low multiplicity of infection (MOI) such that each individual...erlotinib. Looking at barcode abundances among multiple replicate populations, they found that similar...went on to show that combination treatment with multiple drugs reveals even smaller preexisting populations...

  10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...ELISA is best used to detect proteins for which multiple antibodies that recognize different epitopes are...sensitive to the target protein due to the use of multiple antibodies. Figure 1: Overview of using...primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can bind to a single primary...

  11. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... of time that you want to probe your blot with multiple antibodies. You’ll want to set up your blot with...stripping methods. If you are planning to detect multiple proteins through several rounds of stripping and...clearing the way for the second blot. There are multiple ways to strip blots, and the best choice will ...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    ...achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ...widely successful in primates. The authors used a multispecies approach to generate and screen a library of... Multiple Authors...

  13. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...ways to deal with this issue. One way is to grow multiple bacterial cultures of your AAV transfer plasmid...aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws of your larger aliquots of virus.... a few different concentrations of virus, or multiplicity of infections (MOIs), to determine the optimal...

  14. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Clustal is our frequent choice for pairwise or multiple sequence alignments of nucleotide or protein sequences... sequences. We also use COBALT for aligning multiple protein sequences, particularly for comparing different...//www.ebi.ac.uk/Tools/webservices/#multiple_sequence_alignment_msa http://www.ebi.ac.uk/Tools/webservices...

  16. MXS Chaining

    Type
    Blog Post
    ...powerful imaging technologies, the combination of multiple fluorescent protein sequences to develop diverse...individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules are assembled one ...not suited to generate polycistronic containing multiple identical polyadenylation signals or promoters...

  17. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...exponential decay process we observed, while 𝜓z(t) multiplies the likelihood by the corresponding factor 𝜙...parameter. We approximated the data likelihood with multivariate Gauss-Hermite quadrature, since the analytical...intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects of weekends...

  18. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...libraries utilize an engineered Cas9, which contains multiple point mutations in the nuclease domain. These ...affected by gene activation rather than depletion. Multiple strategies have been developed to recruit transcriptional...domain such as VP16 to dCas9 or the recruitment of multiple copies of VP16 termed the “Sun Tag” method (Tanenbaum...

  19. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...when digested with restriction enzymes common to multiple cloning sites, will result in similar sized bands...enzyme or enzymes that will cut your plasmid into multiple fragments, you can get a very unique pattern that...

  20. Video Library

    Type
    Protocol
    ...Technique Learn how to pipette multiple samples at once using a multichannel pipette and avoid inconsistent...
Showing: 221 - 240 of 575 results