We narrowed to 361 results for: reporter
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TypeBlog Post...lifelong hobby for Caitlin Runne-Janczy. “I wrote reports for fun in fourth grade because I was weird like...
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Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post... self-labeling tools: Tetracysteine: The first reported chemical labeling tag, from Roger Tsien’s lab ...collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...transgenic mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically...using 2-photon microscopy as this hasn't been well reported for many fluorescent proteins. Additional fluorescent... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al.... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post... the journal’s specific author guidelines for reporting your materials. If you deposit a plasmid with ... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven ChRs derived... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...published work meets the accepted standards of reporting in the discipline and reduces the dissemination... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog Post...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from... -
Plasmids 101: TOPO Cloning
TypeBlog Post...limit (lower transformation efficiencies have been reported with longer incubation); however, you may need... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post... a fluorescent protein fusion, transcriptional reporter, and loss-of-function allele in just one injection... -
Management for Scientists: Delegating is Key
TypeBlog Post...shared document. Update frequently with progress reports, meeting minutes and plan changes. Written documentation... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central... -
Caltech Systemic Capsids
TypeCollection...tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ... -
K. phaffii: Rising to the Occasion
TypeBlog Post...important gene co‑expression modules. Biomedical Reports 7.2 (2017): 153-158. DOI: 10.3892/br.2017.941 Bernauer... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...retina upon intravitreal injection." Scientific reports 7 (2017): 45329. PubMed PMID: 28361998. PubMed ... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...