We narrowed to 658 results for: CHI;

... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...

...to your antibody. This chemical coupling can be achieved through several different mechanisms. Most take...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...

...Biology at Baylor College of Medicine and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...of Molecular and Cellular Biology, and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...

...While some of the statements may be slightly far reaching, remember that you are writing a proposal to test...secondary milestones to meet along the way to achieving each aim. A funded proposal typically lists the...

... or not proteins from the fission yeast, Schizosaccharomyces pombe, have the same function as their counterparts...yeast for young geneticists: A primer on the Schizosaccharomyces pombe model system. Genetics 201, 402-423...

...musculus (mouse), R. norvegicus (rat), G. gallus (chicken), B. taurus (bovine), X. laevis (frog), D. rerio...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...

...inducible promoters, light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas...Mayo-Muñoz D, He F, Jørgensen J, Madsen P, Bhoobalan-Chitty Y, Peng X (2018) Anti-CRISPR-Based and CRISPR-Based...

... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ...

...expression Generally speaking, one may want to achieve either inhibition or activation of neurons in a...Stoica et al., 2013). Vector delivery may also be achieved via injection into the liquor of the cerebral ...

...efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible genome editing with an engineered chimeric Cas9. Nat Commun 14, 6175 (2023). DOI:10.1038/...

... expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of...Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui Zeng Additional Resrouces...

...relatively high efficiency. These alterations are achieved through DNA degradation, thanks to the unique .... Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better option. To...

...10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel, ... Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R., Conlon, T.,...

...decide what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere...weight of your library plasmids. As long as you achieve sufficient coverage from your transformation, any...

...components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs...Expression Systems Recombinant protein expression in Escherichia coli: advances and challenges Production of recombinant...

...still virgins. Females begin mating ~6 hours after hatching into their adult phase. It is important to clear...before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after setting up...

...is to make it detectable. Visualization can be achieved using fluorescent microscopy - even in living ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow...

...research that aligns with your interests and goals.  Reaching Out to Potential PIs Even if you’re applying to...you determine if the program is the right fit. Reaching out to PIs also means doing plenty of mini-interviews...

...and departments will have qPCR machines available for use. ddPCR machines are not as commonly available...

...setting—especially if job applications require teaching experience or high numbers of publications, which...developmental biology and is applying this towards researching neurodegenerative diseases.   Additional resources...

...Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production and purification...

...behavior of cancer cells, attacking the cellular machinery necessary for division. This approach also kills... University of California, San Francisco, is approaching the Ras problem by exploiting the different mutant...

... of "The world’s biggest and best children’s museum", The Children's Museum of Indianapolis. I drove passed...

...Determine what type of outcome you are trying to achieve: Do you want to permanently knock out or knock-... All of these outcomes can be most effectively achieved with different CRISPR components. Select the appropriate...

...last month it’s been viewed by users in the US, China, India, Canada, the UK, South Korea, and beyond!...protocols So that’s great, but what if someone in China improved upon that protocol? How would the thousands...

...with Golden Gate MoClo. The barrier for those switching to Golden Gate is a conceptual barrier. Sometimes... of the university impacts the number of women reaching senior positions. There has been a lot of engagement...

...that you use the same solution for blanking your machine!  Finally, many NanoDrop systems now provide full-spectrum...are no substitute for sound techniques, routine machine maintenance, and critical interpretation of the...

...using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab. gRNAs can be in vitro transcribed from ssDNA...publicly available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins...

... Chemistry at Connecticut College who has been teaching and communicating science for over two decades...as pertinent now as it was over 300 years ago. Teaching by example No examination of the state of science...