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We narrowed to 254 results for: tre promoter

Showing: 221 - 240 of 254 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    ...from a replicating adenovirus using native viral promoters. Mol Ther, 7(4), 526–534. https:/doi.org/10.1016...as other intracellularly-exposed proteins and downstream cellular events, all recruit strong innate and...

  2. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow...that salaries may even double. The University of Notre Dame sets no minimum, stating that while they recommend...

  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional...introduced anywhere in the plasmid (albeit at extremely low frequency), and these could interfere with...

  4. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...When you go on interviews or are looking for a promotion, have some solid examples of times when you have...natural inclination for this skill that you could strengthen. The right set of transferable skills, coupled...other just for the sake of disagreeing. It can be extremely toxic in a lab or work environment if left unchecked...conflict resolution is likely not one of your strengths. Empathizing with people, especially people with...conflicts within a given organization, it can help tremendously to have as much context as possible. When I ...recognize that conflict resolution may never be a strength for you. But if you are a problem solver, and ...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We use a single polycistronic...conversion of a target ‘A’ to ‘I’ (inosine), which is treated as ‘G’ by cellular polymerases. Subsequently, an...developed using the hAPOBEC3 deaminase can edit in a stretched window ranging from 1-17 bp relative to the PAM...

  6. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but...pSer-incorporation on enzyme function through phosphatase treatment. If a candidate phosphatase is unknown, CIP or...

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene. Here, the target window is not...window upstream of the transcription start site (TSS), while for CRISPRi, a ~100nt window downstream of the...ability of the cell to use an alternative ATG downstream of the annotated start codon. Likewise, we avoid...

  8. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...sites on the target DNA, and thus inactivating the promoter. Image reused with permission from Neumann et ...this proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of...with a GFP nanobody (Figure 1). This enrichment streamlines the purification and minimizes sample loss while...

  9. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... 20-mer is not G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion...each sample and sequence each colony using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC...non-deletion band”) and another set of primers upstream and downstream of the sgRNA cleavage sites (“deletion ...sequences consist of a 20-mer (“protospacer sequence”) upstream of an “NGG” sequence (“protospacer adjacent motif...consists of DMEM supplemented with 2% penicillin/ streptomycin and 1% L-glutamine, which is used for MEL cells...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...integration at regions of open chromatin, especially at promoter or exonic regions. If the cell’s chromatin structure...piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no. 9, pp. 525–33, Sep. 2015...

  11. p53 Pathway

    Type
    Collection
    ... the best-characterized effects of p53 are in promoting cell cycle arrest, apoptosis, or senescence in...suppressor activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation. p53...factor and tumor suppressor activated by cellular stress. Cancer...

  12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...expression from rAAVs also depends on your choice of promoter/enhancer. Table 1: AAV capsids and receptors. ...developed from prior natural exposure or rAAV treatment, which can pose problems for gene therapy applications...therapeutic outer retinal gene delivery from the vitreous. Science Translational Medicine, 5(189), 189ra76...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ...recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis...of AAVs in humans AAVs are already being used to treat disease in humans and additional improvements are...transduction efficiency, and safety profiles should strengthen its appeal for the scientific and medical communities...

  14. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...example, piggyBac and Tol2 are best for screening promoters and enhancers since they are biased to insert ...from the donor molecule, and inserts it ~50 bp downstream of QCascade’s genomic target site (Fig. 3B). ...from the donor molecule, and inserts it ~50 bp downstream of QCascade’s genomic target site.  T-RL= transposon...

  15. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...activates hypoxia inducing factor (HIF-1), which promotes the production of VEGF to increase angiogenesis...labor with up and coming immunotherapy treatments. As treatments enter the clinic, physicians and researchers...more vasculature to feed themselves, use the blood stream to commute to and invade multiple organs, and most...cause, manifestation, diagnosis, recurrence, and treatment of cancer have been extensively explored. Biologists...compromised either due to age, infections, or other strenuous biological changes. In contrast to the acute immune... cytokines like TGFβ and IL-6. However, when strengthened through efforts to boost the activity of the... ability of even these conventional antitumor treatment strategies can be enhanced. Types of immunotherapeutics...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...spectrum. When photons are absorbed by matter, they promote electrons within atoms to a higher energy state...fluorescence. Another way to label proteins is through treating cells with fluorophore-conjugated antibodies that... fluorophore's brightness refers to the signal strength of the emitted photons detected by the cytometer...

  17. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...iPSC-derived osteoblasts and osteoclasts together promote bone regeneration in 3D biomaterials. Sci Rep, ...examples of transdifferentiation in vitro where treatment of mouse fibroblasts with 5-azacytidine led to...Mukherjee. Kusumika (Kushi) Mukherjee is the Editor of Trends in Pharmacological Sciences, a Cell Press reviews...adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss. Nature, 2010. 464(7292): p. 1149...expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia. Nat Med, 2000. 6(5):... PMID: 18754011. 43. Zhou, Q. and D.A. Melton, Extreme makeover: converting one cell into another. Cell...

  18. Typing CRISPR Systems

    Type
    Blog Post
    ...because it can process multiple gRNAs under a single promoter, allowing for easy multiplexing. The double-stranded...bacterial and archaeal immune systems has led to extreme sequence divergence that requires a multi-pronged...subtypes, A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first... CRISPR-Cas: beyond the Cas10 effector complex. Trends in Biochemical Sciences, 49(1), 28–37. https://...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...sequence (PFS). In bacteria, Cas13 targeting also promotes non-specific RNA cleavage, but this non-specific...mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others) Drosophila Plants (monocots...20 bp in length and paste them into contiguous stretches of DNA known as CRISPR arrays. Separate Cas proteins...proteins or RNAs through ChIP (enChIP) or biotin-streptavidin pulldown (CAPTURE.) Tag : Multiple methods make...exploring therapeutic applications of CRISPR to treat genetic disease, with the caveat that off-target...

  20. Promoters

    Type
    Guide
    ...Proximal Promoter Further upstream from the core promoter you will find the proximal promoter which contains...Reference Molecular Biology Reference Promoters Promoters Definition A promoter is a region of DNA where transcription...silencers. Promoter Regions There are three main portions that make up a promoter: core promoter, proximal...bind. Distal Promoter The final portion of the promoter region is called the distal promoter which is upstream...mammalian promoter PGK Constitutive Mammalian promoter from phospholycerate kinase gene TRE Inducible ...Specific Drosophila promoter containing Gal4 binding sites Bacterial Promoters Promoters in bacteria contain...tryptophan Promoter from E. coli tryptophan operon Ptac Regulated like the lac promoter Hybrid promoter of lac...
Showing: 221 - 240 of 254 results