We narrowed to 1,073 results for: TOR;

...and local movements predominate (e.g. a basal progenitor cell migrating to the epithelium). At this stage...fluorescent proteins can and have been used to monitor cellular movements in the immune system. The techniques...cell imaging, giving researchers the ability to monitor cellular movements in real time. This technique...host-pathogen interactions leading to the understanding of effector function in infected tissues. Before the use of...pathogens and fluorescent cells enables scientists to monitor cell interactions in diverse tissues, including...reveals limited antigen presentation and T cell effector function in mycobacterial granulomas." Immunity...PMCID: PMC4401519. 13. Auffray, Cedric, et al. "Monitoring of blood vessels and tissues by a population ...

...pH. We compared the dry ice-stored samples to controls that were only stored in a -80°C freezer and not...of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results showed that...mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and...reading a publication describing how short term storage on dry ice can shift sample pH, we wondered whether...medium, which is buffered and contains the pH indicator phenol red. We froze samples in a -80°C freezer...freezer and then moved them to a closed dry ice storage container for 48 hours (to replicate how our viruses...from the dry ice). After 48 hours of CO2/dry ice storage, we then thawed the samples at room temperature...

... taking up a position on the Addgene Board of Directors. We're excited to watch both Addgene and Sample...How to Be A Good Manager from Addgene Executive Director Joanne Kamens Resources on the Addgene Website... Check Out the Other Members of our Board of Directors Become a Part of the Addgene Community ...

... a Good Mentor? Choosing a Good Mentor for Scientists Will You Be My Mentor?   I Have a Mentor: What now...your own peer mentoring circle. In a Peer Mentoring Group all participants act as both mentors and mentees...Addgene Blog Mentoring for Scientists Series. I have been thinking a lot about Mentoring for over 10 years...mentees. So I experimented with a group mentoring format where 1 mentor met with a group of mentees to get ...her mentoring style. While working on this project, I read the book “Every Other Thursday: Stories and...success. Their stories made it clear to me that, executed correctly, the group mentoring format could work...Science (AWIS) mentoring programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees...

...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in...transferred in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...in-frame to these vectors. Plasmid... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger... zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290...

...transcription factors to increase gene expression. CRISPRa methods vary in their transcriptional activators: some...potent transcriptional activators (p65 and Rta). These transcriptional activators work in tandem to recruit...generally thought of as the “first generation” CRISPR activator. While it requires a relatively simple construct...modest gene activation. Synergistic Activation Mediator (SAM) Description SAM uses specially engineered...levels of gene activation compared to other CRISPR activators, making it a popular method for gene activation... SunTag performs better than first generation activators while showing lower activation levels than SAM...transcription. This complex consists of the VP64 activator used in other CRISPR activation methods, as well...

...adenine base editors with improved editing in cell lines Nicole Gaudelli, the Associate Director and Head ...Adenine base editors (ABE) mediate A•T-to-G•C base changes (Figure 1), but it can be challenging to make...to create the eighth generation of adenine base editors, called ABE8 (Gaudelli et al., 2020). The generated...to increase the targeting scope of adenine base editors The most commonly used S. pyogenes Cas9 requires...targeting scope for base editing.  Try ABE8 base editors for your research! Base editing in primary human...human cells  Cell therapy approaches can use base editors to correct mutations in stem cells and return the...Reducing off-target editing in ABE8s While base editors are precise molecular tools, they do have undesired...

...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering...systematically established way of making an all-in-one vector for multiplex genome engineering.” Now, they’...ease and efficiency for all-in-one CRISPR/Cas9 vectors expressing up to seven guide RNAs (gRNAs). Sakuma...Schematic overview of the all-in-one CRISPR/Cas9 vector construction system for multiplex genome engineering...expression cassettes of the gRNAs into a single vector in tandem. They went on to show that their CRISPR...CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully to target multiple sites in cultured...it has not yet been demonstrated, they say the vectors are also likely to work in animal embryos based...

...increasing CRISPR editing efficiency, new CRISPR base editors, DIY DNA ladders. You can find additional hot plasmids... Ouabain Selection  Hot Plasmid 3: CRISPR base editors with enhanced range and specificity. Listen to ...to to the Segmennt of Improved CRISPR Base Editors Hot Plasmid 4: pPSU Plasmids for generating DNA molecular...Out All of Our Hot Plasmids Articles Browse the Repository  ...

...unlikely receptor for PHP.B, and how the Deverman and Wilson labs determined LY6A was a receptor used by....B uses the LY6A receptor to gain access to the CNS. The bottom line of viral vector permissiveness While...pathogens to the CNS, but this also stops viral vectors, such as adeno-associated virus (AAV), from reaching...injection into the bloodstream, but requires a high vector dose and is unable to reach all regions of the ...identified lymphocyte antigen 6 complex (LY6A) as a receptor for PHP.B. Read on to learn how the design of ...also known as stem cell antigen-1 (SCA-1), is the receptor used by PHP.B to reach the CNS; yet LY6A is an...Wilson Labs independently identified LY6A as a receptor for PHP.B by looking for a unique sequence of ...