We narrowed to 285 results for: nif

1. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making...

2. Of Myc and Men

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   Blog Post

   ... the N-terminal half of the antigen differs significantly between human and the other species, suggesting...

3. Hot Plasmids and Viral Preps - May 2021

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   ...10.1101/2021.01.30.428974   The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   ...Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology of ICEBs1, an integrative ...

5. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   ...-Crick base pair substrates, it demonstrates significant activity on some mismatch-containing pairings...

6. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   Blog Post

   ... Jennifer Tsang...

7. The AAVantages of AAV in CRISPR Screens

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   ...diseases, as you can target your library to a significant degree using different types of AAV. Expression...

8. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   ...Maria’s screens of AsLOV2 variants showed some significant gains! After a few more rounds of mutagenesis...

9. Tips to Make the Most of a Scientific Conference

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   Blog Post

   ... Jennifer Tsang...

10. Viral Vectors 101: Voltage Indicators

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    Blog Post

    ...comparable to its one component counterpart and is significantly brighter and available with red-shifted fluorophores...

11. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...Organism box. This small piece of information can significantly reduce your wait time for blastn, blastp, and...

12. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post

    ...antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially available antibodies...

13. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

    ..., confirming again that sgRNA design plays a significant role in library efficiency. Try the Dolcetto ...

14. DNA Purification Without a Kit

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    Blog Post

    ...have advantages: they are convenient and provide uniform, consistent results. But they are also less accessible...

15. AAVs in Retinal Gene Therapy

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    Blog Post

    ...reported results are encouraging. Importantly, a significant amount of basic research is being done to further...

16. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...wall synthesis step during cell division. This is nifty because the recovery period after transformation...

17. An Introduction to Adenovirus

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    Blog Post

    ...basic research, like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While...

18. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post

    ...(ESCs). Your ChIP-seq finds just over 13,000 significant binding peaks for NANOG in the human genome. ...

19. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    ...polymerase activity, whereas some ratios result in significantly reduced activity (4). To ensure that you end...

20. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...