We narrowed to 336 results for: uros

...When Hans was finishing up his PhD in Molecular Neuroscience at the University of Iowa, he was like a lot...

...antibodies, so we're partnering with leading neuroscience and antibody experts to expand this collection...

...circulation. In Old World monkeys it actually targets neurons, and has 45-fold higher expression levels compared...

...including smell, taste, sight, inflammation, and neurotransmission. But with approximately 950 different human...

...different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein reporters...

...and carry the metallothionein promoter (e.g. pMT-puro). Worm vectors There are several approaches to studying...

...selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro - ...Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning...plasmids for Drosophila expression pMT-puro - Inducible gene expression under...mCherry selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1...lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA...lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral...

...targeting efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n...2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A...) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR ...PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; ...pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552 plasmid (#60958...-mediated expression of an sgRNA targeting the neuronal-specific gene NeuN. As a control they designed...

...audiences. She focuses at Science on developmental neurobiology and plant sciences, viewed through the lens ...

...is effective in sensitive cells such as living neurons, and can be fused to other fluorescent proteins...

...Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is ...

... (GEVIs) change fluorescence in real time when neurons or muscle cells transmit electrical information...

...immunogenicity prediction of therapeutic proteins. European journal of pharmaceutics and biopharmaceutics ...

...predicted cell type (e.g., axon-like projections for neuron predicted differentiation), and 15/17 morphologically...

...length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a Differences:  Distinct...

...disease mutation in basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. 2018; see ...

... if you need to. Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection...

...R-body producing recombinant E. coli on paramecia. European Journal of Protistology 48, 290–296 (2012). PubMed...

... forget that this technique isn’t limited to neuroscience. In fact, precise light-based control of biological...

...80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK, Jurkat T cells Inhibits protein...