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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5 results for: uros

Showing: 1 - 5 of 5 results

  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...kinase promoter drives expression of puromycin. Puro R Puromycin resistance gene for selection of pLKO...sulfate, and puromycin are located in the “Appendix”. F.2. Determining the Optimal Puromycin Concentration...differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin concentration...to fresh puromycin-containing media every other day. f. The minimum concentration of puromycin that results...with finer increments of puromycin to determine a more precise optimal puromycin concentration.) F.3. Protocol...expression of the puromycin resistance gene. f. To select for infected cells, add puromycin to the media at...positive control for the puromycin selection. Days 4+: g. Change to fresh puromycin-containing media as needed...

  2. Colony Formation Titering Assay

    Type
    Protocol
    ... . The following day, cells were treated with puromycin. Colonies were allowed to grow in the presence...

  3. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... Linear, Transfection Grade, VWR 75800-188 10% Pluronic F-18, Thermo Fisher 24040032 Glutagro, Corning...BCD TFX 1000 mL BalanCD HEK293 Media 10 mL 10% Pluronic-F68 40 mL 200 mM Glutagro Do not add selective...

  4. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a stable...preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation DMEM Complete...

  5. Kit Free RNA Extraction

    Type
    Protocol
    ...mM Sodium Citrate, pH 7.0 0.5% (wt/vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol®...
Showing: 1 - 5 of 5 results