We narrowed to 333 results for: uros
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TypeBlog Post... forget that this technique isn’t limited to neuroscience. In fact, precise light-based control of biological...
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Plasmids 101: Mammalian Vectors
TypeBlog Post...80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK, Jurkat T cells Inhibits protein... -
Deisseroth INTRSECT Collection
TypeCollection...2020. Prefrontal somatostatin interneurons encode fear memory. Nat Neurosci. 23(1):61-74. PubMed (Link opens...defined dopamine neuron subtypes using intersectional genetic approaches. Nat Neurosci. 21(9):1260-1271...cholecystokinin interneurons in the basal amygdala facilitates fear extinction. eNeuro. 6(6). PubMed (...to single-component cellular definitions (e.g. neurons defined by a single gene or projection). Intersectional...Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed excitation...excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786. PubMed (Link opens in...Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit... -
Lentivirus Plasmids
TypeCollection...Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin selection. See plasmid 24150...Diane Mathis 21915 Tet-pLKO-puro 3rd Inducible expression of shRNA with puromycin selection. See plasmid 21916...Eric Campeau 39481 pLenti-puro 3rd CMV-driven expression of cDNA with puromycin selection. Ie-Ming Shih ...pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin selection. Kevin ...17448 pLenti CMV GFP Puro (658-5) 3rd eGFP expression with CMV promoter and puromycin selection. See Campeau...19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion with puromycin resistance. Can be used for cDNA expression. David...for GFP reporter. Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive... -
AAV Q&A with Tim Miles
TypeBlog Post...microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and... -
Clear and Accessible Writing
TypeBlog Post...guidelines. Which is why, at the Society for Neuroscience conference in November 2023, we hosted our very... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post... a decidedly uncomfortable 45°C). Their more adventurous cousins are the extremophiles - the legends who... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post... AAV-mediated delivery to iPS-derived cortical neurons, dCasRx-mediated exon skipping improved the ratio... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...journals, has joined a pilot project from the Neuroscience Information Framework (NIF) called the Resource... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post...disease in question – cardiomyocytes, adipocytes or neurons, for instance – to see how those mutations influence... -
Kazuhiro Oka Lentiviral Vectors
TypeCollection...pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255 Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase...pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter...promoter pCDH-EF1-copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 ... promoter pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter...increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene of interest from the CB promoter...the mouse U6 promoter pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro 73022 Expresses Nluc and copGFP from the EF1 promoter...from the EF1 promoter pCDH-EF1s-Nluc-P2A-copGFP-T2A-Puro 73032 Expresses Nluc and copGFP from a truncated... -
Rett Syndrome
TypeCollection..., an immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific...Background Rett Syndrome Rett syndrome is a neurodevelopmental disorder that presents in early toddlerhood...contrast to the sustained loss of skills in neurodegenerative conditions, the regressive period in Rett ...determines a minimally functional MECP2 that rescues neurological defects of Rett syndrome. (Link opens in a new... PMID: 24399845 Guy et al. 2007. Reversal of Neurological Defects in a Mouse Model of Rett Syndrome. Science...girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens in a new window) PMID...progressive encephalopathy in boys and MECP2 mutations. Neurology . 67, 164–166. (Link opens in a new window) PMID... -
What's New in CRISPR - November 2021
TypeBlog Post...Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1. They found that this system, without... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...many FRET-opsin GEVIs. With an increase in the neuron’s membrane potential, Ace absorbance increases and... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...multiple cell types: fibroblasts, myofibroblasts, neuroendocrine, adipose, immune, inflammatory, angiogenic ... -
Tetracycline Inducible Expression
TypeCollection...21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. ...glutamatergic neurons via human NGN2 expression. See more from the iPSC Neurodegenerative Disease Initiative...TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and...and design of inducible shRNA expression, with puromycin selection. See Plasmid #85973 for blasticidin ... TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression...Tet-On 3G rtTA TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression...inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE David Root 100521... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...using CRISPR–dCas9-activator transgenic mice. Nat Neurosci 21:440–446 . https://doi.org/10.1038/s41593-017... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell clone selection. For more... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage sensors), to name a few. Find...