We narrowed to 363 results for: reporter

1. Live and Let Dye: Self-Labeling Protein Tags

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   Blog Post

   ... self-labeling tools: Tetracysteine: The first reported chemical labeling tag, from Roger Tsien’s lab ...collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts...

2. What Good Citizenship Can Do for Reproducibility in Science

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   ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

3. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   ...Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven ChRs derived...

4. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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   ...published work meets the accepted standards of reporting in the discipline and reduces the dissemination...

5. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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   Blog Post

   ... the journal’s specific author guidelines for reporting your materials. If you deposit a plasmid with ...

6. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   ...plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify...

7. CASTing Off for New Shores in Human Genome Editing

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   ...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....

8. Plasmids 101: TOPO Cloning

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   Blog Post

   ...limit (lower transformation efficiencies have been reported with longer incubation); however, you may need...

9. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   ... a fluorescent protein fusion, transcriptional reporter, and loss-of-function allele in just one injection...

10. Management for Scientists: Delegating is Key

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    Blog Post

    ...shared document. Update frequently with progress reports, meeting minutes and plan changes. Written documentation...

11. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post

    ...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms...

12. Hot Plasmids: FRET-Based Biosensors

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    Blog Post

    ...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...

13. CRISPR 101: Multiplex Expression of gRNAs

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    ...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central...

14. Luminescent Imaging with Nano-lanterns

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    Blog Post

    ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...

15. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post

    ...retina upon intravitreal injection." Scientific reports 7 (2017): 45329. PubMed PMID: 28361998. PubMed ...

16. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    Blog Post

    ...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...

17. Summer SciComm Series: A PhD in Science Communication

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    Blog Post

    ...to all the optional sexual health sessions and reported the intel back to my shyer classmates. For my ...

18. DAP arrays for multiplex gene editing

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    Blog Post

    ...Multiplex expression of gRNAs Four Base Editing Reporters to Monitor and Enrich Editing in Real Time Prime...

19. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post

    ...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...

20. K. phaffii: Rising to the Occasion

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    Blog Post

    ...important gene co‑expression modules. Biomedical Reports 7.2 (2017): 153-158. DOI: 10.3892/br.2017.941 Bernauer...