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We narrowed to 650 results for: CHI;

Showing: 361 - 390 of 650 results

  1. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...inducible promoters, light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas...Mayo-Muñoz D, He F, Jørgensen J, Madsen P, Bhoobalan-Chitty Y, Peng X (2018) Anti-CRISPR-Based and CRISPR-Based...

  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...musculus (mouse), R. norvegicus (rat), G. gallus (chicken), B. taurus (bovine), X. laevis (frog), D. rerio...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...

  5. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible genome editing with an engineered chimeric Cas9. Nat Commun 14, 6175 (2023). DOI:10.1038/...

  6. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ...

  7. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of...Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui Zeng Additional Resrouces...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs...Expression Systems Recombinant protein expression in Escherichia coli: advances and challenges Production of recombinant...

  12. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow...

  13. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...relatively high efficiency. These alterations are achieved through DNA degradation, thanks to the unique .... Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better option. To...

  14. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel, ... Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R., Conlon, T.,...

  15. Pooled Library Amplifications

    Type
    Blog Post
    ...decide what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere...weight of your library plasmids. As long as you achieve sufficient coverage from your transformation, any...

  16. PhD Applications After COVID

    Type
    Blog Post
    ...research that aligns with your interests and goals.  Reaching Out to Potential PIs Even if you’re applying to...you determine if the program is the right fit. Reaching out to PIs also means doing plenty of mini-interviews...

  17. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...setting—especially if job applications require teaching experience or high numbers of publications, which...developmental biology and is applying this towards researching neurodegenerative diseases.   Additional resources...

  24. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....experience.  Can combines cons of multiple medias. Switching from one media type to another is cognitively ...

  25. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab. gRNAs can be in vitro transcribed from ssDNA...publicly available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins...

  26. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...limited in the level of resolution that they can achieve. This means that regardless of how powerful of ...evenly in all directions, aka isotropically. But achieving isotropic expansion depends on how well the gel...

  27. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...opportunities for his students. But he also found that his teaching responsibilities left him with limited time for...the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent training...

  28. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut.      Fig. 2: Heatmap indicating...when interpreting findings, particularly when researching ancestry groups. And, of course, it’s imperative...

  30. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 1983;100:293...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...
Showing: 361 - 390 of 650 results